| Full text | |
| Author(s): |
Nascimento, Juliana Minardi
[1, 2]
;
Saia-Cereda, Veronica M.
[2]
;
Sartore, Rafaela C.
[1, 3]
;
da Costa, Rodrigo Madeiro
[1]
;
Schitine, Clarissa S.
[4, 5]
;
Freitas, Hercules Rezende
[5, 6]
;
Murgu, Michael
[7]
;
de Melo Reis, Ricardo A.
[5]
;
Rehen, Stevens K.
[1, 4]
;
Martins-de-Souza, Daniel
[2, 8, 9]
Total Authors: 10
|
| Affiliation: | [1] DOr Inst Res & Educ IDOR, Rio De Janeiro - Brazil
[2] Univ Estadual Campinas, Inst Biol, Dept Biochem & Tissue Biol, Lab Neuroprote, UNICAMP, Campinas, SP - Brazil
[3] Natl Inst Traumatol & Orthoped, Rio De Janeiro - Brazil
[4] Fed Univ Rio de Janeiro UFRJ, Inst Biomed Sci, Rio De Janeiro - Brazil
[5] Fed Univ Rio de Janeiro UFRJ, Inst Biophys, Rio De Janeiro - Brazil
[6] IBMR Univ Ctr, Sch Hlth Sci, Rio De Janeiro - Brazil
[7] Waters Corp, Barueri - Brazil
[8] Conselho Nacl Desenvolvimento Cient & Tecnol, Inst Nacl Biomarcadores Neuropsiquiatria INBION, Sao Paulo, SP - Brazil
[9] Univ Estadual Campinas, Expt Med Res Cluster, Campinas - Brazil
Total Affiliations: 9
|
| Document type: | Journal article |
| Source: | FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY; v. 7, NOV 28 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
The limited access to functional human brain tissue has led to the development of stem cell-based alternative models. The differentiation of human pluripotent stem cells into cerebral organoids with self-organized architecture has created novel opportunities to study the early stages of the human cerebral formation. Here we applied state-of-the-art label-free shotgun proteomics to compare the proteome of stem cell-derived cerebral organoids to the human fetal brain. We identified 3,073 proteins associated with different developmental stages, from neural progenitors to neurons, astrocytes, or oligodendrocytes. The major protein groups are associated with neurogenesis, axon guidance, synaptogenesis, and cortical brain development. Glial cell proteins related to cell growth and maintenance, energy metabolism, cell communication, and signaling were also described. Our data support the variety of cells and neural network functional pathways observed within cell-derived cerebral organoids, confirming their usefulness as an alternative model. The characterization of brain organoid proteome is key to explore, in a dish, atypical and disrupted processes during brain development or neurodevelopmental, neurodegenerative, and neuropsychiatric diseases. (AU) | |
| FAPESP's process: | 14/21035-0 - Quantitative proteomics in neural cell lines and organoids derived from induced pluripotent stem cells from schizophrenia patients |
| Grantee: | Juliana Minardi Nascimento |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 14/10068-4 - Multi-User Equipment approved in grant 13/08711-3: mass spectrometer waters SYNAPT G2-Si HDMS + nanoACQUITY UPLC |
| Grantee: | Daniel Martins-de-Souza |
| Support Opportunities: | Multi-user Equipment Program |
| FAPESP's process: | 13/08711-3 - Developing a predictive test for a successful medication response and understanding the molecular bases of schizophrenia through proteomics |
| Grantee: | Daniel Martins-de-Souza |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 16/07332-7 - The role of 14-3-3 and Ephrin signaling pathways on cellular communication between neurons-astrocytes and the functioning of the synapse tripartite |
| Grantee: | Verônica Aparecida Monteiro Saia Cereda |
| Support Opportunities: | Scholarships in Brazil - Doctorate |