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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Prevention of Corneal Neovascularization by Adenovirus Encoding Human Vascular Endothelial Growth Factor Soluble Receptor (s-VEGFR1) in Lacrimal Gland

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Author(s):
Nominato, Luis Fernando [1] ; Dias, Ana Carolina [1] ; Dias, Lara Cristina [1] ; Fantucci, Marina Zilio [1] ; Costa Mendes da Silva, Lilian Eslaine [1] ; Murashima, Adriana de Andrade [1] ; Rocha, Eduardo Melani [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Ophthalmol Otorhinolaryngol & Head & Neck Su, Bandeirantes Ave 3900, BR-14049900 Ribeirao Preto, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE; v. 59, n. 15, p. 6036-6044, DEC 2018.
Web of Science Citations: 1
Abstract

PURPOSE. The aims of this study were (1) to determine the efficacy of adenovirus vector serotype 5 (Ad) encoding human soluble VEGF receptor 1 (s-VEGFR1) gene transfer to the lacrimal gland (LG); (2) to investigate whether expression of s-VEGFR1 prevents corneal neovascularization (CNV) induced by alkali burns; and (3) to evaluate the safety of the procedure. METHODS. AdVEGFR1 vectors (25 mu L, 1 3 10 10 pfu/mL) were injected in the right LGs of rats and were compared with AdNull vector (25 mu L, 1 3 10 10 pfu/mL) or 25 mu L of saline (Control) before cornea alkali burns with 1 M NaOH. After 7 days, CNV was documented at the slit lamp. Tear secretion was measured with phenol red threads. The animals were tested for sVEGFR1 mRNA and protein in the LG by quantitative (q) PCR and immunohistochemistry staining, respectively. qPCR was used to compare the mRNA levels of IL-1b, IL-6, and TNF-alpha in the LG and ipsilateral trigeminal ganglion (TG). RESULTS. Ad-VEGFR1 transfected 83% (10/12) of the rats. VEGFR1 was present in LG acinar cells. CNV was prevented in 9 of 12 animals in the Ad-VEGFR1 group, compared with the AdNull (3: 10) and Control groups (1: 10) (P = 0.0317). The tear secretion and cytokine mRNA levels in the LG and TG were similar in all three groups (P > 0.05). CONCLUSIONS. Adenoviral vector gene transfer was safe for LG structure and function. The LG as the target tissue showed local expression of human s-VEGFR1, and CNV was prevented in most of the eyes exposed to alkali burns. (AU)

FAPESP's process: 14/22451-7 - Sustained drug delivery systems targeting the epithelial tissue
Grantee:Renata Fonseca Vianna Lopez
Support type: Research Projects - Thematic Grants