Brazilian Ginseng extraction via LPSE and SFE: Global yields, extraction kinetics, chemical composition and antioxidant activity

Autor(es)

Leal, Patricia F. ^[1] ; Kfouri, Marina B. ^[1] ; Alexandre, Fabio C. ^[1] ; Fagundes, Fabio H. R. ^[2] ; Prado, Juliana M. ^[1] ; Toyama, Marcos H. ^[2] ; Meireles, M. Angela A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Sch Food Engn, DEA, LASEFI, FEA, BR-13083862 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, IB, LAQUIP, BR-13083862 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF SUPERCRITICAL FLUIDS; v. 54, n. 1, p. 38-45, JUL 2010.
• Citações Web of Science: 32

Resumo

Brazilian Ginseng extracts of two species, Pfaffia paniculata and Pfaffia glomerata, were obtained by supercritical fluid extraction (SFE) with CO(2) and by low-pressure solvent extraction (LPSE) with methanol, hexane and ethanol. The SFE assays were conducted at pressures of 100, 200 and 300 bar, and temperatures of 30 and 50 degrees C. The qualitative chemical compositions of the extracts were determined by thin layer chromatography (TLC). One of the active principles of interest from P. glomerata extract, beta-ecdysone, was identified and quantified by HPLC. The antioxidant activities of Brazilian Ginseng extracts were determined by the coupled reaction of linolenic acid and beta-carotene. For P. paniculata, the highest SFE yield was obtained at 200 bar/50 degrees C (0.22%, dry basis d.b.), while the best extraction condition for P. glomerata was obtained at 200 bar/30 degrees C (0.18%, d.b.). The higher extract yields obtained by LPSE were 2.0% and 5.8% (w/w, d.b.) for P. paniculata and P. glomerata, respectively, both obtained with methanol as extraction solvent. From the overall extraction curve of P. glomerata, it was possible to obtain the kinetic parameters of extraction; the duration of the CER (constant extraction rate) period was determined as 134 min. The TLC plates showed the possible presence of flavonoids in the ethanolic extract for both Pfaffia species. The antioxidant activity analysis detected that LPSE extracts had higher activity than SFE extracts. (C) 2010 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 04/09310-3 - Estudo comparativo entre os custos de manufatura e as propriedades funcionais de óleos voláteis obtidos por extração supercrítica e destilação por arraste a vapor
• Beneficiário: Patricia Franco Leal Gestic
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 99/01962-1 - Tecnologia supercrítica aplicada ao processamento de óleos essenciais, óleos vegetais, corantes, estévia, amido, celulose, etc.
• Beneficiário: Maria Angela de Almeida Meireles Petenate
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 07/03817-7 - Estudo de aumento de escala de processo de extração supercrítica em leito fixo
• Beneficiário: Juliana Martin Do Prado
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 00/08006-8 - Estudo da acao antioxidante de extratos de gengibre, curcuma e alecrim obtidos por extracao supercritica com co2 e co-solventes.
• Beneficiário: Patricia Franco Leal Gestic
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica