Post-translational modifications in cancer and parasite infection diagnosis: methodological approaches and biological implications

• Grant number: 14/06863-3
• Support type: Research Grants - Young Investigators Grants
• Duration: February 01, 2015 - September 30, 2019
• Field of knowledge: Biological Sciences - Biochemistry - Chemistry of Macromolecules
• Principal researcher: Giuseppe Palmisano
• Grantee: Giuseppe Palmisano
• Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Assoc. researchers: Cláudio Romero Farias Marinho ; Fernando Augusto Soares ; Vilma Regina Martins
• Associated grant(s): 18/15549-1 - Post-translational modifications in Chagas Disease biological processes and diagnostics: novel methodological approaches and biological applications, AP.JP2; 18/18257-1 - Multi-user equipment approved in grant 14/06863-3: HPLC system configured for analysis of carbohydrates, amino acidis, peptides and glycoproteins, AP.EMU; 16/50356-4 - MYC: a regulator of exosome protein cargo?, AP.R SPRINT
• Associated scholarship(s): 19/01798-2 - Implementation and optimization of computational platforms for expanding the proteome data analysis and OMICs data integration, BP.TT; 18/13283-4 - Discovery of biomarkers of Chagas' disease in urine using mass spectrometry techniques, BP.DR; 18/12349-1 - Post-translation modification in cancer and infectious diseases diagnostics: methodological approaches and biological applications, BP.TT; 16/23689-2 - Proteomic and post-translation modification characterization of Leishmanias L. (V.) braziliensis, L. (L.) amazonensis and L. (L.) chagasi using a shotgun protemics approaches., BP.MS; 15/02866-0 - DISCOVERY AND VERIFICATION OF INTACT GLYCOPEPTIDES AS CANDIDATE PROSTATE CANCER BIOMARKERS USING MASS SPECTROMETRY-BASED PROTEOMICS APPROACHES, BP.PD

Abstract

An innovative research program will be established aiming at studying protein post-translational modifications (PTMs), such as phosphorylation, glycosylation and oxidation modulation in pathological conditions such as cancer metastasis and parasite infection diagnosis. The research will be articulated on three lines: 1) Optimize and develop mass spectrometry-based enrichment/analysis methods for PTMs studies in different biological systems; 2) Develop a strategy for the recovery and analysis of secreted modified proteins and peptides originating from interstitial fluid from dissected prostate cancers and surrounding healthy tissues; 3) Establish a mass spectrometry-based method to detect biomarkers of parasite infection in biofluids in order to develop a point-of-care diagnostic platform capable of detecting these diseases at an early stage. (AU)

Scientific publications

(37)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MULE, SIMON NGAO; COSTA-MARTINS, ANDRE GUILHERME; ROSA-FERNANDES, LIVIA; DE OLIVEIRA, GILBERTO SANTOS; RODRIGUES, CARLA MONADELI F.; QUINA, DANIEL; ROSEIN, GRAZIELLA E.; GERALDES TEIXEIRA, MARTA MARIA; PALMISANO, GIUSEPPE. PhyloQuant approach provides insights into Trypanosoma cruzi evolution using a systems-wide mass spectrometry-based quantitative protein profile. COMMUNICATIONS BIOLOGY, v. 4, n. 1, MAR 11 2021. (18/13283-4, 17/04032-5, 14/06863-3, 18/15549-1, 18/18257-1, 14/25494-9)

GAVINHO, BRUNO; SABATKE, BRUNA; FEIJOLI, VERONICA; ROSSI, IZADORA VOLPATO; DA SILVA, JANAINA MACEDO; EVANS-OSSES, INGRID; PALMISANO, GIUSEPPE; LANGE, SIGRUN; RAMIREZ, MARCEL IVAN. Peptidylarginine Deiminase Inhibition Abolishes the Production of Large Extracellular Vesicles FromGiardia intestinalis, Affecting Host-Pathogen Interactions by Hindering Adhesion to Host Cells. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 10, SEP 23 2020. (18/18257-1, 14/06863-3, 18/15549-1)

