| Full text | |
| Author(s): |
Gadelha, Ary
[1]
;
Machado, Mauricio F. M.
[2]
;
Yonamine, Camila M.
[3]
;
Sato, Joao R.
;
Juliano, Maria A.
[2]
;
Oliveira, Vitor
[2]
;
Bressan, Rodrigo A.
[1]
;
Hayashi, Mirian A. F.
[3]
Total Authors: 8
|
| Affiliation: | [1] Univ Fed Sao Paulo UNIFESP, Dept Psiquiatria, BR-04044020 Sao Paulo - Brazil
[2] Univ Fed Sao Paulo UNIFESP, Dept Biofis, BR-04044020 Sao Paulo - Brazil
[3] Univ Fed Sao Paulo UNIFESP, Dept Farmacol, BR-04044020 Sao Paulo - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | JOURNAL OF PSYCHIATRIC RESEARCH; v. 47, n. 5, p. 657-663, MAY 2013. |
| Web of Science Citations: | 16 |
| Abstract | |
Ndel1 oligopeptidase interacts with schizophrenia (SCZ) risk gene product DISCI and mediates several functions related to neurite outgrowth and neuronal migration. Ndel1 also hydrolyzes neuropeptides previously implicated in SCZ, namely neurotensin and bradykinin. Herein, we compared the plasma Ndel1 enzyme activity of 92 SCZ patients and 96 healthy controls (HCs). Ndel1 enzyme activity was determined by fluorimetric measurements of the FRET peptide substrate Abz-GFSPFRQ-EDDnp hydrolysis rate. A 31% lower mean value for Ndel1 activity was observed in SCZ patients compared to HCs (Student's t = 4.36; p < 0.001; Cohen's d = 0.64). The area under the curve (AUC) for the Receiver Operating Characteristic (ROC) curve for Ndel1 enzyme activity and SCZ/HCs status as outcome was 0.70. Treatment-resistant (TR) SCZ patients were shown to present a significantly lower Ndel1 activity compared to non-TR (NTR) patients by t-test analysis (t = 2.25; p = 0.027). A lower enzymatic activity was significantly associated with both NTR (p = 0.002; B = 1.19; OR = 3.29; CI 95% 1.57-6.88) and TR patients (p < 0.001; B = 2.27; OR = 9.64; CI 95% 4.12-22.54). No correlation between Ndel1 enzyme activity and antipsychotic dose, nicotine dependence, and body mass index was observed. This study is the first to show differences in Ndel1 activity in SCZ patients compared to HCs, besides with a significant lower activity for TR patients compared to NTR patients. Our findings support the Ndel1 enzyme activity implications to clinical practice in terms of diagnosis and drug treatment of SCZ. Objective of the study: To compare the Ndel1 enzyme activity levels of schizophrenia (SCZ) patients and healthy controls (HCs) and to correlate these values with the clinical profile and response to treatment by measuring the Ndel1 enzyme activity in human plasma. (C) 2013 Elsevier Ltd. All rights reserved. (AU) | |
| FAPESP's process: | 09/17726-9 - Identification and characterization of Ndel1 ligants, promoter regulation and characterization of the enzymatic activity |
| Grantee: | Mirian Akemi Furuie Hayashi |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 10/01394-4 - Statistical and computational methods for discriminating of anatomic changes, mental states and identification of brain connectivity: an integrative approach based on MRI, fMRI and EEG |
| Grantee: | João Ricardo Sato |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 11/50740-5 - Prevention in schizophrenia and bipolar disorder from neuroscience to the community: a multiphase, multimodal and translational platform for research and intervention |
| Grantee: | Rodrigo Affonseca Bressan |
| Support Opportunities: | Research Projects - Thematic Grants |