Polyphenol-rich green tea extract induces thermogenesis in mice by a mechanism dependent on adiponectin signaling

Author(s)

Bolin, Anaysa Paola ^[1] ; Sousa-Filho, Celso Pereira Batista ^[2] ; Marinovic, Marcelo Paradiso ^[1] ; Rodrigues, Alice Cristina ^[1] ; Otton, Rosemari ^{[1, 2]}

Affiliation

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, Sao Paulo - Brazil

^[2] Cruzeiro do Sul Univ, Interdisciplinary Postgrad Programme Hlth Sci, Sao Paulo - Brazil

• Document type: Journal article
• Source: JOURNAL OF NUTRITIONAL BIOCHEMISTRY; v. 78, APR 2020.
• Web of Science Citations: 0

Abstract

Adiponectin is downregulated in obesity negatively impacting the thermogenesis and impairing white fat browning. Despite the notable effects of green tea (GT) extract in the enhancement of thermogenesis, if its effects are being mediated by adiponectin has been scarcely explored. For this purpose, we investigated the role of adiponectin in the thermogenic actions of GT extract by using an adiponectin-knockout mice model. Male wild-type (WT) and knockout (AdipoKO) C57Bl/6 mice (3 months) were divided into 6 groups: mice fed a standard diet+gavage with water (SD WT, and SD AdipoKO), high-fat diet (HFD)+gavage with water (HFD WT, and HFD AdipoKO), and HFD + gavage with 500 mg/kg of body weight (BW) of GT extract (HFD + GT WT, and HFD + GT AdipoKO). After 20 weeks of experimentation, mice were euthanized and adipose tissue was properly removed. Our findings indicate that treatment with GT extract reversed complications of obesity in WT mice by decreasing final BW gain, adiposity index, adipocyte size and insulin resistance (IR). However, the action of the GT extract was not effective in reversing those markers in the AdipoKO mice, although GT acts independently in the reversal of IR. GT-treatment induced enhancement in energy expenditure (EE), BAT thermogenesis, and promoted browning phenotype in the subcutaneous WAT (scWAT) of WT mice. On the other hand, the thermogenic program was markedly impaired in BAT and scWAT of AdipoKO mice. Our outcomes unveiled adiponectin as a key direct signal for GT extract inducing adaptive thermogenesis and browning in scWAT. (C) 2019 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 17/10531-4 - Investigation of the potential of green tea in inducing browning in vitro in pre-adipocytes and in the subcutaneous adipose tissue from obese adiponectin knockout mice
• Grantee: Celso Pereira Batista Sousa Filho
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 11/19216-8 - Systematic evaluation of oxidative and inflammatory parameters in obese rats chronically supplemented with green tea extract (Camellia sinensis)
• Grantee: Rosemari Otton
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/12059-8 - Investigation of adiponectin and beta-adrenergic pathway mediating the anti-obesity effects of green tea polyphenols
• Grantee: Rosemari Otton
• Support Opportunities: Regular Research Grants
• FAPESP's process: 12/20415-8 - Modulation of endoplasmic reticulum stress in adipocytes and leukocytes of mice induced to obesity and chronically treated with green tea extract (Camellia sinensis)
• Grantee: Anaysa Paola Bolin
• Support Opportunities: Scholarships in Brazil - Doctorate