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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

In-Depth Venome of the Brazilian Rattlesnake Crotalus durissus terrificus: An Integrative Approach Combining Its Venom Gland Transcriptome and Venom Proteome

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Wiezel, Gisele A. [1] ; Shibao, Priscila Y. T. [1] ; Cologna, Camila T. [1] ; Morandi Filho, Romualdo [2] ; Ueira-Vieira, Carlos [2] ; De Pauw, Edwin [3] ; Quinton, Loic [3] ; Arantes, Eliane C. [1]
Total Authors: 8
[1] Univ Sao Paulo, Dept Phys & Chem, Sch Pharmaceut Sci Ribeirao Preto, Ave Cafe S-N, BR-14040903 Ribeirao Preto - Brazil
[2] Univ Fed Uberlandia, Genet Lab, Inst Biotechnol, Rua Acre S-N, BR-38400902 Uberlandia, MG - Brazil
[3] Univ Liege, Lab Mass Spectrometry, MolSys Res Unit, Dept Chem, Bat B6c, B-4000 Liege - Belgium
Total Affiliations: 3
Document type: Journal article
Source: JOURNAL OF PROTEOME RESEARCH; v. 17, n. 11, p. 3941-3958, NOV 2018.
Web of Science Citations: 2

Snake venoms are complex mixtures mainly composed of proteins and small peptides. Crotoxin is one of the most studied components from Crotalus venoms, but many other components are less known due to their low abundance. The venome of Crotalus durissus terrificus, the most lethal Brazilian snake, was investigated by combining its venom gland transcriptome and proteome to create a holistic database of venom compounds unraveling novel toxins. We constructed a cDNA library from C. d. terrificus venom gland using the Illumina platform and investigated its venom proteome through high resolution liquid chromotography-tandem mass spectrometry. After integrating data from both data sets, more than 30 venom components classes were identified by the transcriptomic analysis and 15 of them were detected in the venom proteome. However, few of them (PLA2, SVMP, SVSP, and VEGF) were relatively abundant. Furthermore, only seven expressed transcripts contributed to similar to 82% and similar to 73% of the abundance in the transcriptome and proteome, respectively. Additionally, novel venom proteins are reported, and we highlight the importance of using different databases to perform the data integration and discuss the structure of the venom components-related transcripts identified. Concluding, this research paves the way for novel investigations and discovery of future pharmacological agents or targets in the antivenom therapy. (AU)

FAPESP's process: 11/23236-4 - Native and recombinant animal toxins: functional, structural and molecular analysis
Grantee:Suely Vilela
Support type: Research Projects - Thematic Grants
FAPESP's process: 17/00586-6 - Characterization of a phospholipase A2 inhibitor from the Crotalus durissus terrificus venom gland: a possible adjuvant in the antivenom therapy
Grantee:Gisele Adriano Wiezel
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 15/18432-0 - Bioprospection of animal toxins with biotechnological interest through omic tools
Grantee:Eliane Candiani Arantes Braga
Support type: Regular Research Grants
FAPESP's process: 14/15644-3 - Rhinella schneideri mucous gland trascriptome
Grantee:Priscila Yumi Tanaka Shibao
Support type: Scholarships in Brazil - Master
FAPESP's process: 13/26200-6 - Venomics Plataform of Pachycondyla villosa Proteome, Transcriptome, Cloning and Expression of toxins with biotechnological potential
Grantee:Camila Takeno Cologna
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 15/17466-8 - Venomics Platform: Pachycondyla villosa venom proteome and integrated analysis of omics data
Grantee:Camila Takeno Cologna
Support type: Scholarships abroad - Research Internship - Post-doctor