Influence of polymorphisms in genes related to angiogenesis in the susceptibility to Hodgkin's Lymphoma

• Grant number: 14/24894-3
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): May 01, 2015
• Effective date (End): December 31, 2015
• Field of knowledge: Biological Sciences - Genetics - Human and Medical Genetics
• Principal Investigator: Gustavo Jacob Lourenço
• Grantee: Bruna de Albuquerque Murbach
• Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

The dependency and prognosis of Hodgkin lymphoma (HL) on angiogenesis (AG), characterized by overexpression of the vascular endothelial growth factor (VEGF) and vascular endothelial growth factor receptor 2 (VEGFR2) was described recently. However, distinct inherited abilities for AG, associated with genetic polymorphisms, may influence the VEGF production and the potential of VEGF ligation to VEGFR2. Thus, the objective of this study is the evaluate the roles of the polymorphisms c.-2055A>G (rs699947) and c.-614A>G (rs1570360) of the VEGF gene and c.-906T>C (rs2071559) and c.889G>A (rs2305948) of the VEGFR2 gene in the classical HL (cHL) occurrence and in clinical characteristics and survival. It will be evaluated 120 patients with cHL and 120 controls, matched to patients by age, gender and skin color, treated at the Hematology and Hemotherapy Center of the University of Campinas. The clinical information will be obtained in the patients' records by the researcher. The genotypes of the referred polymorphisms will be identified by real-time polymerase chain reaction, using TaqMan® assays. The statistical significance of the difference between groups will be calculated by Fischer's exact test or Ç2, and multiple logistic regressions. The cHL risk determination will be calculated by odds ratio considering a 95% confidence interval. The disease-free survival and overall survival times will be calculated using Kaplan-Meier estimate probabilities and differences between survival curves will be analyzed by the log-rank test. The prognostic impact of genotypes will be examined using univariate and multivariate Cox regression analyses. We believe that the results of this study could contribute to the knowledge of the etiology and physiopathology of the cHL, and these results also could identify individuals with a higher risk of disease and receive special attention to prevention, early disease diagnosis, and treatment.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MURBACH, BRUNA DE A.; DELAMAIN, MARCIA T.; DANIEL, VANESSA; DE SOUZA, CARMINO A.; LIMA, CARMEN S. P.; LOURENCO, GUSTAVO J.. Association between polymorphisms in angiogenesis-related genes and the prognosis of classical Hodgkin lymphoma. British Journal of Haematology, v. 185, n. 2, p. 366-370, APR 2019. (14/24894-3)