Evaluation of phenotypic and functional profile of myeloid and plasmocytoid dendritic cells and TCD4+ and TCD8+ cells in lichen planus

• Grant number: 11/20740-3
• Support type: Regular Research Grants
• Duration: February 01, 2012 - July 31, 2014
• Field of knowledge: Biological Sciences - Immunology
• Principal researcher: Maria Notomi Sato
• Grantee: Maria Notomi Sato
• Home Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Lichen planus (LP) is a chronic inflammatory mucocutaneous disease of unknown etiology. It is estimated that LP affects approximately 1% of world population and may involve skin, nails, scalp and mucous membranes separately or simultaneously. There are several immunological parameters related to the innate or adaptive immune response in the LP, which would be essential to better understanding of the immunopathogenesis of the disease. The proposal of this project is to evaluate in the Lichen planus the influence of the innate immune response by myeloid (mDC) and plasmacytoid dendritic cells (pDC) and their influence in the generation of pro-inflammatory cells, regulatory cells as well as the cytotoxic effector cells. To evaluate the mononuclear cells response to the agonists of TLR 2-9 and in the inflamassoma generation in the cytokines production (IFN-alpha, TNF-alpha, IL-1beta); analyze the effect of these signaling pathways in the mDC and pDC, as well as evaluate the frequency of DCs in blood and tissue lesions. Furthermore, to assess the impact of DCs differentiated from monocytes or purified pDC in the activation of allogeneic cells. The CD4+ T cells response will be evaluated by the polyfunctionality to secreting cytokines (IL-17, TNF-alpha, IFN-gamma, IL-21) and the regulatory CD4+CD25+CD45RA-CD127-Foxp3+ T cells. The cytotoxic response mediated by NK cells and CD8 + T lymphocytes, will be analyzed by the phenotypic and polyfunctional profile. Furthermore, the purified CD8+ T cells will be assessed for the expression of cytokines/chemokines and receptors by the method of PCR array. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DOMINGUES, R.; PIETROBON, A. J.; CARVALHO, G. C.; PEREIRA, N. Z.; PEREIRA, N. V.; SOTTO, M. N.; AOKI, V.; DUARTE, A. J. S.; SATO, M. N. Lichen planus: altered AIM2 and NLRP1 expression in skin lesions and defective activation in peripheral blood mononuclear cells. Clinical and Experimental Dermatology, v. 44, n. 4, p. E89-E95, JUN 2019. Web of Science Citations: 0.

DE CARVALHO, GABRIEL COSTA; ARAUJO HIRATA, FABIANA YASUMOTO; DOMINGUES, ROSANA; FIGUEIREDO, CRISTINA ADELAIDE; ZANIBONI, MARIANA COLOMBINI; PEREIRA, NAIURA VIEIRA; SOTTO, MIRIAN NACAGAMI; AOKI, VALERIA; DA SILVA DUARTE, ALBERTO JOSE; SATO, MARIA NOTOMI. Up-regulation of HMGB1 and TLR4 in skin lesions of lichen planus. ARCHIVES OF DERMATOLOGICAL RESEARCH, v. 310, n. 6, p. 523-528, AUG 2018. Web of Science Citations: 2.

DOMINGUES, ROSANA; DE CARVALHO, GABRIEL COSTA; AOKI, VALERIA; DA SILVA DUARTE, ALBERTO JOSE; SATO, MARIA NOTOMI. Activation of myeloid dendritic cells, effector cells and regulatory T cells in lichen planus. JOURNAL OF TRANSLATIONAL MEDICINE, v. 14, JUN 10 2016. Web of Science Citations: 5.

COSTA DE CARVALHO, GABRIEL; DOMINGUES, ROSANA; ALMEIDA DE SOUSA NOGUEIRA, MARCELLE; CALVIELLI CASTELO BRANCO, ANNA C.; GOMES MANFRERE, KELLY C.; VIEIRA PEREIRA, NAIURA; AOKI, VALERIA; NACAGAMI SOTTO, MIRIAN; DA SILVA DUARTE, ALBERTO J.; NOTOMI SATO, MARIA. Up-regulation of Proinflammatory Genes and Cytokines Induced by S100A8 in CD8(+) T Cells in Lichen Planus. ACTA DERMATO-VENEREOLOGICA, v. 96, n. 4, p. 485-489, 2016. Web of Science Citations: 5.

DE SOUSA NOGUEIRA, MARCELLE ALMEIDA; BIANCARDI GAVIOLI, CAMILA FATIMA; PEREIRA, NATALLI ZANETE; DE CARVALHO, GABRIEL COSTA; DOMINGUES, ROSANA; AOKI, VALERIA; SATO, MARIA NOTOMI. Human endogenous retrovirus expression is inversely related with the up-regulation of interferon-inducible genes in the skin of patients with lichen planus. ARCHIVES OF DERMATOLOGICAL RESEARCH, v. 307, n. 3, p. 259-264, APR 2015. Web of Science Citations: 9.