Antigenotoxic Properties of Chlorophyll b Against Cisplatin-Induced DNA Damage and its Relationship with Distribution of Platinum and Magnesium In Vivo

Autor(es)

Serpeloni, Juliana Mara ^[1] ; Batista, Bruno Lemos ^[2] ; Friedmann Angeli, Jose Pedro ^[3] ; Mazzaron Barcelos, Gustavo Rafael ^[4] ; Pires Bianchi, Maria de Lourdes ^[5] ; Barbosa, Jr., Fernando ; Greggi Antunes, Lusania Maria ^[7]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo. Sch Pharmaceut Sci Ribeirao Preto

^[2] Univ Sao Paulo. Sch Pharmaceut Sci Ribeirao Preto

^[3] Univ Sao Paulo. Dept Chem

^[4] Univ Sao Paulo. Sch Pharmaceut Sci Ribeirao Preto

^[5] Univ Sao Paulo. Sch Pharmaceut Sci Ribeirao Preto

^[7] Univ Sao Paulo. Sch Pharmaceut Sci Ribeirao Preto

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES; v. 76, n. 6, p. 345-353, MAR 1 2013.
• Citações Web of Science: 0

Resumo

The chemotherapeutic agent cisplatin (cDDP) is widely used to treat a variety of solid and hematological tumors. However, cDDP exerts severe side effects, such as nephrotoxicity, neurotoxicity, and bone-marrow suppression. The use of some dietary compounds to protect organs that are not targets in association with chemotherapy has been encouraged. This study evaluated the protective effects of chlorophyll b (CLb) on DNA damage induced by cDDP by use of single-cell gel electrophoresis (SCGE) assays. Further, this investigation also determined platinum (Pt) and magnesium (Mg) bioaccumulation in mice tissues after treatment with CLb alone and/or in association of cDDP (simultaneous treatment) by inductively coupled plasmamass spectroscopy (ICP-MS). All parameters were studied in peripheral blood cells (PBC), kidneys, and liver of mice after administration of CLb (0.2 or 0.5 mg/kg of body weight {[}b.w.]), cDDP (6 mg/kg b.w.), and the combination CLb 0.2 plus cDDP or CLb 0.5 plus cDDP. Pt accumulation in liver and kidneys was higher than that found in PBC, while DNA damage was higher in kidneys and liver than in PBC. Further, treatment with CLb alone did not induce DNA damage. Evidence indicates that genotoxic effects produced by cDDP may not be related to Pt accumulation and distribution. In combined treatments, CLb decreased DNA damage in tissues, but the PT contents did not change and these treatments also showed that CLb may be an important source of Mg. Thus, our results indicate that consumption of CLb-rich foods may diminish the adverse health effects induced by cDDP exposure. (AU)

• Processo FAPESP: 05/59552-6 - Avaliação integrada da estabilidade e propriedades funcionais de pigmentos naturais de alimentos
• Beneficiário: Adriana Zerlotti Mercadante
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 08/06793-4 - Atividade antigenotóxica de compostos da dieta e sua influência na expressão de genes de resposta ao estresse oxidativo
• Beneficiário: Juliana Mara Serpeloni
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 10/05096-8 - Atividade antigenotóxica de compostos da dieta e sua influência na expressão de genes de resposta ao estresse oxidativo
• Beneficiário: Lusânia Maria Greggi Antunes
• Modalidade de apoio: Auxílio à Pesquisa - Regular