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Chemokines in leptospirosis: Characterization of its antimicrobial potential and interference in the gene expression of virulence factors in Leptospira sp.

Grant number: 17/20903-6
Support type:Regular Research Grants
Duration: July 01, 2019 - June 30, 2022
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal Investigator:Josefa Bezerra da Silva
Grantee:Josefa Bezerra da Silva
Home Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
Assoc. researchers:Enéas de Carvalho ; Paulo Lee Ho

Abstract

Leptospirosis is a zoonosis of high public health relevance that affects different organs and presents from mild clinical manifestations to failure of multiple organs. Its pathological agent is the spirochaete Leptospira that is transmitted directly through contact with animal reservoirs, which excrete the bacterium in the urine, or through contact with contaminated soil and water. This study will evaluate the antimicrobial activity of several chemokines towards L. interrogans through survival analysis in Petroff-Hausser chamber and in vitro growth. The chemokines with best inhibitory activity will be selected and better characterized. In addition, a treatment to analyze of the selected chemokines will be performed on cell lines (RAW 264-7 or HepaRG or MRC-5) infected with L. interrogans. Supernatant and cells will be collected after the treatment for protein determination by ELISA, evaluation of protein expression using commercial kits and / or (ELISA) and analysis of gene transcripts from molecular tests (qPCR) to analyze possible induction or inhibition of the bacteria gene. This study will contribute to the clarification of the role of chemokines in leptospirosis, evaluating the potential antimicrobial mediators and their contribution to the gene expression of virulence factors of Leptospira and progression of the disease. (AU)