Synergistic activities to boost enzymatic lignocellulose oxidation using light and LPMOs

• Grant number: 18/22300-0
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2019
• End date: February 28, 2022
• Field of knowledge: Biological Sciences - Biophysics - Molecular Biophysics
• Agreement: Fonds de la Recherche Scientifique (F.R.S.- FNRS)
• Mobility Program: SPRINT - Projetos de pesquisa - Mobilidade
• Principal Investigator: Igor Polikarpov
• Grantee: Igor Polikarpov
• Principal researcher abroad: David Cannella
• Institution abroad: Université Libre de Bruxelles (ULB), Belgium
• Host Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Associated researchers: Eduardo Ribeiro de Azevedo
• Associated research grant: 15/13684-0 - Structural and functional studies of enzymes that participate in complex carbohydrates synthesis and degradation, AP.TEM

Abstract

The main challenge of the 21st century society in terms of energy, materials and chemicals is to diminish the dependence from fossil resources transitioning towards a Bio-based economy. Recently an efficient light-driven enzymatic system capable of oxidizing cellulose, which is the major fraction of the photosynthetically fixed carbon on Earth, has been reported. The system discovered is based on the ubiquitous enzymes termed lytic polysaccharide monooxygenase (LPMO) that degrade plant cell wall carbohydrates, starch, and chitin found in all ecosystems and photosynthetic pigment chlorophyll, activated by light. One of the collateral effects of shining light on the reaction mixture is a generation of hydrogen peroxide, recently shown to be an efficient compound for accelerating LPMO activity. Within the scope of this proposal we plan to study the synergic effects in LPMO activities and cellulases to learn how they harness the light induced electron transfer and to evaluate the role of the parallel production of hydrogen peroxide to power redox enzymes for degradation of lignocellulosic substrates. We will also conduct molecular, biophysical and structural studies of poorly studied LPMOs. Furthermore, we plan to exploit the acquired knowledge for designing new degrading synergistic mixtures of cellulases and LPMOs aimed for plant biomass deplymerization. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SEPULCHRO, V, ANA GABRIELA; PELLEGRINI, VANESSA O. A.; DIAS, LUCAS D.; KADOWAKI, MARCO A. S.; CANNELLA, DAVID; POLIKARPOV, IGOR. Combining pieces: a thorough analysis of light activation boosting power and co-substrate preferences for the catalytic efficiency of lytic polysaccharide monooxygenase MtLPMO9A. BIOFUEL RESEARCH JOURNAL-BRJ, v. 8, n. 3, p. 1454-1464, SUM 2021. (18/22300-0, 15/13684-0, 19/13569-8)

VELASCO, JOSMAN; SEPULCHRO, ANA GABRIELA V.; HIGASI, PAULA M. R.; PELLEGRINI, VANESSA O. A.; CANNELLA, DAVID; DE OLIVEIRA, LEANDRO CRISTANTE; POLIKARPOV, IGOR; SEGATO, FERNANDO. Light Boosts the Activity of Novel LPMO from Aspergillus fumigatus Leading to Oxidative Cleavage of Cellulose and Hemicellulose. ACS SUSTAINABLE CHEMISTRY & ENGINEERING, v. 10, n. 50, p. 16-pg., 2022-12-08. (19/22284-7, 18/22300-0, 19/01165-0, 21/06679-1, 14/18714-2, 15/13684-0)

NETO, MARIO DE OLIVEIRA; FERNANDES, ADRIANO DE FREITAS; PIIADOV, VASSILI; CRAIEVICH, ALDO FELIX; DE ARAUJO, EVANDRO ARES; POLIKARPOV, IGOR. SAXSMoW 3.0: New advances in the determination of the molecular weight of proteins in dilute solutions from SAXS intensity data on a relative scale. Protein Science, v. 31, n. 1, SI, NOV 2021. (18/22300-0, 15/13684-0)