Evidence for sub-functionalization of tandemly duplicated XPB nucleotide excision repair genes in Arabidopsis thaliana

Autor(es)

Masuda, Hana Paula ^{[1, 2]} ; Nakabashi, Myna ^[1] ; Morgante, Patricia G. ^{[1, 3]} ; Kajihara, Daniela ^{[1, 4]} ; de Setta, Nathalia ^{[1, 2]} ; Martins Menck, Carlos Frederico ^[5] ; Van Sluys, Marie-Anne ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biociencias, Dept Bot, Sao Paulo, SP - Brazil

^[2] Univ Fed ABC UFABC, Ctr Ciencias Nat & Humanas, Sao Bernardo Do Campo, SP - Brazil

^[3] Univ Estadual Paulista UNESP, Campus Registro, Sao Paulo, SP - Brazil

^[4] Univ Sao Paulo, Inst Coracao InCor, Lab Biol Vasc, Fac Med, Sao Paulo, SP - Brazil

^[5] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Gene; v. 754, SEP 5 2020.
• Citações Web of Science: 0

Resumo

Plants are continuously exposed to agents that can generate DNA lesions. Nucleotide Excision Repair (NER) is one of the repair pathways employed by plants to protect their genome, including from sunlight. The Xeroderma Pigmentosum type B (XPB) protein is a DNA helicase shown to be involved in NER and is also an essential subunit of the Transcription Factor IIH (TFIIH) complex. XPB was found to be a single copy gene in eukaryotes, but found as a tandem duplication in the plant Arabidopsis thaliana, AtXPB1 and AtXPB2. We aimed to investigate whether the XPB in tandem duplication was common within members of the Brassicaceae. We analyzed genomic DNA of species from different tribes of the family and the results indicate that the tandem duplication occurred in Camelineae tribe ancestor, of which A. thaliana belongs, at approximately 8 million years ago. Further experiments were devised to study possible functional roles for the A. thaliana AtXPB paralogs. A non-coincident expression profile of the paralogs was observed in various plant organs, developmental and cell cycle stages. AtXPB2 expression was observed in proliferating cells and clustered with the transcription of other components of the TFIIH such as p44, p52 and XPD/UVH6 along the cell cycle. AtXPB1 gene transcription, on the other hand, was enhanced specifically after UV-B irradiation in leaf trichomes. Altogether, our results reported herein suggest a functional specialization for the AtXPB paralogs: while the AtXPB2 paralog may have a role in cell proliferation and repair as XPB of other eukaryotes, the AtXPB1 paralog is most likely implicated in repair functions in highly specialized A. thaliana cells. (AU)

• Processo FAPESP: 05/56548-8 - Estudo dos componentes do complexo de transcrição e reparo TFIIH, AtXPB1, e AtXPB2 de Arabidopsis thaliana, no reparo de DNA e na transcrição gênica
• Beneficiário: Hana Paula Masuda
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 06/61559-1 - Elementos de transposição e seu impacto no genoma de bactérias e plantas
• Beneficiário: Daniela Kajihara
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 14/15982-6 - Consequências de deficiências de reparo de lesões no genoma
• Beneficiário: Carlos Frederico Martins Menck
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 09/52417-7 - Respostas celulares a lesões no genoma
• Beneficiário: Carlos Frederico Martins Menck
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 98/00004-4 - Organização genômica e expressão do gene araXPB em Arabidopsis thaliana
• Beneficiário: Patrícia Gleydes Morgante
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 02/02655-0 - Análise da estrutura e atividade da região promotora dos genes AtXPB1 e AtXPB2 de Arabidopsis thaliana cv Landsberg ercta
• Beneficiário: Myna Nakabashi
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 98/03925-3 - Metabolismo de DNA em plantas: estabilidade e expressão gênica
• Beneficiário: Marie-Anne Van Sluys
• Modalidade de apoio: Auxílio à Pesquisa - Regular