145/5000 Use of IPS cells and animal models to elucidate the pathophysiology of post-lingual sensorineural hearing loss of genetic etiology

• Grant number: 18/03433-9
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2018
• End date: August 31, 2021
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Karina Lezirovitz Mandelbaum
• Grantee: Karina Lezirovitz Mandelbaum
• Host Institution: Hospital das Clínicas da Faculdade de Medicina da USP (HCFMUSP). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
• Associated researchers: Ana Carla Batissoco

Abstract

Hearing loss is the most frequent sensory defect in humans, can have social, financial, vocational and educational impact for life. The study of deafness genes has contributed decisively to the knowledge of auditory physiology and to the development of strategies of how to intervene to restore it. However, there is still a substantial fraction of deafness genes to be identified. This research project aims to confirm the identity of the DFNA58 gene and uncover the pathophysiological mechanism underlying this form of post-lingual and progressive hearing loss, present in a Brazilian family and whose locus was mapped in 2009 by the proponent of this grant. The biological pathway, that this novel deafness gene belongs to, was never described as involved in hearing. Thus, this project aims to prove that this gene is indeed essential to the normal physiology of hearing and to elucidate the underlying defect that leads to hearing impairment, recapitulating the effects the altered expression of this gene in a cellular and animal model, through two strategies: 1) to obtain IPS cells (induced pluripotent stem cells) from of the samples of individuals with hearing loss carriers of the duplication and noncarriers (normal hearing or hearing impaired with different clinical presentation) from the family, to differentiate them in neurons and hair cells and to compare them for the metabolic differences. 2) to study the effect of its altered expression on the development of the otic capsule in zebrafish. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DO NASCIMENTO, LARISSA REIS; VIEIRA-SILVA, GLEICIELE ALICE; KITAJIMA, JOAO PAULO FUMIO WHITAKER; BATISSOCO, ANA CARLA; LEZIROVITZ, KARINA. New Insights into the Identity of the DFNA58 Gene. GENES, v. 13, n. 12, p. 28-pg., 2022-12-01. (13/08028-1, 18/03433-9, 14/13071-6)

LEZIROVITZ, KARINA; VIEIRA-SILVA, GLEICIELE A.; BATISSOCO, ANA C.; LEVY, DEBORA; KITAJIMA, JOAO P.; TROUILLET, ALIX; OUYANG, ELLEN; ZEBARJADI, NAVID; SAMPAIO-SILVA, JULIANA; PEDROSO-CAMPOS, VINICIUS; et al. A rare genomic duplication in 2p14 underlies autosomal dominant hearing loss DFNA58. Human Molecular Genetics, v. 29, n. 9, p. 1520-1536, MAY 1 2020. (13/08028-1, 14/13071-6, 18/03433-9)

BATISSOCO, ANA CARLA; PEDROSO-CAMPOS, VINICIUS; PARDONO, ELIETE; SAMPAIO-SILVA, JULIANA; SONODA, CINDY YUKIMI; VIEIRA-SILVA, GLEICIELE ALICE; DA SILVA DE OLIVEIRA LONGATI, ESTEFANY UCHOA; MARIANO, DIEGO; HOSHINO, ANA CRISTINA HIROMI; TSUJI, ROBINSON KOJI; et al. Molecular and genetic characterization of a large Brazilian cohort presenting hearing loss. Human Genetics, OCT 2021. (14/13071-6, 18/03433-9)

KOBAYASHI, GERSON S.; VIEIRA-SILVA, GLEICIELE A.; VARELLA-BRANCO, ELISA; MOREIRA, DANIELLE P.; KITAJIMA, JOAO PAULO F. W.; HEMZA, CLAUDIA R. M. L.; MINGRONI-NETTO, REGINA C.; LOJUDICE, FERNANDO H.; OITICICA, JEANNE; BENTO, RICARDO F.; et al. Generation of four induced pluripotent stem cells lines from PBMC of the DFNA58 family members: Two hearing-impaired duplication carriers (USPi006-A e USPi007-A) and two normal-hearing noncarriers (USPi004-A and USPi005-A). STEM CELL RESEARCH, v. 71, p. 5-pg., 2023-09-01. (13/08028-1, 18/03433-9, 14/13071-6)