Human Cerebral Organoids and Fetal Brain Tissue Share Proteomic Similarities

Autor(es)

Nascimento, Juliana Minardi ^{[1, 2]} ; Saia-Cereda, Veronica M. ^[2] ; Sartore, Rafaela C. ^{[1, 3]} ; da Costa, Rodrigo Madeiro ^[1] ; Schitine, Clarissa S. ^{[4, 5]} ; Freitas, Hercules Rezende ^{[5, 6]} ; Murgu, Michael ^[7] ; de Melo Reis, Ricardo A. ^[5] ; Rehen, Stevens K. ^{[1, 4]} ; Martins-de-Souza, Daniel ^{[2, 8, 9]}

Afiliação do(s) autor(es)

^[1] DOr Inst Res & Educ IDOR, Rio De Janeiro - Brazil

^[2] Univ Estadual Campinas, Inst Biol, Dept Biochem & Tissue Biol, Lab Neuroprote, UNICAMP, Campinas, SP - Brazil

^[3] Natl Inst Traumatol & Orthoped, Rio De Janeiro - Brazil

^[4] Fed Univ Rio de Janeiro UFRJ, Inst Biomed Sci, Rio De Janeiro - Brazil

^[5] Fed Univ Rio de Janeiro UFRJ, Inst Biophys, Rio De Janeiro - Brazil

^[6] IBMR Univ Ctr, Sch Hlth Sci, Rio De Janeiro - Brazil

^[7] Waters Corp, Barueri - Brazil

^[8] Conselho Nacl Desenvolvimento Cient & Tecnol, Inst Nacl Biomarcadores Neuropsiquiatria INBION, Sao Paulo, SP - Brazil

^[9] Univ Estadual Campinas, Expt Med Res Cluster, Campinas - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY; v. 7, NOV 28 2019.
• Citações Web of Science: 0

Resumo

The limited access to functional human brain tissue has led to the development of stem cell-based alternative models. The differentiation of human pluripotent stem cells into cerebral organoids with self-organized architecture has created novel opportunities to study the early stages of the human cerebral formation. Here we applied state-of-the-art label-free shotgun proteomics to compare the proteome of stem cell-derived cerebral organoids to the human fetal brain. We identified 3,073 proteins associated with different developmental stages, from neural progenitors to neurons, astrocytes, or oligodendrocytes. The major protein groups are associated with neurogenesis, axon guidance, synaptogenesis, and cortical brain development. Glial cell proteins related to cell growth and maintenance, energy metabolism, cell communication, and signaling were also described. Our data support the variety of cells and neural network functional pathways observed within cell-derived cerebral organoids, confirming their usefulness as an alternative model. The characterization of brain organoid proteome is key to explore, in a dish, atypical and disrupted processes during brain development or neurodevelopmental, neurodegenerative, and neuropsychiatric diseases. (AU)

• Processo FAPESP: 14/21035-0 - Proteômica quantitativa de linhagens neurais e organóides cerebrais derivados de células tronco de pluripotência induzida de pacientes com esquizofrenia
• Beneficiário: Juliana Minardi Nascimento
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/10068-4 - EMU concedido no processo 13/08711-3: espectrômetro de massas Waters SYNAPT G2-Si HDMs + nanoACQUITY UPLC
• Beneficiário: Daniel Martins-de-Souza
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 13/08711-3 - Desenvolvimento de um teste preditivo para medicação bem sucedida e compreensão das bases moleculares da esquizofrenia através da proteômica
• Beneficiário: Daniel Martins-de-Souza
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 16/07332-7 - O papel das vias de sinalização 14-3-3 e Efrina na comunicação celular entre neurônios-astrócitos e funcionamento da sinapse tripartite
• Beneficiário: Verônica Aparecida Monteiro Saia Cereda
• Modalidade de apoio: Bolsas no Brasil - Doutorado