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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Antigenotoxic Properties of Chlorophyll b Against Cisplatin-Induced DNA Damage and its Relationship with Distribution of Platinum and Magnesium In Vivo

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Author(s):
Serpeloni, Juliana Mara [1] ; Batista, Bruno Lemos [2] ; Friedmann Angeli, Jose Pedro [3] ; Mazzaron Barcelos, Gustavo Rafael [4] ; Pires Bianchi, Maria de Lourdes [5] ; Barbosa, Jr., Fernando ; Greggi Antunes, Lusania Maria [7]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo. Sch Pharmaceut Sci Ribeirao Preto
[2] Univ Sao Paulo. Sch Pharmaceut Sci Ribeirao Preto
[3] Univ Sao Paulo. Dept Chem
[4] Univ Sao Paulo. Sch Pharmaceut Sci Ribeirao Preto
[5] Univ Sao Paulo. Sch Pharmaceut Sci Ribeirao Preto
[7] Univ Sao Paulo. Sch Pharmaceut Sci Ribeirao Preto
Total Affiliations: 7
Document type: Journal article
Source: JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES; v. 76, n. 6, p. 345-353, MAR 1 2013.
Web of Science Citations: 0
Abstract

The chemotherapeutic agent cisplatin (cDDP) is widely used to treat a variety of solid and hematological tumors. However, cDDP exerts severe side effects, such as nephrotoxicity, neurotoxicity, and bone-marrow suppression. The use of some dietary compounds to protect organs that are not targets in association with chemotherapy has been encouraged. This study evaluated the protective effects of chlorophyll b (CLb) on DNA damage induced by cDDP by use of single-cell gel electrophoresis (SCGE) assays. Further, this investigation also determined platinum (Pt) and magnesium (Mg) bioaccumulation in mice tissues after treatment with CLb alone and/or in association of cDDP (simultaneous treatment) by inductively coupled plasmamass spectroscopy (ICP-MS). All parameters were studied in peripheral blood cells (PBC), kidneys, and liver of mice after administration of CLb (0.2 or 0.5 mg/kg of body weight {[}b.w.]), cDDP (6 mg/kg b.w.), and the combination CLb 0.2 plus cDDP or CLb 0.5 plus cDDP. Pt accumulation in liver and kidneys was higher than that found in PBC, while DNA damage was higher in kidneys and liver than in PBC. Further, treatment with CLb alone did not induce DNA damage. Evidence indicates that genotoxic effects produced by cDDP may not be related to Pt accumulation and distribution. In combined treatments, CLb decreased DNA damage in tissues, but the PT contents did not change and these treatments also showed that CLb may be an important source of Mg. Thus, our results indicate that consumption of CLb-rich foods may diminish the adverse health effects induced by cDDP exposure. (AU)

FAPESP's process: 05/59552-6 - Integrated evaluation of the stability and functional properties of natural pigments of foods
Grantee:Adriana Zerlotti Mercadante
Support type: Research Projects - Thematic Grants
FAPESP's process: 08/06793-4 - Antigenotoxic activity of compounds in the diet and its effect in the expression of genes in response to oxidative stress
Grantee:Juliana Mara Serpeloni
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 10/05096-8 - Antigenotoxic activity of compounds in diet and its effects in the expression of genes in response to oxidative stress
Grantee:Lusânia Maria Greggi Antunes
Support type: Regular Research Grants