| Texto completo | |
| Autor(es): |
Gadelha, Ary
[1]
;
Machado, Mauricio F. M.
[2]
;
Yonamine, Camila M.
[3]
;
Sato, Joao R.
;
Juliano, Maria A.
[2]
;
Oliveira, Vitor
[2]
;
Bressan, Rodrigo A.
[1]
;
Hayashi, Mirian A. F.
[3]
Número total de Autores: 8
|
| Afiliação do(s) autor(es): | [1] Univ Fed Sao Paulo UNIFESP, Dept Psiquiatria, BR-04044020 Sao Paulo - Brazil
[2] Univ Fed Sao Paulo UNIFESP, Dept Biofis, BR-04044020 Sao Paulo - Brazil
[3] Univ Fed Sao Paulo UNIFESP, Dept Farmacol, BR-04044020 Sao Paulo - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF PSYCHIATRIC RESEARCH; v. 47, n. 5, p. 657-663, MAY 2013. |
| Citações Web of Science: | 16 |
| Resumo | |
Ndel1 oligopeptidase interacts with schizophrenia (SCZ) risk gene product DISCI and mediates several functions related to neurite outgrowth and neuronal migration. Ndel1 also hydrolyzes neuropeptides previously implicated in SCZ, namely neurotensin and bradykinin. Herein, we compared the plasma Ndel1 enzyme activity of 92 SCZ patients and 96 healthy controls (HCs). Ndel1 enzyme activity was determined by fluorimetric measurements of the FRET peptide substrate Abz-GFSPFRQ-EDDnp hydrolysis rate. A 31% lower mean value for Ndel1 activity was observed in SCZ patients compared to HCs (Student's t = 4.36; p < 0.001; Cohen's d = 0.64). The area under the curve (AUC) for the Receiver Operating Characteristic (ROC) curve for Ndel1 enzyme activity and SCZ/HCs status as outcome was 0.70. Treatment-resistant (TR) SCZ patients were shown to present a significantly lower Ndel1 activity compared to non-TR (NTR) patients by t-test analysis (t = 2.25; p = 0.027). A lower enzymatic activity was significantly associated with both NTR (p = 0.002; B = 1.19; OR = 3.29; CI 95% 1.57-6.88) and TR patients (p < 0.001; B = 2.27; OR = 9.64; CI 95% 4.12-22.54). No correlation between Ndel1 enzyme activity and antipsychotic dose, nicotine dependence, and body mass index was observed. This study is the first to show differences in Ndel1 activity in SCZ patients compared to HCs, besides with a significant lower activity for TR patients compared to NTR patients. Our findings support the Ndel1 enzyme activity implications to clinical practice in terms of diagnosis and drug treatment of SCZ. Objective of the study: To compare the Ndel1 enzyme activity levels of schizophrenia (SCZ) patients and healthy controls (HCs) and to correlate these values with the clinical profile and response to treatment by measuring the Ndel1 enzyme activity in human plasma. (C) 2013 Elsevier Ltd. All rights reserved. (AU) | |
| Processo FAPESP: | 03/00817-5 - Solicitação da patente da endooligopeptidase A |
| Beneficiário: | Mirian Akemi Furuie Hayashi |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa de Apoio à Propriedade Intelectual (PAPI/Nuplitec) |
| Processo FAPESP: | 08/57336-2 - Obtenção e caracterização das metacaspases: participação destas enzimas na morte celular |
| Beneficiário: | Mauricio Ferreira Marcondes Machado |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 09/17726-9 - Identificação e caracterização de ligantes da proteína Ndel1 (Nuclear distribution Element-Like), análise da regulação promotora e caracterização funcional da atividade enzimática |
| Beneficiário: | Mirian Akemi Furuie Hayashi |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 10/01394-4 - Métodos estatísticos e computacionais para a discriminação de alterações anatômicas, estados mentais e identificação da conectividade cerebral: uma abordagem integrativa utilizando ressonância magnética estrutural, funcional e EEG |
| Beneficiário: | João Ricardo Sato |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 11/50740-5 - Prevenção na esquizofrenia e no transtorno bipolar da neurociência à comunidade: uma plataforma multifásica, multimodal e translacional para investigação e intervenção |
| Beneficiário: | Rodrigo Affonseca Bressan |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |