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BONE HEALING EVALUATION AROUND THE IMPLANTS WITH LASER BEAM SURFACES WITH AND WITHOUT HA DEPOSITION.CHARACTERIZATION TOPOGRAPHIC, CELLS CULTURE,BIOMECHANICAL AND HISTOMETRIC ANALYSIS IN RABBITS.

Abstract

The purpose of this study was to evaluate cell parameters, and the biological and mechanical behavior of the interface between bone and implant before the machined surfaces (MS), modified by blasting followed by commercially available (SES), laser modified (LS) and laser modified with HA coating biomimetic method without heat treatment (LHS). The topographic characterization of the surfaces will be carried out, followed by the culture of primary osteogenic cells to obtain the cellular parameters related to osteogenesis. After an in vitro study, 20 Albinus rabbits will receive 80 implants of 3.75x10mm in surgical beds, spaced in the medial portion of the right and left tibiae, two implants of each surface in each tibia. In the 10 animals at the 4-week euthanasia period will be performed intramuscular application of calcein and alizarin. In the 2 and 4 week periods the animals will be anesthetized, and the stability coefficient of the implant will be measured in vivo, followed by the measurements of the torque removal at the implant located more proximal to the tibiofemoral joint. In these implants the topographic characterization of the surface will be performed by MEV-EDX for the qualitative/quantitative analysis of the neoformed bone with mensuration of Ca, P and O. In the more distal localized implants the tibio-femoral joint will be realized microtomographic analysis followed by processed for inclusion in resin to obtain non-decalcified cuts. Confocal laser microscopy will be performed on 10 animals from the 4-week euthanasia period followed by histometric analysis. Following all the blades will be stained by Stevenel blue and Alizarin red. The bone interface contact and neoformed bone area will be measured. The obtained values at the biomechanical, microtomographic, confocal laser microscopy (histometric) and histometric (blue Stevenel and alizarin red) analyses will be taken to the statistical analysis.Keywords: Dental Implants; Would healing; Surface; Ytterbium laser, Osseointegration. (AU)