Extracellular Matrix Proteome Remodeling in Human Glioblastoma and Medulloblastoma

Author(s):Show less -

Trombetta-Lima, Marina ^{[1, 2]} ; Rosa-Fernandes, Livia ^[3] ; Angeli, Claudia B. ^[3] ; Moretti, Isabele F. ^[1] ; Franco, Yollanda M. ^[1] ; Mousessian, Adaliana S. ^[1] ; Wakamatsu, Alda ^[4] ; Lerario, Antonio M. ^[5] ; Oba-Shinjo, Sueli M. ^[1] ; Pasqualucci, Carlos A. ^[6] ; Marie, Suely K. N. ^[1] ; Palmisano, Giuseppe ^[3]

Affiliation

^[1] Univ Sao Paulo, Fac Med FMUSP, Neurol Dept, Cellular & Mol Biol Lab LIM 15, BR-01246903 Sao Paulo, SP - Brazil

^[2] Univ Groningen, Groningen Res Inst Pharm Grip, Dept Mol Pharmacol, Fac Sci & Engn, NL-9713 AV Groningen - Netherlands

^[3] Univ Sao Paulo, Inst Crencias Biomed ICBUSP, Parasitol Dept, BR-05508000 Sao Paulo, SP - Brazil

^[4] Univ Sao Paulo, Fac Med FMUSP, Pathol Dept, Hepat Pathol Lab LIM 14, BR-01246903 Sao Paulo, SP - Brazil

^[5] Univ Michigan, Div Metab Endocrinol & Diabet, Dept Internal Med, Ann Arbor, MI 48109 - USA

^[6] Univ Sao Paulo, Fac Med FMUSP, Brazilian Aging Brain Study Grp, BR-01246903 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF PROTEOME RESEARCH; v. 20, n. 10, p. 4693-4707, OCT 1 2021.
• Web of Science Citations: 0

Abstract

Medulloblastomas (MBs) and glioblastomas (GBMs) are high-incidence central nervous system tumors. Different origin sites and changes in the tissue microenvironment have been associated with the onset and progression. Here, we describe differences between the extracellular matrix (ECM) signatures of these tumors. We compared the proteomic profiles of MB and GBM decellularized tumor samples between each other and their normal decellularized brain site counterparts. Our analysis revealed that 19, 28, and 11 ECM proteins were differentially expressed in MBs, GBMs, and in both MBs and GBMs, respectively. Next, we validated key findings by using a protein tissue array with 53 MB and 55 GBM cases and evaluated the clinical relevance of the identified differentially expressed proteins through their analysis on publicly available datasets, 763 MB samples from the GSE50161 and GSE85217 studies, and 115 GBM samples from RNAseq-TCGA. We report a shift toward a denser fibrillary ECM as well as a clear alteration in the glycoprotein signature, which influences the tumor pathophysiology. (AU)

• FAPESP's process: 14/06863-3 - Post-translational modifications in cancer and parasite infection diagnosis: methodological approaches and biological implications
• Grantee: Giuseppe Palmisano
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 16/14695-9 - Toll Like Receptor 4 role in glioblastoma cell lineages
• Grantee: Isabele Fattori Moretti
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 15/26328-8 - Comparative analysis of the Reck tumor suppressor gene role in glioblastoma stem cell sub-population and in normal neural stem cells.
• Grantee: Marina Trombetta Lima
• Support Opportunities: Scholarships in Brazil - Post-Doctoral