Biomass-to-bio-products application of feruloyl esterase from Aspergillus clavatus

Autor(es):Mostrar menos -

Damasio, Andre R. L. ^[1] ; Pinto Braga, Cleiton Marcio ^[1] ; Brenelli, Livia B. ^[1] ; Citadini, Ana Paula ^[1] ; Mandelli, Fernanda ^{[1, 2]} ; Cota, Junio ^{[1, 2]} ; de Almeida, Rodrigo Ferreira ^[1] ; Salvador, Victor Hugo ^[3] ; Alvaredo Paixao, Douglas Antonio ^[1] ; Segato, Fernando ^[1] ; Mercadante, Adriana Zerlotti ^[2] ; de Oliveira Neto, Mario ^[4] ; do Santos, Wanderley Dantas ^[3] ; Squina, Fabio M. ^[1]

Afiliação do(s) autor(es)

^[1] CNPEM, Lab Nacl Ciencia & Tecnol Bioetanol CTBE, BR-13083970 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Fac Engn Alimentos, Dept Ciencia Alimentos, Sao Paulo - Brazil

^[3] Univ Estadual Maringa, Dept Bioquim, Ctr Ciencias Biol, Maringa, Parana - Brazil

^[4] Univ Estadual Paulista, Inst Biociencias, Dept Fis & Biofis, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Applied Microbiology and Biotechnology; v. 97, n. 15, p. 6759-6767, AUG 2013.
• Citações Web of Science: 33

Resumo

The structural polysaccharides contained in plant cell walls have been pointed to as a promising renewable alternative to petroleum and natural gas. Ferulic acid is a ubiquitous component of plant polysaccharides, which is found in either monomeric or dimeric forms and is covalently linked to arabinosyl residues. Ferulic acid has several commercial applications in food and pharmaceutical industries. The study herein introduces a novel feruloyl esterase from Aspergillus clavatus (AcFAE). Along with a comprehensive functional and biophysical characterization, the low-resolution structure of this enzyme was also determined by small-angle X-ray scattering. In addition, we described the production of phenolic compounds with antioxidant capacity from wheat arabinoxylan and sugarcane bagasse using AcFAE. The ability to specifically cleave ester linkages in hemicellulose is useful in several biotechnological applications, including improved accessibility to lignocellulosic enzymes for biofuel production. (AU)

• Processo FAPESP: 11/02169-7 - Aspergillus nidulans como modelo para expressão heteróloga de celulases e hemicelulases
• Beneficiário: André Ricardo de Lima Damasio
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 09/17956-4 - Expressão e caracterização de feruloil esterases de Aspergillus clavatus
• Beneficiário: Cleiton Márcio Pinto Braga
• Linha de fomento: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 08/58037-9 - Geração de biblioteca para conversão enzimática de biomassa a partir de metagenoma do solo
• Beneficiário: Fábio Márcio Squina
• Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Apoio a Jovens Pesquisadores