Extracellular Matrix Proteome Remodeling in Human Glioblastoma and Medulloblastoma

Autor(es):Mostrar menos -

Trombetta-Lima, Marina ^{[1, 2]} ; Rosa-Fernandes, Livia ^[3] ; Angeli, Claudia B. ^[3] ; Moretti, Isabele F. ^[1] ; Franco, Yollanda M. ^[1] ; Mousessian, Adaliana S. ^[1] ; Wakamatsu, Alda ^[4] ; Lerario, Antonio M. ^[5] ; Oba-Shinjo, Sueli M. ^[1] ; Pasqualucci, Carlos A. ^[6] ; Marie, Suely K. N. ^[1] ; Palmisano, Giuseppe ^[3]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med FMUSP, Neurol Dept, Cellular & Mol Biol Lab LIM 15, BR-01246903 Sao Paulo, SP - Brazil

^[2] Univ Groningen, Groningen Res Inst Pharm Grip, Dept Mol Pharmacol, Fac Sci & Engn, NL-9713 AV Groningen - Netherlands

^[3] Univ Sao Paulo, Inst Crencias Biomed ICBUSP, Parasitol Dept, BR-05508000 Sao Paulo, SP - Brazil

^[4] Univ Sao Paulo, Fac Med FMUSP, Pathol Dept, Hepat Pathol Lab LIM 14, BR-01246903 Sao Paulo, SP - Brazil

^[5] Univ Michigan, Div Metab Endocrinol & Diabet, Dept Internal Med, Ann Arbor, MI 48109 - USA

^[6] Univ Sao Paulo, Fac Med FMUSP, Brazilian Aging Brain Study Grp, BR-01246903 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PROTEOME RESEARCH; v. 20, n. 10, p. 4693-4707, OCT 1 2021.
• Citações Web of Science: 0

Resumo

Medulloblastomas (MBs) and glioblastomas (GBMs) are high-incidence central nervous system tumors. Different origin sites and changes in the tissue microenvironment have been associated with the onset and progression. Here, we describe differences between the extracellular matrix (ECM) signatures of these tumors. We compared the proteomic profiles of MB and GBM decellularized tumor samples between each other and their normal decellularized brain site counterparts. Our analysis revealed that 19, 28, and 11 ECM proteins were differentially expressed in MBs, GBMs, and in both MBs and GBMs, respectively. Next, we validated key findings by using a protein tissue array with 53 MB and 55 GBM cases and evaluated the clinical relevance of the identified differentially expressed proteins through their analysis on publicly available datasets, 763 MB samples from the GSE50161 and GSE85217 studies, and 115 GBM samples from RNAseq-TCGA. We report a shift toward a denser fibrillary ECM as well as a clear alteration in the glycoprotein signature, which influences the tumor pathophysiology. (AU)

• Processo FAPESP: 14/06863-3 - Modificações pós-traducionais para o diagnóstico de câncer e doenças parasitárias: abordagens metodológicas e implicações biológicas
• Beneficiário: Giuseppe Palmisano
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 16/14695-9 - O papel do receptor do tipo Toll 4 em células de linhagens tumorais de glioblastoma
• Beneficiário: Isabele Fattori Moretti
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 15/26328-8 - Análise comparativa do papel do gene supressor de tumor RECK na sub-população de células-tronco tumorais de GBM e em precursores neurais normais.
• Beneficiário: Marina Trombetta Lima
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado