Evaluate the influence of CXCL8 and CCL20 chemokines individually or in combination with cytokines on the immune and migratory profile of human dendritic cells and lymphocytes.

• Grant number: 18/00094-9
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2018
• End date: January 31, 2021
• Field of knowledge: Biological Sciences - Immunology - Cellular Immunology
• Principal Investigator: Luciana Cavalheiro Marti
• Grantee: Luciana Cavalheiro Marti
• Host Institution: Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE). Sociedade Beneficente Israelita Brasileira Albert Einstein (SBIBAE). São Paulo , SP, Brazil
• Associated researchers: FERNANDA AGOSTINI ROCHA ; Luiz Vicente Rizzo ; Patricia Severino

Abstract

Tissue cells, either at rest or activated by some pathological condition, are continuously secreting chemokines as a manner of sending information on their location and physiological state to the immune system. The precise positioning of leucocytes inside an organ is crucial for physiological or pathological responses to take place. Secondary lymphoid organs secrete chemokines to enable the traffic of lymphocytes and dendritic cells, as well as to allow their encounter and the eventual beginning of the immune response. However, a relevant question is whether the level of expression and the specificity of chemokine receptors interfere with the ability to translate the chemokine/receptor binding into a biological response. This subject needs to be further explored. Another important question is whether the cytokines present in the tissue microenvironment interfere with the functionality of chemokine receptors. In fact, some cytokines may be able to regulate the function and/or expression of these receptors in the immune system cells. Chemokine receptor signaling usually occurs via G-protein; it depends on multiple factors to be efficient, among which the nature of the target cell, the presence of cytokines, the existence of conventional and atypical receptors, the expression of receptor-phosphorylating kinases and, most probably, a combination of these factors. In this work we will evaluate the effect of IL-15 and IL32 on the CXCL8 and CCL20 chemokine signaling pathways and their respective receptors. IL-15 and IL-32 seem to interfere positively in the secretion of chemokines and on the expression of their respective receptors, and yet display important roles on the effector function of lymphocytes and dendritic cells. CXCL8 and CCL20 are important players in immune cell migration, but their regulation is complex and not fully understood. Results from this work may elucidate important factors implicated in lymphocyte and dendritic cells migration, their effector function and the consequent initiation of the immune response. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PACHECO, MESSIAS OLIVEIRA; ROCHA, FERNANDA AGOSTINI; ALOIA, THIAGO PINHEIRO ARRAIS; MARTI, LUCIANA CAVALHEIRO. Evaluation of Atypical Chemokine Receptor Expression in T Cell Subsets. CELLS, v. 11, n. 24, p. 16-pg., 2022-12-01. (18/00094-9)

FERREIRA, BIANCA O.; GAMARRA, LIONEL F.; NUCCI, MARIANA P.; OLIVEIRA, FERNANDO A.; REGO, GABRIEL N. A.; MARTI, LUCIANA. LN-Derived Fibroblastic Reticular Cells and Their Impact on T Cell Response-A Systematic Review. CELLS, v. 10, n. 5, MAY 2021. (18/00094-9)