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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Steroidogenic Factor 1 Overexpression and Gene Amplification Are More Frequent in Adrenocortical Tumors from Children than from Adults

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Author(s):
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Almeida, Madson Q. [1] ; Soares, Ibere Cauduro [2] ; Ribeiro, Tamaya C. [1] ; Fragoso, Maria Candida B. V. [1] ; Marins, Lidiane V. [2] ; Wakamatsu, Alda [2] ; Ressio, Rodrigo A. [2] ; Nishi, Mirian Y. [1] ; Jorge, Alexander A. L. [1] ; Lerario, Antonio M. [1] ; Alves, Venancio A. F. [2] ; Mendonca, Berenice B. [1] ; Latronico, Ana Claudia [1]
Total Authors: 13
Affiliation:
[1] Univ Sao Paulo, Hosp Clin, Fac Med, Lab Hormonios & Genet Mol, Lab Invest Med, Unidade Endocrinol Desenvolvimento, Disciplina End, BR-05403900 Sao Paulo - Brazil
[2] Univ Sao Paulo, Hosp Clin, Fac Med, Div Anat Patol, Lab Patol Hepat LIM 14, BR-05403900 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM; v. 95, n. 3, p. 1458-1462, MAR 2010.
Web of Science Citations: 48
Abstract

Background: Steroidogenic factor 1 (SF-1) is a key determinant of endocrine development and function of adrenal cortex. SF-1 overexpression and gene amplification were previously demonstrated in a small group of pediatric adrenocortical tumors. Objective: Our objective was to determine the frequency of SF-1 protein expression and gene amplification in a large cohort of pediatric and adult adrenocortical tumors. Patients: SF-1 protein expression was assessed in a cohort of 103 adrenocortical tumors from 36 children and 67 adults, whereas gene amplification was studied in 38 adrenocortical tumors ( 17 from children). Methods: Tissue microarray, multiplex ligation-dependent probe amplification, and quantitative real-time PCR were used. Results: Astrong nuclear SF-1 expression was detected by tissue microarray in 56% (20 of 36) and 19% (13 of 67) of the pediatric and adult adrenocortical tumors, respectively (P = 0.0004). Increased SF-1 copy number was identified in 47% (eight of 17) and 10% (two of 21) of the pediatric and adult adrenocortical tumors, respectively (P = 0.02). All adrenocortical tumors with SF-1 gene amplification showed a strong SF-1 staining, whereas most of the tumors (61%) without SF-1 amplification displayed a weak or negative staining (P = 0.0008). Interestingly, a strong SF-1 staining was identified in five (29%) pediatric adrenocortical tumors without SF-1 amplification. The frequency of SF-1 overexpression and gene amplification was similar in adrenocortical adenomas and carcinomas. Conclusion: We demonstrated a higher frequency of SF-1 overexpression and gene amplification in pediatric than in adult adrenocortical tumors, suggesting an important role of SF-1 in pediatric adrenocortical tumorigenesis. (J Clin Endocrinol Metab 95: 1458-1462, 2010) (AU)

FAPESP's process: 06/00244-3 - Study of IGF-II growth factor expression and SF-1 and DAX-1 transcription factors in adrenocortical tumors in children and adults
Grantee:Madson Queiroz Almeida
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 05/04726-0 - Molecular characterization of congenital endocrine diseases that affect growth and development
Grantee:Ana Claudia Latronico Xavier
Support Opportunities: Research Projects - Thematic Grants