Phylogeography, population genomics and speciation of naturally fragmented populations of Bromeliaceae

• Grant number: 18/07596-0
• Support Opportunities: BIOTA-FAPESP Program - Regular Research Grants
• Start date: November 01, 2018
• End date: April 30, 2021
• Field of knowledge: Biological Sciences - Genetics
• Principal Investigator: Clarisse Palma da Silva
• Grantee: Clarisse Palma da Silva
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated researchers: Cleber Juliano Neves Chaves ; Fábio Pinheiro

Abstract

Neotropics encompass large number of hotspot regions to conservation, however the knowledge about patters and process responsible to shape its outstanding biodiversity are still emerging. Multidisciplinary approaches such as, systematics, phylogeography, population genetics and genomics, ecology and morphometry can significantly contribute to identify important evolutionary processes in groups of species with difficult delimitation. This integrative approach can be useful in regions with high biodiversity, such as Neotropics, aiming sustainable use and conservation. Two critical issues are particularly relevant to species delimitation using integrative approaches. One is that of dealing with naturally fragmented populations. The other is making decisions about lineages that are currently hybridizing and introgressing. Those are challenges in characterizing biodiversity, especially in groups of recent radiation, such as bromeliads, with exceptional levels of beta-diversity and microendemism, especially in naturally fragmented habitats as the outcrops in Southeastern Brazil. In this project we aim to: 1) characterize genetic and morphological variation in a bromeliad species complex (Pitcairnia flammea), occurring in naturally fragmented habitat in the Atlantic rainforest; 2) contribute to species delimitation of this species complex using integrative analyses of phylogeography, population genomics, morphometry, and ecological niche modelling; 3) investigate reproductive isolation among population and lineages and the role of intra and inter-specific gene flow in maintain species integrity and cohesion among these lineages; 4) study the evolutionary history to infer mechanisms and processes responsible for phylogeographical patterns of this species complex by using (a) nuclear and plastidial sanger sequencing, and (b) a large set of SNPs isolated by RAD-seq; 5) combine molecular, genomic and NEM data sets to better understand how demographic history of these populations influenced current geographic distribution of genetic variation; 6) identify SNPs associated with candidate genes sob-selection in P. flammea populations, that may be responsible for local adaptation. Comparing neutral and adaptive genomic patterns will allow us to discuss the long term effects of population fragmentation in reproductive fitness and evolutionary potential of naturally fragmented populations. Furthermore, a better understanding of these processes will allow to preview the consequences of global climatic changes and the effects of naturally and anthropic fragmentation in speciation and adaptation in regions of outstanding biodiversity. These information are fundamental to understand processes responsible for shaping species diversity in these biomes, aiming sustainable use and conservations of these and other species adapted to fragmented habitats. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MOTA, MATEUS RIBEIRO; PINHEIRO, FABIO; DOS SANTOS LEAL, BARBARA SIMOES; SARDELLI, CARLA HAISLER; WENDT, TANIA; PALMA-SILVA, CLARISSE. From micro- to macroevolution: insights from a Neotropical bromeliad with high population genetic structure adapted to rock outcrops. HEREDITY, JUL 2020. (14/15588-6, 14/02377-7, 09/52725-3, 18/07596-0, 15/07685-4)

CACOSSI, TAMI; DANTAS-QUEIROZ, MARCOS VINICIUS; PALMA-SILVA, CLARISSE. Transferability of nuclear microsatellites markers to Vriesea oligantha (Bromeliaceae), an endemic species from Espinhaco Range, Brazil. Rev. bras. Bot., v. 42, n. 4, p. 727-733, DEC 2019. (18/07596-0)

TAVARES, MARILIA MANUPPELLA; FERRO, MILENE; LEAL, BARBARA SIMOES SANTOS; PALMA-SILVA, CLARISSE. Speciation with gene flow between two Neotropical sympatric species (Pitcairnia spp.: Bromeliaceae). ECOLOGY AND EVOLUTION, v. 12, n. 5, p. 14-pg., 2022-05-01. (18/07596-0, 19/24823-2, 09/52725-3, 14/15588-6)

DE RUSSO GODOY, FERNANDA MARIA; PAGGI, GECELE MATOS; PALMA-SILVA, CLARISSE. Cross-amplification of nuclear microsatellite markers in Aechmea distichantha Lem. (Bromeliaceae). Rev. bras. Bot., v. 42, n. 2, p. 353-359, JUN 2019. (18/07596-0)

DANTAS-QUEIROZ, MARCOS VINICIUS; CACOSSI, TAMI DA COSTA; LEAL, BARBARA SIMOES SANTOS; CHAVES, CLEBER JULIANO NEVES; VASCONCELOS, THAIS N. C.; VERSIEUX, LEONARDO DE MELO; PALMA-SILVA, CLARISSE. Underlying microevolutionary processes parallel macroevolutionary patterns in ancient neotropical mountains. Journal of Biogeography, JUN 2021. (18/07596-0)

DANTAS-QUEIROZ, MARCOS VINICIUS; CACOSSI, TAMI DA COSTA; LEAL, BARBARA SIMOES SANTOS; CHAVES, CLEBER JULIANO NEVES; VASCONCELOS, THAIS N. C.; VERSIEUX, LEONARDO DE MELO; PALMA-SILVA, CLARISSE. Underlying microevolutionary processes parallel macroevolutionary patterns in ancient neotropical mountains. Journal of Biogeography, v. 48, n. 9, p. 2312-2327, SEP 2021. (18/07596-0)

MOTA, MATEUS RIBEIRO; PINHEIRO, FABIO; DOS SANTOS LEAL, BARBARA SIMOES; SARDELLI, CARLA HAISLER; WENDT, TANIA; PALMA-SILVA, CLARISSE. From micro- to macroevolution: insights from a Neotropical bromeliad with high population genetic structure adapted to rock outcrops. HEREDITY, v. 125, n. 5, p. 353-370, NOV 2020. (14/02377-7, 14/15588-6, 18/07596-0, 15/07685-4, 09/52725-3)