ROSA-FERNANDES, LIVIA; BARBOSA, RAQUEL HORA; DOS SANTOS, MARIA LUIZA B.; ANGELI, CLAUDIA B.; SILVA, THIAGO P.; MELO, ROSSANA C. N.; DE OLIVEIRA, GILBERTO SANTOS; LEMOS, BERNARDO; VAN EYK, JENNIFER E.; LARSEN, MARTIN R.; et al. Cellular Imprinting Proteomics Assay: A Novel Method for Detection of Neural and Ocular Disorders Applied to Congenital Zika Virus Syndrome. JOURNAL OF PROTEOME RESEARCH, v. 19, n. 11, p. 4496-4515, NOV 6 2020. (18/18257-1, 14/06863-3, 18/15549-1)

MOREIRA, RAFAEL J.; CASTRO, ERIQUE; OLIVEIRA, TIAGO E.; BELCHIOR, THIAGO; PEIXOTO, ALBERT S.; CHAVES-FILHO, ADRIANO B.; MORENO, MAYARA F.; LIMA, JANAYNA D.; YOSHINAGA, MARCOS; MIYAMOTO, SAYURI; et al. Lipoatrophy-Associated Insulin Resistance and Hepatic Steatosis are Attenuated by Intake of Diet Rich in Omega 3 Fatty Acids. MOLECULAR NUTRITION & FOOD RESEARCH, v. 64, n. 7, FEB 2020. (16/04000-3, 17/17943-6, 13/07937-8, 15/19530-5, 14/06863-3, 18/15549-1, 13/07467-1)

DE CASTRO, PATRICIA ALVES; COLABARDINI, ANA CRISTINA; MANFIOLLI, ADRIANA OLIVEIRA; CHIARATTO, JESSICA; SILVA, LILIAN PEREIRA; MATTOS, ELICIANE CEVOLANI; PALMISANO, GIUSEPPE; ALMEIDA, FAUSTO; PERSINOTI, GABRIELA FELIX; ANNICK RIES, LAURE NICOLAS; et al. Aspergillus fumigatus calcium-responsive transcription factors regulate cell wall architecture promoting stress tolerance, virulence and caspofungin resistance. PLOS GENETICS, v. 15, n. 12, DEC 2019. (17/07536-4, 16/21392-2, 14/06863-3, 16/12948-7, 16/07870-9, 16/22062-6, 14/04783-2, 17/19288-5)

RODRIGUES, MARIANE A. D.; PIMENTA, MARCELA V.; COSTA, IRIS M.; ZENATTI, PRISCILA P.; MIGITA, NATACHA A.; YUNES, JOSE A.; RANGEL-YAGUI, CARLOTA O.; DE SA, MATHEUS M.; PESSOA, ADALBERTO; COSTA-SILVA, TALES A.; et al. Influence of lysosomal protease sensitivity in the immunogenicity of the antitumor biopharmaceutical asparaginase. Biochemical Pharmacology, v. 182, DEC 2020. (14/06863-3, 18/18257-1, 13/08617-7, 16/25896-5, 15/07749-2, 18/15104-0, 13/08139-8, 18/15549-1)

LIMA, GUILHERME MEIRA; EFFER, BRIAN; BIASOTO, HENRIQUE PELLIN; FEIJOLI, VERONICA; PESSOA, ADALBERTO; PALMISANO, GIUSEPPE; MONTEIRO, GISELE. Glycosylation of L-asparaginase from E. coli through yeast expression and site-directed mutagenesis. Biochemical Engineering Journal, v. 156, APR 15 2020. (15/07749-2, 13/08617-7, 14/06863-3, 18/18257-1, 18/15549-1, 16/15787-4)

DE OLIVEIRA, GILBERTO SANTOS; KAWAHARA, REBECA; ROSA-FERNANDES, LIVIA; AVILA, CARLA C.; LARSEN, MARTIN R.; PEREIRA ALVES, JOAO MARCELO; PALMISANO, GIUSEPPE. Novel DNA coding regions and protein arginylation reveal unexplored T. cruzi proteome and PTMs. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, v. 418, n. SI, p. 51-66, JUL 2017. (15/02866-0, 14/06863-3)

