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Multi-User Equipment approved in grant 2017/17303-7: single tilt cryotransfer holder

Grant number: 18/26394-9
Support type:Multi-user Equipment Program
Duration: March 01, 2019 - February 28, 2026
Field of knowledge:Biological Sciences - Biochemistry - Chemistry of Macromolecules
Principal researcher:Shaker Chuck Farah
Grantee:Shaker Chuck Farah
Home Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated research grant:17/17303-7 - Structure and function of bacterial secretion systems, AP.TEM
As informações de acesso ao Equipamento Multiusuário são de responsabilidade do Pesquisador responsável
EMU web page:http://ca.iq.usp.br/novo/paginas_view.php?idPagina=51
Type of equipment:Caracterização de Materiais - Microscopia eletrônica/iônica - Criogênica
Manufacturer: JEOL
Model: GAT 626

Abstract

Once installed, these accessories will enable us to analyze CryoEM samples at the Institute of Chemistry, USP. This set of accessories will be installed in JEOL transmission electron microscope model JEM-2100 (with high-resolution pole piece EM-20720) which is currently located at the Analytical Center of the Institute of Chemistry of USP. A) The cryoholder will allow the transfer of up to four frozen EM grids (samples) under the microscope at temperatures below -170 C. B) The temperature controller provides precision control and temperature feedback for TEM support and includes an interface that provides support status reading during imaging or data collection. C) The pumping station produces a clean vacuum in the Torr range of 5x10-6 (usually in less than 2 minutes). (AU)

Articles published in Agência FAPESP Newsletter about the research grant:
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