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Evaluation of microRNAs in type 2 diabetic patients supplemented with açaí juice

Grant number: 17/09777-9
Support type:Regular Research Grants
Duration: February 01, 2019 - January 31, 2021
Field of knowledge:Health Sciences - Medicine - Medical Clinics
Principal Investigator:Elisa Mieko Suemitsu Higa
Grantee:Elisa Mieko Suemitsu Higa
Home Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
Associated scholarship(s):19/03491-1 - Evaluation of polyphenols, anthocyanins and antioxidant capacity of açaí juice, BP.TT

Abstract

Diabetes mellitus (DM) is one of the major causes of death related to several genetic and environmental factors. DM is a chronic and inflammatory disease characterized by hyperglycemia, being classified in DM1 and DM2 (inadequate secretion of insulin and resistance to the action of this hormone, respectively), both can affect multiple organ functions, including the kidney, which results in diabetic nephropathy (DN). Açai is a fruit, originating in the Amazon, rich in nutrients such as lipids, proteins, fibers, vitamins, minerals and antioxidants. Recent study developed by our group has shown that açai reduced markers such as iNOS, NF-kB, TNF-± in cells cultured in high glucose medium, suggesting inflammatory modulation through Nrf2 antioxidant system, in this diabetes model. MicroRNAs (miRNAs) are small non-coding RNAs, essential to the gene regulation and protein expression, which can modulate the inflammatory response and thus contribute to the pathogenesis of DM. The aim of the project is to analyze the miRNAs expression profile in diabetic patients supplemented with açai juice. Patients with DM2 will be recruited from UNIFESP outpatient clinics. There will be collected data on diet, anthropometric parameters, body composition, blood samples for glycemia, insulin, total protein and fractions, peptide C, nitric oxide, LPS, lipid profile, urea and creatinine, cytokines (IL-6, TNF-± and TGF-²1), expression of plasma miRNAs (-17, -21, -26a, -29a, -30b, -126, -146a, -155, -199a e -1934), protein content of NF-kB p65, Nrf2, phospho-IKK, catalase in peripheral blood mononuclear cells (PBMC) and, microalbuminuria, pre and post intervention with açai juice supplemented for 4 consecutive weeks. Significance will be defined as p <0.05. (AU)