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Identification and biological screening in the search for antimicrobial agents produced by actinobacteria against citrus canker

Grant number: 19/00922-1
Support type:Research Grants - Innovative Research in Small Business - PIPE
Duration: November 01, 2019 - July 31, 2020
Field of knowledge:Physical Sciences and Mathematics - Chemistry
Principal Investigator:Tânia Petta
Grantee:Tânia Petta
Company:Actinobac Agrosciences Pesquisa e Desenvolvimento Ltda
CNAE: Atividades de apoio à agricultura
City: Ribeirão Preto
Assoc. researchers:Franklin Behlau ; Luiz Alberto Beraldo de Moraes
Associated scholarship(s):19/23201-8 - Identification and biological screening in the search for antimicrobial agents produced by actinobacteria against Citrus Canker, BP.PIPE

Abstract

Citrus Cancer is a disease caused by Gram-negative bacteria Xanthomonas citri subsp. citri (Xcc) and is among the diseases that most affect the different species of citrus in various parts of the world, causing expressive commertial impact in the citrus sector. Citrus canker has no cure and the most effective way of disease control is the elimination of the symptomatic plant (eradication) in regions where there are few diseased plants. In places of high incidence, such as in the state of São Paulo, the management with various measures including copper and windbreak is adopted. However, the application of copper does not prevent the entrance of the bacterium into the orchard. This helps to reduce the amount of symptoms in the plants and the fall of fruits. Another alarming factor is that the excessive use of copper poses a risk of contamination of the environment at various levels, in addition to causing stress on the orange trees this way slowing its development. Historically, actinobacteria are known as the major antibiotic-producing microorganisms, accounting for approximately 70% of all antibiotics marketed in both the pharmaceutical and agribusiness industries. However, few studies have been described in the literature evaluating the potential of actinobacteria in the control of Xcc. In this sense, the present project aims at evaluating the potential of application of crude extracts produced by actinobacteria in inhibiting the growth of Xcc and consequently in the control of citrus canker. The evaluations will be carried out using in vitro diffusion disc growth inhibition assays and in vivo protective and curative action assays using greenhouse orange tree seedlings under controlled conditions. The intrinsic characteristic of actinobacteria as promising sources of antibiotics and the urgente need to seek alternative antimicrobial agents capable of controlling Xcc bacteria with lower environmental impact is the main source of inspiration for the development of this research project. The team of researchers has a lot of experience in the area of concentration of the proposal and are very optimistic about the success that should be obtained in the project. (AU)