Evolution of floral traits related to pollinator attraction in Vanilla (Orchidaceae)

• Grant number: 18/07357-5
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2019
• End date: March 31, 2022
• Field of knowledge: Biological Sciences - Ecology
• Principal Investigator: Emerson Ricardo Pansarin
• Grantee: Emerson Ricardo Pansarin
• Host Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated scholarship(s): 21/01763-4 - Evolution of floral traits related to pollinator attraction in Vanilla (Orchidaceae), BP.TT

Abstract

The pollination systems displayed by orchid flowers have inspired biologists over the centuries. However, despite the economic importance of the genus Vanilla, little attention has been given to the pollination mechanisms and attraction of pollinators in these plants. However, previous studies reveal the most pollination strategies seem to be related to the attraction of male Euglossine bees. The understanding of the pollination processes in Vanilla is important, since the fruit production in this genus is based exclusively on hand pollinations. The data on anatomy, histochemical of floral glands and analyses of floral fragrances are scarce when compared to the diversity of this orchid genus. Furthermore, the floral characters and the fragrance compounds have not, to date, been analyzed in an evolutionary context. The project aims to test the hypothesis of convergent evolution in Vanilla, since the pollination by Euglossine bees has been recorded in at least two clades within this genus. Furthermore, the project aims to test the hypothesis of variation in the reward production, compotion of floral fragrances and pattern of floral reflectance, since others pollinator groups have been recorded as pollinators in Vanilla. To test these hypotheses, studies on secretory structures, the cells and tissues involved in the secretion, the floral reflectance patterns, as well as the volatile compounds of at least 30 species of Vanilla will be performed. Additionally, cpDNA and nrDNA regions will be analyzed in order to investigate the phylogeny of this plant group. The flower reflectance will also be studied with a spectrophotometer. The floral fragrances will be analyzed by GC-MS. The flowers of each species also will be investigated on basis on morpho-anatomical characters. Based on the obtained data, the patterns of evolution of floral traits related to the pollinators, pollination mechanisms and floral fragrances in this genus will be analyzed. (AU)

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PANSARIN, EMERSON RICARDO; DE BARROS, FABIO; KOCH, ANA KELLY. Nomenclatural notes on species of Cleistes (Orchidaceae: Vanilloideae) described by Jo & atilde;o Barbosa Rodrigues. PHYTOTAXA, v. 496, n. 2, p. 179-188, APR 12 2021. (07/07445-7, 18/07357-5)

PANSARIN, E. R.. nravelling the enigma of seed dispersal in Vanill. Plant Biology, v. 23, n. 6, SEP 2021. (18/07357-5)

PANSARIN, EMERSON RICARDO; MENEZES, EULER DA LUZ FERNANDES. A new remarkable Vanilla Mill. (Orchidaceae) species endemic to the Espinhaco Range, Brazil: its phylogenetic position and evolutionary relationships among Neotropical congeners. PHYTOKEYS, v. N/A, n. 227, p. 15-pg., 2023-06-08. (18/07357-5)

PANSARIN, EMERSON R.; SUETSUGU, KENJI. Mammal-mediated seed dispersal in Vanilla: Its rewards and clues to the evolution of fleshy fruits in orchids. ECOLOGY, v. 103, n. 7, p. 5-pg., 2022-05-18. (18/07357-5)

COELHO FERREIRA, ALESSANDRO WAGNER; FRANKEN, EVELYN PEREIRA; PANSARIN, EMERSON RICARDO. Confirmation of the presence of Vanilla hartii Rolfe (Orchidaceae, Vanilloideae) in Brazil. Check List, v. 16, n. 4, p. 6-pg., 2020-01-01. (18/07357-5)

PANSARIN, EMERSON R.; AUGUSTO, SAMANTHA G.; FERREIRA, ALESSANDRO W. C.. Resurrection of Liparis inundata (Orchidaceae). PHYTOTAXA, v. 441, n. 3, p. 294-300, MAY 5 2020. (18/07357-5)

PANSARIN, E. R.; FERREIRA, A. W. C.. Evolutionary disruption in the pollination system of Vanilla (Orchidaceae). Plant Biology, v. 24, n. 1, NOV 2021. (18/07357-5)

ZOTRE, GERHARD; ALMEDA, FRANK; ARIAS, SALVADOR; HAMMEL, BARRY; PANSARIN, EMERSON. Do secondary hemiepiphytes exist?. JOURNAL OF TROPICAL ECOLOGY, v. 37, n. 6, p. 5-pg., 2021-11-01. (18/07357-5)

PANSARIN, E. R.. Non-species-specific pollen transfer and double-reward production in euglossine-pollinated Vanilla. Plant Biology, v. N/A, p. 8-pg., 2023-04-26. (18/07357-5)

PANSARIN, EMERSON R.; PEDRO, SILVIA R. M.; DAVIES, KEVIN L.; STPICZYNSKA, MALGORZATA. Evidence of floral rewards in Brasiliorchis supports the convergent evolution of food-hairs in Maxillariinae. AMERICAN JOURNAL OF BOTANY, v. 109, n. 5, p. 15-pg., 2022-05-19. (18/07357-5)