Development of Brazilian clay/isoniazid compounds for use in isoniazid delivery system

• Grant number: 19/01231-2
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2019
• End date: May 31, 2022
• Field of knowledge: Engineering - Materials and Metallurgical Engineering - Nonmetallic Materials
• Principal Investigator: Francisco Rolando Valenzuela Diaz
• Grantee: Francisco Rolando Valenzuela Diaz
• Host Institution: Escola Politécnica (EP). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Christiano Gianesi Bastos Andrade ; Esperidiana Augusta Barretos de Moura ; Jorge Alberto Soares Tenório ; LEONARDO GONDIM DE ANDRADE E SILVA ; Lilian Gregory ; Maria das Graças da Silva Valenzuela ; Marvin Marco Chambi Peralta ; Mauri Sergio Alves Palma ; Wang Shu Hui

Abstract

The use of clays as controlled drug delivery systems is an important strategy to improve the therapy of various diseases. Isoniazid (INH) is the drug most used in the treatment of tuberculosis, a disease that persists in today's societies and whose treatment is toxic and aggressive. The intercalation of isoniazid to clay leads to the formation of the clay/INH compound, constituting a controlled release system that can be used more efficiently than the current treatment model. The objective of this project is to study Brazilian clays as controlled release systems for INH. From 11 national samples of montmorillonites (MMT), 01 kaolin (K), one synthetic clay (EST), besides 03 sodium clays (Na + MMT) which will be imported, clay/INH compounds will be obtained, characterized, and analyzed for their controlled release profiles. The Brazilian clays will be evaluated in the natural, purified and sodified form. Purification is necessary to achieve pharmaceutical properties. The process of obtaining clay/INH compounds will be developed with the aid of adsorption isotherms. The controlled release profiles of the compounds will be studied with by using spectrophotometry in the UV-visible region. The results will be evaluated considering the structure of the natural, purified and sodified clays, and how the INH intercalates to these structures. Characterization of clays and their compounds using X-ray diffractometry (XRD) plus the Rietveld technique and spectrophotometry in the infrared (IR) region with quantification will be widely used to aid in the understanding of clay properties and their respective influences in the process for obtaining the compounds and in the releasing INH from the clay/INH compounds. Physical and chemical properties will be evaluated by swelling capacity, cation exchange capacity (CTC), plastic (PV) and apparent (AV) viscosities, specific surface area, chemical composition via X-ray fluorescence (FRX) and others. Antimicrobial activities of the clay/INH compounds will be evaluated using the Minimal Inhibitory Concentration (MIC) method. The results will describe the performance of Brazilian clays in compounds that can be used as controlled release systems of isoniazid for the treatment of tuberculosis. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

T. C. CARVALHO; B. B, MICHEL; M. G. SILVA-VALENZUELA; E. HILDEBRANDO; R. F. NEVES; F. R. VALENZUELA-DIAZ. Simple and fast process using hydrochloric acid for producing synthetic zinc-rich stevensite. Cerâmica, v. 68, n. 387, p. 285-293, 2022-09-16. (19/01231-2)

CARVALHO, THAMYRES; NEVES, ROBERTO; HILDEBRANDO, EDEMARINO; DE PAIVA, LUCILENE BETEGA; VALENZUELA-DIAZ, FRANCISCO R.. Organophilic Synthetic Stevensite-Zn: Synthesis and Characterization, an Alternative Simple Method. MINERALS, v. 12, n. 12, p. 11-pg., 2022-12-01. (19/01231-2)

PINHEIRO, GILMAR; CARVALHO, THAMIRES; MICHEL, BIANCA; ARJONA, JESSICA; BOBADILHA, MARGARITA; SILVA-VALENZUELA, MARIA; COSTA, TATIANA; VALENZUELA-DIAZ, FRANCISCO; LI, J; ZHANG, M; et al. Characterization of a Brazilian Kaolin and Its Sorption Ability to Mineral Oils. CHARACTERIZATION OF MINERALS, METALS, AND MATERIALS 2020, v. N/A, p. 7-pg., 2020-01-01. (19/01231-2)