Detection of immunoglobulin G in the lung and liver of hamsters with visceral leishmaniasis

Autor(es)

R. Mathias ^[1] ; F.A.L. Costa ; H. Goto

Afiliação do(s) autor(es)

^[1] Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo. Laboratório de Soroepidemiologia e Imunobiologia Celular e Molecular

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Medical and Biological Research; v. 34, n. 4, p. 539-543, 2001-04-00.

Resumo

Several organs are affected in visceral leishmaniasis, not only those rich in mononuclear phagocytes. Hypergammaglobulinemia occurs during visceral leishmaniasis; anti-Leishmania antibodies are not primarily important for protection but might be involved in the pathogenesis of tissue lesions. The glomerulonephritis occurring in visceral leishmaniasis has been attributed to immune complex deposition but in other organs the mechanism has not been studied. In the current study we demonstrated the presence of IgG in the lung and liver of hamsters with visceral leishmaniasis. Hamsters were injected intraperitoneally with 2 x 10(7) amastigotes of Leishmania (Leishmania) chagasi and the presence of IgG in the liver and lung was evaluated at 7, 15, 30, 45, 80 and 102 days postinfection (PI) by immunohistochemistry. The parasite burden in the spleen and liver increased progressively during infection. We observed a deposit of IgG from day 7 PI that increased progressively until it reached highest intensity around 30 and 45 days PI, declining at later times. The IgG deposits outlined the sinusoids. In the lung a deposit of IgG was observed in the capillary walls that was moderate at day 7 PI, but the intensity increased remarkably at day 30 PI and declined at later times of infection. No significant C3 deposits were observed in the lung or in the liver. We conclude that IgG may participate in the pathogenesis of the inflammatory process of the lung and liver occurring in experimental visceral leishmaniasis and we discuss an alternative mechanism other than immune complex deposition. (AU)

• Processo FAPESP: 98/12092-5 - Internalizacao de imunoglobulinas por celulas endoteliais na patogenese do processo inflamatorio intersticial na leishmaniose visceral em hamster.
• Beneficiário: Hiro Goto
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 98/15414-3 - Internalização de imunoglobulinas por células endoteliais na patogênese do processo inflamatório intersticial na leishmaniose visceral em hamster
• Beneficiário: Regiane Mathias
• Modalidade de apoio: Bolsas no Brasil - Mestrado