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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Prevention of Corneal Neovascularization by Adenovirus Encoding Human Vascular Endothelial Growth Factor Soluble Receptor (s-VEGFR1) in Lacrimal Gland

Texto completo
Autor(es):
Nominato, Luis Fernando [1] ; Dias, Ana Carolina [1] ; Dias, Lara Cristina [1] ; Fantucci, Marina Zilio [1] ; Costa Mendes da Silva, Lilian Eslaine [1] ; Murashima, Adriana de Andrade [1] ; Rocha, Eduardo Melani [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Ophthalmol Otorhinolaryngol & Head & Neck Su, Bandeirantes Ave 3900, BR-14049900 Ribeirao Preto, SP - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE; v. 59, n. 15, p. 6036-6044, DEC 2018.
Citações Web of Science: 1
Resumo

PURPOSE. The aims of this study were (1) to determine the efficacy of adenovirus vector serotype 5 (Ad) encoding human soluble VEGF receptor 1 (s-VEGFR1) gene transfer to the lacrimal gland (LG); (2) to investigate whether expression of s-VEGFR1 prevents corneal neovascularization (CNV) induced by alkali burns; and (3) to evaluate the safety of the procedure. METHODS. AdVEGFR1 vectors (25 mu L, 1 3 10 10 pfu/mL) were injected in the right LGs of rats and were compared with AdNull vector (25 mu L, 1 3 10 10 pfu/mL) or 25 mu L of saline (Control) before cornea alkali burns with 1 M NaOH. After 7 days, CNV was documented at the slit lamp. Tear secretion was measured with phenol red threads. The animals were tested for sVEGFR1 mRNA and protein in the LG by quantitative (q) PCR and immunohistochemistry staining, respectively. qPCR was used to compare the mRNA levels of IL-1b, IL-6, and TNF-alpha in the LG and ipsilateral trigeminal ganglion (TG). RESULTS. Ad-VEGFR1 transfected 83% (10/12) of the rats. VEGFR1 was present in LG acinar cells. CNV was prevented in 9 of 12 animals in the Ad-VEGFR1 group, compared with the AdNull (3: 10) and Control groups (1: 10) (P = 0.0317). The tear secretion and cytokine mRNA levels in the LG and TG were similar in all three groups (P > 0.05). CONCLUSIONS. Adenoviral vector gene transfer was safe for LG structure and function. The LG as the target tissue showed local expression of human s-VEGFR1, and CNV was prevented in most of the eyes exposed to alkali burns. (AU)

Processo FAPESP: 14/22451-7 - Sistemas de liberação sustentada e direcionada de fármacos para o tecido epitelial
Beneficiário:Renata Fonseca Vianna Lopez
Linha de fomento: Auxílio à Pesquisa - Temático