HUANG, HONGGANG; PETERSEN, MARTIN HAAR; IBANEZ-VEA, MARIA; LASSEN, PERNILLE S.; LARSEN, MARTIN R.; PALMISANO, GIUSEPPE. Simultaneous Enrichment of Cysteine-containing Peptides and Phosphopeptides Using a Cysteine-specific Phosphonate Adaptable Tag (CysPAT) in Combination with titanium dioxide (TiO2) Chromatography. MOLECULAR & CELLULAR PROTEOMICS, v. 15, n. 10, p. 3282-3296, OCT 2016. (14/06863-3)

FERNANDES, LIVIA ROSA; BASSI STERN, ANA CAROLINA; CAVAGLIERI, RITA DE CASSIA; SOUSA NOGUEIRA, FABIO CESAR; DOMONT, GILBERTO; PALMISANO, GIUSEPPE; BYDLOWSKI, SERGIO PAULO. 7-Ketocholesterol overcomes drug resistance in chronic myeloid leukemia cell lines beyond MDRI mechanism. JOURNAL OF PROTEOMICS, v. 151, n. SI, p. 12-23, JAN 16 2017. (14/06863-3)

CIREGIA, FEDERICA; URBANI, ANDREA; PALMISANO, GIUSEPPE. Extracellular Vesicles in Brain Tumors and Neurodegenerative Diseases. FRONTIERS IN MOLECULAR NEUROSCIENCE, v. 10, AUG 31 2017. (14/06863-3, 16/50356-4)

RAMIREZ, MARCEL I.; AMORIM, MARIA G.; GADELHA, CATARINA; MILIC, IVANA; WELSH, JOSHUA A.; FREITAS, VANESSA M.; NAWAZ, MUHAMMAD; AKBAR, NAVEED; COUCH, YVONNE; MAKIN, LAURA; et al. Technical challenges of working with extracellular vesicles. NANOSCALE, v. 10, n. 3, p. 881-906, JAN 21 2018. (14/06863-3, 15/50257-3, 12/24574-3, 14/26897-0)

ROSA-FERNANDES, LIVIA; ROCHA, VICTORIA BOMBARDA; CARREGARI, VICTOR CORASOLLA; URBANI, ANDREA; PALMISANO, GIUSEPPE. A Perspective on Extracellular Vesicles Proteomics. FRONTIERS IN CHEMISTRY, v. 5, NOV 21 2017. (14/06863-3)

ROSA-FERNANDES, LIVIA; MASELLI, LUCIANA M. P.; MAEDA, NAIR Y.; PALMISANO, GIUSEPPE; BYDLOWSKI, SERGIO P.. Outside-in, inside-out: Proteomic analysis of endothelial stress mediated by 7-ketocholesterol. Chemistry and Physics of Lipids, v. 207, n. B, p. 231-238, OCT 2017. (14/06863-3)

LATROFA, MARIA STEFANIA; PALMISANO, GIUSEPPE; ANNOSCIA, GIADA; PIERRI, CIRO LEONARDO; CHANDRASHEKAR, RAMASWAMY; OTRANTO, DOMENICO. Major antigen and paramyosin proteins as candidate biomarkers for serodiagnosis of canine infection by zoonotic Onchocerca lupi. PLoS Neglected Tropical Diseases, v. 15, n. 2, FEB 2021. (17/04032-5, 18/18257-1, 14/06863-3, 18/15549-1)

FUZITA, FELIPE J.; PIMENTA, DANIEL C.; PALMISANO, GIUSEPPE; TERRA, WALTER R.; FERREIRA, CLELIA. Detergent-resistant domains in Spodoptera frugiperda midgut microvillar membranes and their relation to microapocrine secretion. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, v. 235, p. 8-18, SEP 2019. (17/08103-4, 14/14183-2, 14/06863-3)

