The role of medial prefrontal cortex mineralocorticoid and glucocorticoid receptors in the process of extinction of aversive memory in rats submitted to acute stress.

• Grant number: 17/14473-9
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2019
• End date: April 30, 2021
• Field of knowledge: Biological Sciences - Pharmacology - Neuropsychopharmacology
• Principal Investigator: Leonardo Resstel Barbosa Moraes
• Grantee: Leonardo Resstel Barbosa Moraes
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• City of the host institution: Ribeirão Preto

Abstract

The aim of the present study is to investigate the role of acute stress and mineralocorticoid and glucocorticoid receptors present in the prefrontal cortex in the memory extinction learning. For this purpose, male Wistar rats will submitted to a surgery for the implant of a radiotelemetric probe into the abdominal aorta for monitoring autonomic responses, and after, cannulae guide will be stereotaxically implanted bilaterally in in the infralimbic (IL) or prelimbic (PL) regions of the ventromedial prefrontal cortex (vmPFC) of rodents for drug administration. After recover from surgeries, the animals will be exposure to a single and brief session of an uncontrollable stress (restraint stress for 1 hour). On the seventh day after de acute restraint stress session they will be trained in contextual fear conditioning protocol, followed by extinction session and extinction retention session, all sessions with a 24 hour interval. The mineralocorticoid receptor antagonist (RU 23318), or glucocorticoid receptor antagonist (RU 486) or the vehicle will be administered to the animals by microinjections into the IL or PL, 10 minutes before or immediately after the extinction session. Our hypothesis is that the mineralocorticoid and glucocorticoid receptors are important for the extinction of aversive memory, and that acute stress produces a deficit in the conditioned fear extinction learning through changes in the expression of receptors for glucocorticoids in vmPFC, and morphological modifications in this brain structure. (AU)

Scientific publications

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

ULIANA, DANIELA L.; ANTERO, LEANDRO S.; BORGES-ASSIS, ANNA B.; ROSA, JESSICA; VILA-VERDE, CARLA; LISBOA, SABRINA F.; RESSTEL, LEONARDO B. M.. Differential modulation of the contextual conditioned emotional response by CB1 and TRPV1 receptors in the ventromedial prefrontal cortex: Possible involvement of NMDA/nitric oxide-related mechanisms. JOURNAL OF PSYCHOPHARMACOLOGY, v. 34, n. 9, JUL 2020. (17/24304-0, 17/14473-9)

BERTACCHINI, GABRIELA L.; SONEGO, ANDREZA B.; WERWORN, LARISSA FERNANDA M.; SOLOVI, SARAH R.; RESSTEL, LEONARDO B. M.. The conditioned emotional response evoked by contextual fear is modulated by lateral hypothalamus synaptic activity without the involvement of GABAA receptor activation. PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY, v. 141, p. 12-pg., 2025-08-30. (13/08674-0, 17/14473-9, 17/24304-0)

BERTACCHINI, GABRIELA L.; SONEGO, ANDREZA B.; LISBOA, SABRINA S. F.; LAGATTA, DAVI C.; RESSTEL, LEONARDO B. M.. The expression of contextual fear conditioning involves the dorsal hippocampus TRPV1 receptor interacting with the NMDA/NO/cGMP signalling pathway. British Journal of Pharmacology, v. N/A, p. 14-pg., 2024-11-12. (17/24304-0, 13/08674-0, 17/14473-9)