NMR methods for plant cell wall characterization: development and applications to second generation biofuel technologies

• Grant number: 17/24465-3
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
• Start date: August 01, 2018
• End date: July 31, 2020
• Field of knowledge: Biological Sciences - Biophysics
• Principal Investigator: Eduardo Ribeiro de Azevedo
• Grantee: Eduardo Ribeiro de Azevedo
• Host Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• City of the host institution: São Carlos

Abstract

The production of advanced biofuels from lignocellulosic renewable feedstocks (such as sugarcane bagasse, for example) requires to hydrolyse the biomass polysaccharides into simple sugars. The efficiency of such a process depends mostly on the development of effective pretreatment processes and on less expensive and more efficient enzymatic cocktails. Thus, it is necessary to decrease the recalcitrance of the lignocellulosic materials, thus facilitating the enzymatic hydrolysis to fermentable sugars. However, to achieve that a detailed comprehension of the plant cell wall and its modification upon pre-treatments is highly desirable. Nuclear Magnetic Ressonance (ssNMR) provides many methods that can be used to achieve this goal, allowing to obtain information about chemical composition, structure and dynamics of the cell wall. Thus, in this proposal we intent to developed approach based on both solid-state Nuclear Magnetic Ressonance (ssNMR) and Time Domain Nuclear Magnetic Ressonance (TDNMR) to obtainan in-depth characterization of the plant cell walls in terms of their composition, architecture, morphology, crystallinity and other biophysical and biochemical properties relevant to biomass recalcitrance. Furthermore, we will carry out a number of specific studies aiming to understand in detail how plant cell wall composition, molecular, nano and micro structures are affected by a variety of pretreatments that are applied to biomass samples during its conversion into biofuels and chemicals, and how these modifications correlate with the enzymatic digestibility. (AU)

Scientific publications

(11)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GARCIA, RODRIGO H. S.; FILGUEIRAS, JEFFERSON G.; DEAZEVEDO, EDUARDO R.; COLNAGO, LUIZ ALBERTO. Power-optimized, time-reversal pulse sequence for a robust recovery of signals from rigid segments using time domain NMR. SOLID STATE NUCLEAR MAGNETIC RESONANCE, v. 104, DEC 2019. (17/02197-7, 17/24465-3)

MUNARO, ANA P.; DA CUNHA, GIOVANNI P.; FILGUEIRAS, JEFFERSON G.; PINTO, JOSE M.; MUNARO, MARILDA; DE AZEVEDO, EDUARDO R.; AKCELRUD, LENI C.. Ageing and structural changes in PDMS rubber investigated by time domain NMR. Polymer Degradation and Stability, v. 166, p. 300-306, AUG 2019. (17/24465-3)

GARCIA, RODRIGO HENRIQUE DOS SANTOS; FILGUEIRAS, JEFFERSON GONCALVES; COLNAGO, LUIZ ALBERTO; DE AZEVEDO, EDUARDO RIBEIRO. eal-Time Monitoring Polymerization Reactions Using Dipolar Echoes in H-1 Time Domain NMR at a Low Magnetic Fiel. Molecules, v. 27, n. 2, JAN 2022. (19/13656-8, 17/24465-3, 21/12694-3)

NOVOTNY, ETELVINO H.; GARCIA, RODRIGO H. S.; DEAZEVEDO, EDUARDO R.. Pulse sequence induced variability combined with multivariate analysis as a potential tool for 13C solid-state NMR signals separation, quantification, and classification. JOURNAL OF MAGNETIC RESONANCE OPEN, v. 14-15, p. 9-pg., 2023-06-01. (09/18354-8, 19/12885-3, 17/24465-3)

FACCHINATTO, WILLIAM MARCONDES; DOS SANTOS, DANILO MARTINS; FIAMINGO, ANDERSON; BERNARDES-FILHO, RUBENS; CAMPANA-FILHO, SERGIO PAULO; DE AZEVEDO, EDUARDO RIBEIRO; COLNAGO, LUIZ ALBERTO. Evaluation of chitosan crystallinity: A high-resolution solid-state NMR spectroscopy approach. Carbohydrate Polymers, v. 250, DEC 15 2020. (17/24465-3, 16/20970-2, 17/20973-4, 16/09720-4, 19/13656-8)