PEREIRA, ADRIANA S. A.; AMARAL, MURILO S.; VASCONCELOS, ELTON J. R.; PIRES, DAVID S.; ASIF, HUMA; DASILVA, LUCAS F.; MORALES-VICENTE, DAVID A.; CARNEIRO, VITOR C.; ANGELI, CLAUDIA B.; PALMISANO, GIUSEPPE; et al. Inhibition of histone methyltransferase EZH2 in Schistosoma mansoni in vitro by GSK343 reduces egg laying and decreases the expression of genes implicated in DNA replication and noncoding RNA metabolism. PLoS Neglected Tropical Diseases, v. 12, n. 10, OCT 2018. (16/10046-6, 14/24560-8, 14/06863-3)

GOMES, LAURA CORDEIRO; SANSON, MARINA CORDEIRO GOMES; BRAININ, PHILIP; DE MELO, MARIA DA CONCEICAO VIEIRA; DE SOUZA, RODRIGO MEDEIROS; MAZARO, JANAINA; LIMA, KARINE OLIVEIRA; RESENDE, JUNIA SILVA; VIEIRA, ISABELLE VICTORIA MARTINS; MESQUITA, EDINILSON DA SILVA; et al. Levels of hepatitis B antibody titers are affected by age and doses gap time in children from a high endemic area of the western Amazon. PLoS One, v. 16, n. 7, 2021. (18/20468-0, 18/18257-1, 18/15549-1, 15/26722-8, 14/06863-3)

FUZITA, FELIPE J.; PALMISANO, GIUSEPPE; PIMENTA, DANIEL C.; TERRA, WALTER R.; FERREIRA, CLELIA. A proteomic approach to identify digestive enzymes, their exocytic and microapocrine secretory routes and their compartmentalization in the midgut of Spodoptera frugiperda. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, v. 257, JAN 2022. (14/06863-3, 17/08103-4, 14/14183-2)

MISERANI MAGALHAES, RUBENS DANIEL; MATTOS, ELICIANE CEVOLANI; ROZANSKI, ANDREI; FAVORETTO GALANTE, PEDRO ALEXANDRE; PALMISANO, GIUSEPPE; CRUZ, ANGELA KAYSEL; COLLI, WALTER; CAMARGO, ANAMARIA ARANHA; MANSO ALVES, MARIA JULIA. Global changes in nitration levels and DNA binding profile of Trypanosoma cruzi histones induced by incubation with host extracellular matrix. PLoS Neglected Tropical Diseases, v. 14, n. 5, MAY 2020. (14/10046-0, 13/16002-2, 18/15549-1, 18/18257-1, 14/25494-9, 14/06863-3)

TROMBETTA-LIMA, MARINA; ROSA-FERNANDES, LIVIA; ANGELI, CLAUDIA B.; MORETTI, ISABELE F.; FRANCO, YOLLANDA M.; MOUSESSIAN, ADALIANA S.; WAKAMATSU, ALDA; LERARIO, ANTONIO M.; OBA-SHINJO, SUELI M.; PASQUALUCCI, CARLOS A.; et al. Extracellular Matrix Proteome Remodeling in Human Glioblastoma and Medulloblastoma. JOURNAL OF PROTEOME RESEARCH, v. 20, n. 10, p. 4693-4707, OCT 1 2021. (14/06863-3, 16/14695-9, 15/26328-8)

KAWAHARA, REBECA; RECUERO, SAULO; NOGUEIRA, FABIO C. S.; DOMONT, GILBERTO B.; LEITE, KATIA R. M.; SROUGI, MIGUEL; THAYSEN-ANDERSEN, MORTEN; PALMISANO, GIUSEPPE. Tissue Proteome Signatures Associated with Five Grades of Prostate Cancer and Benign Prostatic Hyperplasia. PROTEOMICS, v. 19, n. 21-22, SI, NOV 2019. (18/15549-1, 15/02866-0, 18/18257-1, 17/03010-8, 14/06863-3)

MARTINS, LARISSA ALMEIDA; PALMISANO, GIUSEPPE; CORTEZ, MAURO; KAWAHARA, REBECA; DE FREITAS BALANCO, JOSE MARIO; FUJITA, ANDRE; ALONSO, BEATRIZ IGLESIAS; BARROS-BATTESTI, DARCI MORAES; BRAZ, GLORIA REGINA CARDOSO; TIRLONI, LUCAS; et al. The intracellular bacterium Rickettsia rickettsii exerts an inhibitory effect on the apoptosis of tick cells. PARASITES & VECTORS, v. 13, n. 1, DEC 1 2020. (12/24105-3, 18/15549-1, 18/18257-1, 14/11513-1, 14/06863-3, 14/05855-7, 13/26450-2)

