Advanced search
Start date
Betweenand

Photobiomodulation with light-emitting diode (led) can improve the efficiency of mesenquimial stem cell therapy in post-ischemic heart failure?

Grant number: 18/06865-7
Support type:Regular Research Grants
Duration: October 01, 2018 - September 30, 2020
Field of knowledge:Biological Sciences - Physiology
Principal Investigator:Andrey Jorge Serra
Grantee:Andrey Jorge Serra
Home Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
Assoc. researchers: Jose Renato Dias Oliveira Pinto ; Paulo de Tarso Camillo de Carvalho ; Paulo Jose Ferreira Tucci

Abstract

Myocardial infarction causes irreversible loss of cardiomyocytes, culminating in negative cardiac remodeling and heart failure (HF). Considering the limited intrinsic repair ability of myocardial, and a high morbidity and mortality due to HF even with the optimized therapeutic arsenal, searches for more effective alternatives to attenuate the cardiac remodeling post-infarction reigns in the literature. In this regard, mesenchymal stem cells (MSCs) transplantation is a promising approach to restore cardiac function. The MSCs expand easily ex vivo and show differentiation for endothelial cells and cardiomyocytes in vitro. Moreover, the paracrine benefits of MSCs arising from the secretion of growth factors and immunomodulatory (e.g., cytokines). Although experimental and clinical studies have illustrated benefits of MSCs in infarcted hearts, there are no singular findings. Main reasons for these findings are reduced cell adhesion in the organ and cell death linked to inhospitable microenvironment in the infarcted myocardium. Therefore, new approaches are needed to enhance cellular home and to improve the resistance as well as efficiency of CTMs. Using a preclinical myocardial infarction model in rats, we will investigate: 1) the hypothesis that light emitting diode (LED) stimulus can improve the viability and proliferation and, potentiate the secretion of cytokines and growth factors of mesenchymal stem cells of adipose tissue (CTMA). Additionally, we will test different irradiation parameters, targeting to determine the LED dose with more evident biological effects; 2) whether in vitro pre-irradiation with LED improves the therapeutic CTMA action in the infarcted heart. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
GRANDINETTI, VANESSA; CARLOS, FERNANDO P. EREIRA; ANTONIO, EDNEI LUIZ; DE OLIVEIRA, HELENITA ANTONIA; NEVES DOS SANTOS, LUIS FELIPE; YOSHIZAKI, AMANDA; DIAS MARTINS MANSANO, BARBARA SAMPAIO; SILVA, FLAVIO ANDRE; PORTE, LESLIE ANDREWS; ALBUQUERQUE-PONTES, GIANNA MOES; CAMILLO DE CARVALHO, PAULO DE TARSO; MANCHINI, MARTHA TRINDADE; LEAL-JUNIOR, ERNESTO CESAR; FERREIRA TUCCI, PAULO JOSE; SERRA, ANDREY JORGE. Photobiomodulation therapy combined with carvedilol attenuates post-infarction heart failure by suppressing excessive inflammation and oxidative stress in rats. SCIENTIFIC REPORTS, v. 9, JUL 1 2019. Web of Science Citations: 0.
DE LIMA, RAFAEL DO NASCIMENTO; VIEIRA, STELLA SOUSA; ANTONIO, EDNEI LUIZ; CAMILLO DE CARVALHO, PAULO DE TARSO; VIEIRA, RODOLFO DE PAULA; DIAS MARTINS MANSANO, BARBARA SAMPAIO; DE ARRUDA JUNIOR, DANIEL FERREIRA; COSTA GIRARDI, ADRIANA CASTELLO; FERREIRA TUCCI, PAULO JOSE; SERRA, ANDREY JORGE. Low-level laser therapy alleviates the deleterious effect of doxorubicin on rat adipose tissue-derived mesenchymal stem cells. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 196, JUL 2019. Web of Science Citations: 0.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.