NOVOTNY, ETELVINO HENRIQUE; DEAZEVEDO, EDUARDO RIBEIRO; DE GODOY, GUSTAVO; CONSALTER, DANIEL MARTELOZO; COOPER, MIGUEL. Determination of soil pore size distribution and water retention curve by internal magnetic field modulation at low field 1H NMR. Geoderma, v. 431, p. 15-pg., 2023-02-09. (09/18354-8, 17/24465-3)

PEREZ, MARTHA GONZALES; LIMA, ALINE PINDE; MORAES, TIAGO BUENO; CHAVES, ERICA GERVASONI; DA SILVA RUIZ, NAIRA MACHADO; DOS SANTOS TEIXEIRA, SYLVIA CORREA; HONORATO, HERCILIO DE ANGELI; CABRAL DE MENEZES, SONIA MARIA; DEAZEVEDO, EDUARDO RIBEIRO. H-1 Time Domain NMR to probe microstructural and mobility changes in Polyamide 11 exposed to H2S scavengers. What type of information can be assessed?. Polymer Degradation and Stability, v. 202, p. 10-pg., 2022-06-05. (17/24465-3, 09/18354-8)

COTA ELIAS, MEGG MADONYK; DIAS FERREIRA, GABRIEL MAX; REZENDE DE ALMEIDA, FRANCINE TATIANE; MARTINS ROSA, NATHALIA CRISTINA; SILVA, ISABELA ALMEIDA; FILGUEIRAS, JEFFERSON GONCALVES; DE AZEVEDO, EDUARDO RIBEIRO; MENDES DA SILVA, LUIS HENRIQUE; SACRAMENTO MELO, TANIA MARCIA; GIL, LAURENT FREDERIC; et al. Synthesis and application of sugarcane bagasse cellulose mixed esters. Part I: Removal of Co2+ and Ni2+ from single spiked aqueous solutions in batch mode using sugarcane bagasse cellulose succinate phthalate. Journal of Colloid and Interface Science, v. 533, p. 678-691, JAN 1 2019. (17/24465-3)

BESSER, KATRIN; MALYON, GRAHAM P.; EBORALL, WILLIAM S.; DA CUNHA, GIOVANNI PARO; FILGUEIRAS, JEFFERSON G.; DOWLE, ADAM; GARCIA, LOURDES CRUZ; PAGE, SAMUEL J.; DUPREE, RAY; KERN, MARCELO; et al. Hemocyanin facilitates lignocellulose digestion by wood-boring marine crustaceans. NATURE COMMUNICATIONS, v. 9, DEC 3 2018. (15/13684-0, 17/24465-3)

REZENDE DE ALMEIDA, FRANCINE TATIANE; COTA ELIAS, MEGG MADONYK; PEDROSA XAVIER, AMALIA LUISA; DIAS FERREIRA, GABRIEL MAX; SILVA, ISABELA ALMEIDA; FILGUEIRAS, JEFFERSON GONCALVES; DE AZEVEDO, EDUARDO RIBEIRO; MENDES DA SILVA, LUIS HENRIQUE; GIL, LAURENT FREDERIC; ALVES GURGEL, LEANDRO VINICIUS. Synthesis and application of sugarcane bagasse cellulose mixed esters. Part II: Removal of Co2+ and Ni2+ from single spiked aqueous solutions in batch and continuous mode. Journal of Colloid and Interface Science, v. 552, p. 337-350, SEP 15 2018. (17/24465-3)

FERNANDES, HENRIQUE; FILGUEIRAS, JEFFERSON G.; DE AZEVEDO, EDUARDO R.; LIMA-NETO, BENEDITO S.. Real time monitoring by time-domain NMR of ring opening metathesis copolymerization of norbornene-based red palm olein monomer with norbornene. EUROPEAN POLYMER JOURNAL, v. 140, NOV 5 2020. (17/24465-3, 17/06329-5)