KISCHKEL, BRENDA; BONICHE-ALFARO, CAMILA; MENEZES, ISABELA DE GODOY; ROSSI, SUELEN ANDREIA; ANGELI, CLAUDIA BLANES; DE ALMEIDA, SANDRO ROGERIO; PALMISANO, GIUSEPPE; LOPES-BEZERRA, LEILA; NOSANCHUK, JOSHUA D.; TABORDA, CARLOS PELLESCHI. Immunoproteomic and Immunopeptidomic Analyses of Histoplasma capsulatum Reveal Promiscuous and Conserved Epitopes Among Fungi With Vaccine Potential. FRONTIERS IN IMMUNOLOGY, v. 12, NOV 22 2021. (18/18257-1, 16/08730-6, 14/06863-3, 17/25780-0, 18/26402-1, 18/15549-1)

KAWAHARA, REBECA; RECUERO, SAULO; SROUGI, MIGUEL; LEITE, KATIA R. M.; THAYSEN-ANDERSEN, MORTEN; PALMISANO, GIUSEPPE. The Complexity and Dynamics of the Tissue Glycoproteome Associated With Prostate Cancer Progression. MOLECULAR & CELLULAR PROTEOMICS, v. 20, 2021. (15/02866-0, 18/18257-1, 14/06863-3, 18/15549-1, 17/03010-8)

CAPELLI-PEIXOTO, JANAINA; MULE, SIMON NGAO; TANO, FABIA TOMIE; PALMISANO, GIUSEPPE; STOLF, BEATRIZ SIMONSEN. Proteomics and Leishmaniasis: Potential Clinical Applications. PROTEOMICS CLINICAL APPLICATIONS, v. 13, n. 6, AUG 2019. (17/26197-6, 17/04287-3, 14/26777-4, 14/06863-3, 18/18257-1, 18/15549-1, 17/04032-5)

ROSA-FERNANDES, LIVIA; CUGOLA, FERNANDA RODRIGUES; RUSSO, FABIELE BALDINO; KAWAHARA, REBECA; DE MELO FREIRE, CAIO CESAR; CORREA LEITE, PAULO EMLIO; BASSI STERN, ANA CAROLINA; ANGELI, CLAUDIA BLANES; LEAL DE OLIVEIRA, DANIELLE BRUNA; MELO, STELLA REZENDE; et al. Zika Virus Impairs Neurogenesis and Synaptogenesis Pathways in Human Neural Stem Cells and Neurons. FRONTIERS IN CELLULAR NEUROSCIENCE, v. 13, MAR 15 2019. (16/02978-6, 14/06863-3, 13/08844-3, 18/16748-8, 11/20683-0, 15/02866-0)

CHATTERJEE, SAYANTANI; LEE, LING Y.; KAWAHARA, REBECA; ABRAHAMS, JODIE L.; ADAMCZYK, BARBARA; ANUGRAHAM, MERRINA; ASHWOOD, CHRISTOPHER; SUMER-BAYRAKTAR, ZEYNEP; BRIGGS, MATTHEW T.; CHIK, JENNY H. L.; et al. Protein Paucimannosylation Is an Enriched N-Glycosylation Signature of Human Cancers. PROTEOMICS, v. 19, n. 21-22, SI, NOV 2019. (15/02866-0, 17/03010-8, 14/06863-3, 18/15549-1)

DE OLIVEIRA, GILBERTO SANTOS; KAWAHARA, REBECA; ROSA-FERNANDES, LIVIA; MULE, SIMON NGAO; AVILA, CARLA CRISTI; TEIXEIRA, MARTA M. G.; LARSEN, MARTIN R.; PALMISANO, GIUSEPPE. Development of a Trypanosoma cruzi strain typing assay using MS2 peptide spectral libraries (Tc-STAMS2). PLoS Neglected Tropical Diseases, v. 12, n. 4, APR 2018. (14/06863-3, 15/02866-0)

BOYSEN, ANDERS; PALMISANO, GIUSEPPE; KROGH, THOGER JENSEN; DUGGIN, IAIN G.; LARSEN, MARTIN R.; MOLLER-JENSEN, JAKOB. A novel mass spectrometric strategy ``BEMAP{''} reveals Extensive O-linked protein glycosylation in Enterotoxigenic Escherichia coli. SCIENTIFIC REPORTS, v. 6, AUG 26 2016. (14/06863-3)

PIMENTEL, ANDRE C.; FUZITA, FELIPE J.; PALMISANO, GIUSEPPE; FERREIRA, CELIA; TERRA, WALTER R.. Role of cathepsins D in the midgut of Dysdercus peruvianus. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, v. 204, p. 45-52, FEB 2017. (14/06863-3)

AVILA, C. C.; ALMEIDA, F. G.; PALMISANO, G.. Direct identification of trypanosomatids by matrix-assisted laser desorption ionization-time of flight mass spectrometry (DIT MALDI-TOF MS). Journal of Mass Spectrometry, v. 51, n. 8, p. 549-557, AUG 2016. (14/06863-3)

KAWAHARA, REBECA; SAAD, JOYCE; ANGELI, CLAUDIA BLANES; PALMISANO, GIUSEPPE. Site-specific characterization of N-linked glycosylation in human urinary glycoproteins and endogenous glycopeptides. GLYCOCONJUGATE JOURNAL, v. 33, n. 6, p. 937-951, DEC 2016. (15/02866-0, 14/06863-3)

CARREGARI, VICTOR CORASSOLLA; ROSA-FERNANDES, LIVIA; BALDASSO, PAULO; BYDLOWSKI, SERGIO PAULO; MARANGONI, SERGIO; LARSEN, MARTIN R.; PALMISANO, GIUSEPPE. Snake Venom Extracellular vesicles (SVEVs) reveal wide molecular and functional proteome diversity. SCIENTIFIC REPORTS, v. 8, AUG 13 2018. (14/06863-3)

AVILA, CARLA CRISTI; MULE, SIMON NGAO; ROSA-FERNANDES, LIVIA; VINER, ROSA; BARISON, MARIA JULIA; COSTA-MARTINS, ANDRE GUILLHERME; DE OLIVEIRA, GILBERTO SANTOS; GERALDES TEIXEIRA, MARTA MARIA; FARIAS MARINHO, CLAUDIO ROMERO; SILBER, ARIEL MARIANO; et al. Proteome-Wide Analysis of Trypanosoma cruzi Exponential and Stationary Growth Phases Reveals a Subcellular Compartment-Specific Regulation. GENES, v. 9, n. 8, AUG 2018. (16/06034-2, 14/06863-3)

MANSO ALVES, MARIA JULIA; KAWAHARA, REBECA; VINER, ROSA; COLLI, WALTER; MATTOS, ELICIANE CEVOLANI; THAYSEN-ANDERSEN, MORTEN; LARSEN, MARTIN ROSSEL; PALMISANO, GIUSEPPE. Comprehensive glycoprofiling of the epimastigote and trypomastigote stages of Trypanosoma cruzi. JOURNAL OF PROTEOMICS, v. 151, n. SI, p. 182-192, JAN 16 2017. (13/16478-7, 14/25494-9, 14/06863-3)

COUTINHO, JOAO V. P.; ROSA-FERNANDES, LIVIA; MULE, SIMON NGAO; DE OLIVEIRA, GILBERTO SANTOS; MANCHOLA, NUBIA CAROLINA; SANTIAGO, VERONICA FEIJOLI; COLLI, WALTER; WRENGER, CARSTEN; MANSO ALVES, MARIA JULIA; PALMISANO, GIUSEPPE. The thermal proteome stability profile of Trypanosoma cruzi in epimastigote and trypomastigote life stages. JOURNAL OF PROTEOMICS, v. 248, SEP 30 2021. (18/13283-4, 18/15549-1, 14/06863-3, 17/03966-4, 15/26722-8, 17/19854-0, 17/04032-5, 18/18257-1)