Polyphenol-rich green tea extract induces thermogenesis in mice by a mechanism dependent on adiponectin signaling

Autor(es)

Bolin, Anaysa Paola ^[1] ; Sousa-Filho, Celso Pereira Batista ^[2] ; Marinovic, Marcelo Paradiso ^[1] ; Rodrigues, Alice Cristina ^[1] ; Otton, Rosemari ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, Sao Paulo - Brazil

^[2] Cruzeiro do Sul Univ, Interdisciplinary Postgrad Programme Hlth Sci, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF NUTRITIONAL BIOCHEMISTRY; v. 78, APR 2020.
• Citações Web of Science: 0

Resumo

Adiponectin is downregulated in obesity negatively impacting the thermogenesis and impairing white fat browning. Despite the notable effects of green tea (GT) extract in the enhancement of thermogenesis, if its effects are being mediated by adiponectin has been scarcely explored. For this purpose, we investigated the role of adiponectin in the thermogenic actions of GT extract by using an adiponectin-knockout mice model. Male wild-type (WT) and knockout (AdipoKO) C57Bl/6 mice (3 months) were divided into 6 groups: mice fed a standard diet+gavage with water (SD WT, and SD AdipoKO), high-fat diet (HFD)+gavage with water (HFD WT, and HFD AdipoKO), and HFD + gavage with 500 mg/kg of body weight (BW) of GT extract (HFD + GT WT, and HFD + GT AdipoKO). After 20 weeks of experimentation, mice were euthanized and adipose tissue was properly removed. Our findings indicate that treatment with GT extract reversed complications of obesity in WT mice by decreasing final BW gain, adiposity index, adipocyte size and insulin resistance (IR). However, the action of the GT extract was not effective in reversing those markers in the AdipoKO mice, although GT acts independently in the reversal of IR. GT-treatment induced enhancement in energy expenditure (EE), BAT thermogenesis, and promoted browning phenotype in the subcutaneous WAT (scWAT) of WT mice. On the other hand, the thermogenic program was markedly impaired in BAT and scWAT of AdipoKO mice. Our outcomes unveiled adiponectin as a key direct signal for GT extract inducing adaptive thermogenesis and browning in scWAT. (C) 2019 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 17/10531-4 - Investigação do potencial do chá verde em induzir o browning in vitro em pré-adipócitos e no tecido adiposo subcutâneo de camundongos obesos nocaute para adiponectina
• Beneficiário: Celso Pereira Batista Sousa Filho
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 11/19216-8 - Avaliação sistemática de parâmetros oxidativos e inflamatórios em ratos obesos suplementados cronicamente com extrato de chá verde (Camellia sinensis)
• Beneficiário: Rosemari Otton
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/12059-8 - Investigação do papel da adiponectina e da via beta-adrenérgica como mediadores dos efeitos antiobesidade dos polifenóis do chá verde
• Beneficiário: Rosemari Otton
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/20415-8 - Modulação do estresse de retículo endoplasmático de adipócitos e leucócitos de camundongos induzidos a obesidade e tratados cronicamente com extrato de chá verde (Camellia sinensis)
• Beneficiário: Anaysa Paola Bolin
• Modalidade de apoio: Bolsas no Brasil - Doutorado