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Texto completo
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Autor(es):
Mostrar menos - |
Tasima, Lidia J.
[1, 2]
;
Hatakeyama, Daniela M.
[1, 2]
;
Serino-Silva, Caroline
[1, 2]
;
Rodrigues, Caroline F. B.
[1, 2]
;
de Lima, V, Eduardo O.
;
Sant'Anna, Savio S.
[3]
;
Grego, Kathleen F.
[3]
;
de Morais-Zani, Karen
[3, 2]
;
Sanz, Libia
[4]
;
Calvete, Juan J.
[3, 4]
;
Tanaka-Azevedo, Anita M.
[3, 2]
Número total de Autores: 11
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| Afiliação do(s) autor(es): | [1] Inst Butantan, Lab Herpetol, BR-05503000 Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Ciencias Biomed, Interunidades Biotecnol, Inst Pesquisas Tecnol, Inst Butantan, BR-05503000 Sao Paulo - Brazil
[3] de Lima, Eduardo O., V, Inst Butantan, Lab Herpetol, BR-05503000 Sao Paulo - Brazil
[4] CSIC, Lab Venom Evolut & Traslac, Inst Biomed, Jaime Roig 11, Valencia 46010 - Spain
Número total de Afiliações: 4
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| Tipo de documento: |
Artigo Científico
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| Fonte: |
Toxicon;
v. 185,
p. 26-35,
OCT 15 2020.
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| Citações Web of Science: |
0
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| Resumo |
The South American rattlesnake Crotalus durissus spp has a wide geographic distribution in Brazil. Although responsible for only a low proportion of ophidian accidents, it is considered one of the most medically important species of venomous snakes due to the high mortality rate (1.87%). Snake venom is a complex phenotype commonly subjected to individual intraspecific, ontogenetic and geographic variability. Compositional differences in pooled venom used in the immunization process may impact the efficacy of the antivenom. In order to assure standardized high-quality antivenom, the potency of each Brazilian crotalic antivenom batch is determined against the `Brazilian Crotalic Reference Venom' (BCRV). BCRV is produced by Instituto Butantan using venom obtained from the first milking of recently wild-caught C. d. terrificus specimens brought to the Institute. The decrease in the number of snake donations experienced in recent years can become a threat to the production of future batches of BCRV. To evaluate the feasibility of using venom from long-term captive animals in the formulation of BCRV, we have compared the proteomic, biochemical and biological profiles of C. d. terrificus venom pooled from captive specimens (CVP- captive venom pool) and BCRV. Electrophoretic and venomics analyses revealed a very similar venom composition profile, but also certain differences in toxins abundance, with some low abundant protein families found only in BCRV. Enzymatic (L-amino acid oxidase, phospholipase A(2) and proteolytic) and biological (myotoxic and coagulant) activities showed higher values in CVP than in BCRV. CVP also possessed slightly higher lethal effect, although the Instituto Butantan crotalic antivenom showed equivalent potency neutralizing BCRV and CVP. Our results strongly suggest that venom from long-term captive C. d. terrificus might be a valid alternative to generate an immunization mixture of equivalent quality to the currently in use reference venom. (AU) |
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| Processo FAPESP: |
17/01890-0 - Estudo comparativo da composição do veneno das serpentes Crotalus durissus terrificus nascidas em cativeiro no Laboratório de Herpetologia do Instituto Butantan e do veneno crotálico de referência nacional
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| Beneficiário: | Anita Mitico Tanaka-Azevedo |
| Linha de fomento: |
Auxílio à Pesquisa - Regular
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| Processo FAPESP: |
17/16908-2 - Caracterização proteômica e das atividades enzimáticas e fisiopatológicas do veneno das espécies de serpentes que compõem o grupo Bothrops neuwiedi
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| Beneficiário: | Karen de Morais Zani |
| Linha de fomento: |
Auxílio à Pesquisa - Regular
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| Processo FAPESP: |
18/25899-0 - Análise da variabilidade ontogenética do veneno da serpente Bothrops pauloensis
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| Beneficiário: | Lidia Jorge Tasima |
| Linha de fomento: |
Bolsas no Brasil - Mestrado
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| Processo FAPESP: |
17/26533-6 - Estudo qualitativo e quantitativo da enzima L-aminoácido oxidase do veneno de serpentes Crotalus durissus terrificus mantidas por longos períodos em cativeiro e recém-chegadas da natureza
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| Beneficiário: | Eduardo Oliveira Venancio de Lima |
| Linha de fomento: |
Bolsas no Brasil - Iniciação Científica
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| Processo FAPESP: |
18/25786-0 - Estudo dos inibidores de fosfolipase A2 (PLIs) presentes nos plasmas das serpentes peçonhentas e não-peçonhentas e avaliação da atividade neutralizante destes sobre as atividades da fosfolipase A2 (PLA2) dos venenos de serpentes
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| Beneficiário: | Anita Mitico Tanaka-Azevedo |
| Linha de fomento: |
Auxílio à Pesquisa - Regular
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