Epigenomic integrative analysis pinpoint master regulator transcription factors associated with tumorigenesis in squamous cell carcinoma of oral tongue

Autor(es)

Larissa Miyuki Okano ^[1] ; Lívia Maria Maciel da Fonseca ^[2] ; Isabela Dias Erthal ^[3] ; Tathiane Maistro Malta ^[4]

Afiliação do(s) autor(es)

^[1] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto - Brasil

^[2] Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto - Brasil

^[3] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto - Brasil

^[4] Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto - Brasil

• Tipo de documento: Artigo Científico
• Fonte: GENETICS AND MOLECULAR BIOLOGY; v. 46, n. 2 2023-06-19.

Resumo

Abstract Head and Neck Cancer (HNC) is a heterogeneous group of cancers, which includes cancers arising in the oral cavity, nasopharynx, oropharynx, hypopharynx, and larynx. Epidemiological studies have revealed that several factors such as tobacco and alcohol use, exposure to environmental pollutants, viral infection, and genetic factors are risk factors for developing HNC. The squamous cell carcinoma of oral tongue (SCCOT), which is significantly more aggressive than the other forms of oral squamous cell carcinoma, presents a propensity for rapid local invasion and spread, and a high recurrence rate. Dysregulation in the epigenetic machinery of cancer cells might help uncover the mechanisms of SCOOT tumorigenesis. Here, we used DNA methylation changes to identify cancer-specific enhancers that were enriched for specific transcription factor binding sites (TFBS), and potential master regulator transcription factors (MRTF) associated with SCCOT. We identified the activation of MRTFs associated with increased invasiveness, metastasis, epithelial-to-mesenchymal transition, poor prognosis, and stemness. On the other hand, we found the downregulation of MRTFs associated with tumor suppression. The identified MRTFs should be further investigated to clarify their role in oral cancer tumorigenesis and for their potential use as biological markers. (AU)

• Processo FAPESP: 21/08190-0 - Mapeamento de assinaturas epigenômicas associadas com mutação IDH
• Beneficiário: Isabela Dias Erthal
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 18/00583-0 - Análise epigenômica integrativa de gliomas: definindo regiões regulatórias associadas ao stemness e ao fenótipo hipermetilador de tumores com mutações nos genes IDH1/2
• Beneficiário: Tathiane Maistro Malta Pereira
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 19/14928-1 - Análise epigenômica integrativa de gliomas - definindo regiões regulatórias associadas ao "stemness" e ao fenótipo hipermetilador de tumores com mutações nos genes IDH1/2
• Beneficiário: Tathiane Maistro Malta Pereira
• Modalidade de apoio: Bolsas no Brasil - Jovens Pesquisadores