Biomass-to-bio-products application of feruloyl esterase from Aspergillus clavatus

Author(s):Show less -

Damasio, Andre R. L. ^[1] ; Pinto Braga, Cleiton Marcio ^[1] ; Brenelli, Livia B. ^[1] ; Citadini, Ana Paula ^[1] ; Mandelli, Fernanda ^{[1, 2]} ; Cota, Junio ^{[1, 2]} ; de Almeida, Rodrigo Ferreira ^[1] ; Salvador, Victor Hugo ^[3] ; Alvaredo Paixao, Douglas Antonio ^[1] ; Segato, Fernando ^[1] ; Mercadante, Adriana Zerlotti ^[2] ; de Oliveira Neto, Mario ^[4] ; do Santos, Wanderley Dantas ^[3] ; Squina, Fabio M. ^[1]

Affiliation

^[1] CNPEM, Lab Nacl Ciencia & Tecnol Bioetanol CTBE, BR-13083970 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Fac Engn Alimentos, Dept Ciencia Alimentos, Sao Paulo - Brazil

^[3] Univ Estadual Maringa, Dept Bioquim, Ctr Ciencias Biol, Maringa, Parana - Brazil

^[4] Univ Estadual Paulista, Inst Biociencias, Dept Fis & Biofis, Sao Paulo - Brazil

• Document type: Journal article
• Source: Applied Microbiology and Biotechnology; v. 97, n. 15, p. 6759-6767, AUG 2013.
• Web of Science Citations: 33

Abstract

The structural polysaccharides contained in plant cell walls have been pointed to as a promising renewable alternative to petroleum and natural gas. Ferulic acid is a ubiquitous component of plant polysaccharides, which is found in either monomeric or dimeric forms and is covalently linked to arabinosyl residues. Ferulic acid has several commercial applications in food and pharmaceutical industries. The study herein introduces a novel feruloyl esterase from Aspergillus clavatus (AcFAE). Along with a comprehensive functional and biophysical characterization, the low-resolution structure of this enzyme was also determined by small-angle X-ray scattering. In addition, we described the production of phenolic compounds with antioxidant capacity from wheat arabinoxylan and sugarcane bagasse using AcFAE. The ability to specifically cleave ester linkages in hemicellulose is useful in several biotechnological applications, including improved accessibility to lignocellulosic enzymes for biofuel production. (AU)

• FAPESP's process: 11/02169-7 - Aspergillus nidulans as a model for heterologous expression of cellulases and hemicellulases
• Grantee: André Ricardo de Lima Damasio
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 09/17956-4 - Expression and characterization of feruloyl esterases from Aspergillus clavatus
• Grantee: Cleiton Márcio Pinto Braga
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 08/58037-9 - Library generation for biomass-conversion enzymes from soil metagenome
• Grantee: Fábio Márcio Squina
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Young Investigators Grants