Plasma advanced glycation end products and soluble receptor for advanced glycation end products as indicators of sterol content in human carotid atherosclerotic plaques

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Pinto, Raphael S. ; Ferreira, Guilherme S. ; Silvestre, Gina Camillo R. ; Santana, Monique de Fatima M. ; Nunes, Valeria S. ; Ledesma, Lucas ; Pinto, Paula R. ; de Assis, Sayonara Ivana S. ; Machado, Ubiratan F. ; da Silva, Erasmo S. ; Passarelli, Marisa

• Tipo de documento: Artigo Científico
• Fonte: Diabetes & Vascular Disease Research; v. 19, n. 1, p. 9-pg., 2022-01-01.

Resumo

Advanced glycation end products (AGEs) are independently related to cardiovascular disease (CVD) and favor cholesterol and oxysterol accumulation in macrophage foam cells. Soluble RAGE (sRAGE) impairs cellular AGE signaling alleviating the deleterious effects of AGE in atherogenesis. The association between plasma AGEs and sRAGE with the content of cholesterol, markers of cholesterol synthesis and absorption, and oxysterols in atherosclerotic plaques was evaluated in subjects undergoing carotid endarterectomy. Plasma and carotid plaques were obtained from symptomatic (n = 23) and asymptomatic subjects (n = 40). Lipids from plaques were extracted and sterols (oxysterols, cholesterol, desmosterol, lathosterol, sitosterol, and campesterol) were determined by using gas chromatography/mass spectrometry. Plasma total AGEs and pentosidine were measured by using fluorimetry and sRAGE by using ELISA. In symptomatic subjects ' atherosclerotic plaques, an increased amount of cholesterol (3x) and oxysterols [7 alpha-hydroxycholesterol (1.4x); 7 beta-hydroxycholesterol (1.2x); 25-hydroxycholesterol (1.3x); 24-hydroxycholesterol (2.7x), and 27-hydroxycholesterol, (1.15x)], with exception to 7 ketocholesterol, were found in comparison to asymptomatic individuals. Plasma total AGEs and pentosidine significantly and positively correlated to sterols accumulated in the atherosclerotic lesion, including cholesterol, desmosterol, campesterol, sitosterol, and oxysterols. On the other hand, sRAGE inversely correlated to total AGEs and pentosidine in plasma, and with major species of oxysterols, cholesterol, and markers of cholesterol synthesis and absorption in the atherosclerotic lesion. In multiple regression analyses, it was observed a significant inverse correlation between sRAGE and 24-hydroxycholesterol and desmosterol, and a positive significant correlation between pentosidine and 24-hydroxycholesterol, 27-hydroxycholesterol, and campesterol. In conclusion, the plasma concentration of AGEs and sRAGE is a tool to predict the accumulation of sterols in atherosclerotic lesions in symptomatic and asymptomatic individuals, helping to prevent and improve the management of acute cardiovascular complications. (AU)

• Processo FAPESP: 18/00172-0 - Produtos de glicação avançada, estresse de retículo endoplasmático e modulação do transporte reverso de colesterol em artérias humanas com diferentes graus de Aterosclerose
• Beneficiário: Raphael de Souza Pinto
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/15603-0 - Desvendando mecanismos envolvidos no controle glicêmico e nas complicações crônicas do Diabetes mellitus: contribuições à saúde humana
• Beneficiário: Ubiratan Fabres Machado
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 15/21072-5 - Influência do controle glicêmico ou do estágio da nefropatia sobre o transporte reverso de colesterol no Diabete melito: papel da memória metabólica induzida pela albumina modificada por glicação avançada
• Beneficiário: Marisa Passarelli
• Modalidade de apoio: Auxílio à Pesquisa - Regular