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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Crotalus durissus ruruima Snake Venom and a Phospholipase A(2) Isolated from This Venom Elicit Macrophages to Form Lipid Droplets and Synthesize Inflammatory Lipid Mediators

Texto completo
Autor(es):
Zulim de Carvalho, Ana Eduarda [1] ; Giannotti, Karina [1] ; Leiguez Junior, Elbio [1] ; Matsubara, Marcio [1] ; Dos Santos, Maria Cristina [2] ; Fortes-Dias, Consuelo Latorre [3] ; Teixeira, Catarina [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Butantan Inst, Sao Paulo, SP - Brazil
[2] Univ Amazonas, Dept Ciencias Fisiol, Manaus, Amazonas - Brazil
[3] Fundacao Ezequiel Dias, Belo Horizonte, MG - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF IMMUNOLOGY RESEARCH; v. 2019, NOV 4 2019.
Citações Web of Science: 0
Resumo

Viper snake Crotalus durissus ruruima (Cdr) is a subspecies found in northern area of Brazil. Among the snakes of Crotalus genus subspecies, the venom of Cdr presents highest level of crotoxin, which is the major component of Crotalus snake venoms, formed by two subunits (crotapotin and a phospholipase A(2) named CBr) and presents potent neurotoxic activity. Curiously, the venom of C. d. ruruima (CdrV) is better neutralized by antibothropic than by anticrotalic serum, strongly suggesting that this venom has similarities with venom of Bothrops genus snakes with regard to the ability to induce inflammation. Macrophages are cells with a central role in inflammatory and immunological responses. Upon inflammatory stimuli, these cells exhibit increased numbers of lipid droplets, which are key organelles in the synthesis and release of inflammatory mediators. However, the effects of CdrV and CBr in macrophage functions are unknown. We herein investigated the ability of CdrV and CBr to activate macrophages with focus on the formation of lipid droplets (LDs), synthesis of lipid mediators, and mechanisms involved in these effects. The involvement of LDs in PGE(2) biosynthesis was also assessed. Stimulation of murine macrophages with CdrV and CBr induced an increased number of LDs and release of prostanoids (PGE(2), PGD(2), and TXB2). Neither CdrV nor CBr induced the expression of COX-1 and COX-2 by macrophages. LDs induced by both CdrV and CBr are associated to PLIN2 recruitment and expression and were shown to be dependent on COX-1, but not COX-2 activity. Moreover, PGE(2) colocalized to CdrV- and CBr-induced LDs, revealing the role of these organelles as sites for the synthesis of prostanoids. These results evidence, for the first time, the ability of a whole snake venom to induce formation of LDs and the potential role of these organelles for the production of inflammatory mediators during envenomation by Crotalus snakes. (AU)

Processo FAPESP: 15/24701-3 - Estudo dos efeitos de uma fosfolipase a2 de veneno ofidico em células do tecido adiposo: adipogênese e resposta inflamatória
Beneficiário:Elbio Leiguez Junior
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 14/18549-1 - Caracterização dos efeitos de uma Fosfolipase A2 (MT-III) de veneno ofídico em células de músculo liso vascular em cultura: formação de células espumosas e mecanismos envolvidos
Beneficiário:Catarina de Fatima Pereira Teixeira
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 18/05637-0 - Identificação de peptídeos derivados da crotoxina com potencial antitumoral e imunomodulatório em modelo de melanoma cutâneo e de encefalomielite autoimune experimental.
Beneficiário:Karina Cristina Giannotti
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 15/50040-4 - Rational approach for searching molecular targets involved in inflammatory events and cell survival
Beneficiário:Ana Marisa Chudzinski-Tavassi
Linha de fomento: Auxílio à Pesquisa - Programa Centros de Pesquisa em Engenharia
Processo FAPESP: 00/11624-5 - Embriologia de Gastropoda: análise em microscopia de varredura laser confocal
Beneficiário:Toshie Kawano
Linha de fomento: Auxílio à Pesquisa - Programa Equipamentos Multiusuários