Nutritive value of feedstuffs, dietary protein requirement, and physiological responses of piapara Leporinus obtusidens

• Grant number: 18/06405-6
• Support Opportunities: Regular Research Grants
• Duration: August 01, 2018 - November 30, 2020
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Animal Nutrition and Feeding
• Principal Investigator: Leonardo Susumu Takahashi
• Grantee: Leonardo Susumu Takahashi
• Host Institution: Faculdade de Ciências Agrárias e Tecnológicas. Universidade Estadual Paulista (UNESP). Campus de Dracena. Dracena , SP, Brazil
• Associated researchers: Francisco Javier Alarcón
• Associated scholarship(s): 20/01914-0 - Determination of hepatic and intestinal metabolic enzyme activities in piapara (Megaleporinus obtusidens) and establishment of routine laboratory protocols, BP.TT; 18/21871-3 - Methodologies for the biochemical determination of the activities of intestinal digestive and hepatic metabolic enzymes in piaparas (Megaleporinus obtusidens), BP.TT

Abstract

Despite the productive potential and demand in piapara (Leporinus obtusidens) both for consumption and for sport fishing, studies about piapara nutrition are very scarce and the nutritional values of feedstuffs are unknown. In the first experiment, the digestibility coefficients of dry matter, protein and energy of feedstuffs used in commercial diets will be determined. High-protein ingredients will be evaluated: tilapia processing by-product meal, poultry by-product meal, feathers and blood meal, meat bone meal, soybean meal, corn gluten and cottonseed meal. Also, high-energy ingredients will be evaluated: sorghum, corn, corn germ, rice bran and wheat bran. For seven days, the fish will receive the test diets and then transferred to aquariums for faeces collection. The results will be submitted to ANOVA and Tukey´s test (5%). In the second experiment, the requirement of digestible protein for piapara juveniles and its effects on the metabolism and nitrogen excretion will be determined. 360 juveniles (15g) will be used, distributed in 24 tanks of 130 L (15 fish/tank). Six isoenergetic diets will be formulated to contain increasing levels of digestible protein (21, 24, 27, 30, 33, 36% PD). After 60 days of feeding, fish will be weighted to evaluate the growth performance and nutrient retention, as well as: concentration of metabolites in the blood, tissue energy reserve indicators, enzymatic activity and indicators of muscle tissue quality. Every three days, the concentration of total ammonia, nitrate and nitrite in the water of the tanks will be determined. The experimental design will be completely randomized, with 6 treatments and 4 replicates. The performance results will be submitted to ANOVA and polynomial regression. The results of physiological indicators and water parameters will be submitted to ANOVA and Tukey's test (5%). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TANAKA, RENATA AKEMI; SANTANA DE ALMEIDA, VIVIANE DO NASCIMENTO; TAVARES DE FARIAS, CAROLINA VASCONCELOS; SOUSA, LUANA CAMARGO; CARLI, GABRIELA CASTELLANI; MENEGASSO MANSANO, CLEBER FERNANDO; TAKAHASHI, LEONARDO SUSUMU. ENERGY AND PROTEIN INGREDIENTS FOR USE IN PIAPARA (Megaleporinus obtusidens) DIETS: NUTRITIVE VALUE AND INTESTINAL MORPHOMETRY. BOLETIM DO INSTITUTO DE PESCA, v. 47, 2021. (18/21871-3, 18/06405-6)