Steroidogenic Factor 1 Overexpression and Gene Amplification Are More Frequent in Adrenocortical Tumors from Children than from Adults

Autor(es):Mostrar menos -

Almeida, Madson Q. ^[1] ; Soares, Ibere Cauduro ^[2] ; Ribeiro, Tamaya C. ^[1] ; Fragoso, Maria Candida B. V. ^[1] ; Marins, Lidiane V. ^[2] ; Wakamatsu, Alda ^[2] ; Ressio, Rodrigo A. ^[2] ; Nishi, Mirian Y. ^[1] ; Jorge, Alexander A. L. ^[1] ; Lerario, Antonio M. ^[1] ; Alves, Venancio A. F. ^[2] ; Mendonca, Berenice B. ^[1] ; Latronico, Ana Claudia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Hosp Clin, Fac Med, Lab Hormonios & Genet Mol, Lab Invest Med, Unidade Endocrinol Desenvolvimento, Disciplina End, BR-05403900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Hosp Clin, Fac Med, Div Anat Patol, Lab Patol Hepat LIM 14, BR-05403900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM; v. 95, n. 3, p. 1458-1462, MAR 2010.
• Citações Web of Science: 48

Resumo

Background: Steroidogenic factor 1 (SF-1) is a key determinant of endocrine development and function of adrenal cortex. SF-1 overexpression and gene amplification were previously demonstrated in a small group of pediatric adrenocortical tumors. Objective: Our objective was to determine the frequency of SF-1 protein expression and gene amplification in a large cohort of pediatric and adult adrenocortical tumors. Patients: SF-1 protein expression was assessed in a cohort of 103 adrenocortical tumors from 36 children and 67 adults, whereas gene amplification was studied in 38 adrenocortical tumors ( 17 from children). Methods: Tissue microarray, multiplex ligation-dependent probe amplification, and quantitative real-time PCR were used. Results: Astrong nuclear SF-1 expression was detected by tissue microarray in 56% (20 of 36) and 19% (13 of 67) of the pediatric and adult adrenocortical tumors, respectively (P = 0.0004). Increased SF-1 copy number was identified in 47% (eight of 17) and 10% (two of 21) of the pediatric and adult adrenocortical tumors, respectively (P = 0.02). All adrenocortical tumors with SF-1 gene amplification showed a strong SF-1 staining, whereas most of the tumors (61%) without SF-1 amplification displayed a weak or negative staining (P = 0.0008). Interestingly, a strong SF-1 staining was identified in five (29%) pediatric adrenocortical tumors without SF-1 amplification. The frequency of SF-1 overexpression and gene amplification was similar in adrenocortical adenomas and carcinomas. Conclusion: We demonstrated a higher frequency of SF-1 overexpression and gene amplification in pediatric than in adult adrenocortical tumors, suggesting an important role of SF-1 in pediatric adrenocortical tumorigenesis. (J Clin Endocrinol Metab 95: 1458-1462, 2010) (AU)

• Processo FAPESP: 06/00244-3 - Estudo da expressão do fator de crescimento IGF-II e dos fatores de transcrição SF-1 e DAX-1 em tumores adrenocorticais de crianças e adultos
• Beneficiário: Madson Queiroz Almeida
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 05/04726-0 - Caracterização molecular das doenças endócrinas congênitas que afetam o crescimento e o desenvolvimento
• Beneficiário: Ana Claudia Latronico Xavier
• Modalidade de apoio: Auxílio à Pesquisa - Temático