Disturbances of the transfer of cholesterol to high-density lipoprotein (HDL) in patients with peripheral artery disease with or without type 2 diabetes mellitus

Autor(es)

de Macedo Ribeiro, Flavio Roberto Cavalleiro ^[1] ; Ribeiro, Carolina Heitmann Mares Azevedo ^[2] ; Tavoni, Thauany Martins ^{[3, 1]} ; dos Santos Sarges, Erica ^[2] ; Freitas, Fatima Rodrigues ^[1] ; Stolf, Noedir Antonio Groppo ^[1] ; Kalil Filho, Roberto ^[1] ; Maranhao, Raul Cavalcante ^{[3, 1]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Coracao InCor, Hosp Clin HCFMUSP, Fac Med, Sao Paulo - Brazil

^[2] Univ Fed Para, Inst Ciencias Saude, Dept Ciencias Farmaceut, Belem, Para - Brazil

^[3] Univ Sao Paulo, Fac Ciencias Farmaceut, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: VASCULAR MEDICINE; v. 26, n. 6, p. 602-607, DEC 2021.
• Citações Web of Science: 0

Resumo

Introduction: Low high-density lipoprotein (HDL)-cholesterol is frequent in patients with peripheral artery disease (PAD) and also in type 2 diabetes mellitus (T2DM), the major risk factor for PAD. The transfer of cholesterol from the other lipoproteins to HDL is an important aspect of HDL metabolism and function, and may contribute to atherogenic mechanisms that lead to PAD development. Objective: The aim of this study was to investigate the status of cholesterol transfers in patients with PAD without or with T2DM. Methods: Patients with PAD (n = 19), with PAD and T2DM (PAD + DM, n = 19), and healthy controls (n = 20), all paired for age, gender, and BMI were studied. Transfer of both forms of cholesterol, unesterified (UC) and esterified (EC), was performed by incubating plasma with a donor nanoemulsion containing radioactive UC and EC, followed by chemical precipitation and HDL radioactive counting. Results: Low-density lipoprotein (LDL)-cholesterol and triglycerides were similar in the three groups. Compared to controls, HDL-C was lower in PAD + DM (p < 0.05), but not in PAD. Transfer of UC was lower in PAD + DM than in PAD and controls (4.18 +/- 1.17%, 5.13 +/- 1.44%, 6.59 +/- 1.25%, respectively, p < 0.001). EC transfer tended to be lower in PAD + DM than in controls (2.96 +/- 0.60 vs 4.12 +/- 0.89%, p = 0.05). Concentrations of cholesteryl ester transfer protein (CETP) and lecithin-cholesterol acyltransferase (LCAT), both involved in HDL metabolism, were not different among the three groups. Conclusion: Deficient cholesterol transfer to HDL may play a role in PAD pathogenesis. Since UC transfer to HDL was lower in PAD + DM compared to PAD alone, it is possible that defective HDL metabolism may contribute to the higher PAD incidence in patients with T2DM.Keywords (AU)

• Processo FAPESP: 14/03742-0 - Projeto temático em medicina translacional: nanopartículas que se ligam a receptores de lipoproteínas no tratamento da aterosclerose, do infarto agudo de miocárdio, do pós-transplante de coração, do câncer e da endometriose
• Beneficiário: Raul Cavalcante Maranhao
• Modalidade de apoio: Auxílio à Pesquisa - Temático