Bacillus subtilis on attenuation of heat stress and modulation of ethylene in bean

• Grant number: 17/16202-2
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2018
• End date: January 31, 2021
• Field of knowledge: Agronomical Sciences - Agronomy - Soil Science
• Principal Investigator: Fabio Fernando de Araujo
• Grantee: Fabio Fernando de Araujo
• Host Institution: Pró-Reitoria de Pesquisa e Pós-Graduação. Universidade do Oeste Paulista (UNOESTE). Presidente Prudente , SP, Brazil
• City of the host institution: Presidente Prudente
• Associated researchers: Alessandra Ferreira Ribas ; Tiago Benedito dos Santos
• Associated scholarship(s): 20/11892-3 - Bacillus subtilis in the attenuation of heat stress and modulation of ethylene in bean, BP.TT; 19/02935-3 - Bacillus subtilis at the attenuation of thermal stress and modulation of ethylene in bean, BP.TT

Abstract

The increasing concentration of ethylene in plant has been related to the damage caused in the abscission of flowers and pods, as well as reduced nodulation on bean, occurring in periods of high air temperatures, this hormone is produced in plants initially with the formation of carboxylic-acid-aminociclopropano (ACC). On the other hand, it is known that the presence of rhizobacteria in plants can inhibit ethylene formation by deamination of ACC, carried out by the enzyme ACC-deaminase, in addition to trigger other biochemical changes in plants, when in stress conditions. Bacillus subtilis has great potential to mitigate the damage caused in plants by water, as: stresses salt and pathological. Proposed in this study to evaluate the effect of ethylene on flower abortion, nodulation and growth of bean, subjected to thermal stress, with or without inoculation of Bacillus subtilis, under controlled conditions. Will be studied also the transcriptional profile of genes involved in the biosynthesis of ethylene, such as: ACC synthase and oxidase. By biochemical and genomic analysis can evaluate if the inoculation of B. subtilis, using strains with high and low activity of ACC deaminase, verified in vitro, can influence the concentration of ethylene in plants and/or mitigate the damage of the thermal stress in bean. Finally, check the tolerance of different genotypes evaluated the thermal stress and the same behavior as ethylene concentrations in their tissues and damage to your development (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE LIMA, BRUNA COELHO; BONIFACIO, AURENIVIA; DE ALCANTARA NETO, FRANCISCO; ARAUJO, FABIO FERNANDO; FERREIRA ARAUJO, ADEMIR SERGIO. Bacillus subtilis rhizobacteria ameliorate heat stress in the common bean. RHIZOSPHERE, v. 21, p. 4-pg., 2022-01-11. (17/16202-2)

BETTIO, DANIELE PERRETI; ARAUJO, ADEMIR SERGIO FERREIRA; BONIFACIO, AURENIVIA; ARAUJO, FABIO FERNANDO DE. Soil microbial biomass, organic C, and soybean physiology in integrated crop-livestock systems with different inputs. ARCHIVES OF AGRONOMY AND SOIL SCIENCE, DEC 2020. (17/16202-2)

LIMA, B. C.; CRUZ, T. R.; RIBAS, A. F.; SANTOS, T. B.; CACEFO, V.; ARAUJO, F. F.. Gene expression and biochemical profiling in the mitigation of heat stress in common bean using Bacillus subtilis. Biologia Plantarum, v. 67, p. 11-pg., 2023-01-01. (17/16202-2, 21/10823-0)

BETTIO, DANIELE PERRETI; ARAUJO, ADEMIR SERGIO FERREIRA; BONIFACIO, AURENIVIA; DE ARAUJO, FABIO FERNANDO. Soil microbial biomass, organic C, and soybean physiology in integrated crop-livestock systems with different inputs. ARCHIVES OF AGRONOMY AND SOIL SCIENCE, v. 68, n. 7, p. 13-pg., 2020-12-25. (17/16202-2)

SALVADOR, GABRIELA LOZANO OLIVERIO; ARAUJO, FABIO FERNANDO; PEREIRA, ARTHUR PRUDENCIO DE ARAUJO; BONIFACIO, AURENIVIA; ARAUJO, ADEMIR SERGIO FERREIRA. Rhizobacteria and arbuscular mycorrhizal fungus presented distinct and specific effects on soybean growth when inoculated with organic compost. RHIZOSPHERE, v. 22, p. 5-pg., 2022-04-05. (17/16202-2)