Bioprinting of vascular grafts and the bioinks used

• Grant number: 19/05274-8
• Support Opportunities: Research Grants - Innovative Research in Small Business - PIPE
• Start date: March 01, 2020
• End date: October 31, 2022
• Field of knowledge: Interdisciplinary Subjects
• Agreement: FINEP - PIPE/PAPPE Grant
• Principal Investigator: Ana Luíza Garcia Millás Massaguer
• Grantee: Ana Luíza Garcia Millás Massaguer
• Company: Soluções em Biotecnologia 3D - Elaboração de Projetos Ltda
• CNAE: Fabricação de máquinas e equipamentos de uso geral não especificados anteriormente ; Comércio varejista de outros produtos novos não especificados anteriormente ; Pesquisa e desenvolvimento experimental em ciências físicas e naturais
• Pesquisadores principais: Pedro Xavier Rodriguez Massaguer
• Associated researchers: Marcelo Henrique Napimoga ; Sonja Ellen Lobo
• Associated research grant: 17/15309-8 - Bioprinter development: customization of a 3D printing technology for production of three-dimensional cellular structures, AP.PIPE
• Associated scholarship(s): 21/01637-9 - 3D bioprinting of vascular grafts and vascularized skin tissues, BP.PIPE; 21/02406-0 - Projects, modeling and construction related to 3D bio printers for fabrics and models in vitro, BP.TT; 20/14299-1 - Production of vascularized structures via combination of 3D bioprinting and electrospinning techniques: tubular structures and in vitro models, BP.TT; 20/13461-0 - Production of vascularized structures via combination of bioprinter and electrospinning technologies: tubular structures and in vitro models, BP.TT

Abstract

Tissue engineering is a multidisciplinary and interdisciplinary field that aims to create living biological substitutes with the purpose of partially or totally improve or replace tissues or organs that have been affected by some disease or injury. In addition, there is a part of the discipline that develops in vitro models as an alternative to the use of animals in safety assessment trials. Seeking to mimic reality on the nano, micro, and macro scales of tissues and organs, technologies such as 3D bioprinting and electrospinning can change the way many diseases are treated by replacing damaged tissues with newly created live bioconstructs. With 3D bioprinting it is possible to generate precise and complex multicellular constructions. In addition, it allows precise deposition of individual cells, growth factors and biochemical signals to better meet the specificities of a vascular graft, for example. As is known, vascular networks play an important role in the transport of nutrients, oxygen, metabolic residues and maintenance of homeostasis. The manufacture of vascularized and tubular constructions has remained a challenge so far, but is considered crucial as a starting point for bringing organ engineering to a higher level. With the advancement of bioprinting technology and the knowledge of biomaterials, it is expected that bioprinting can be a viable solution to this problem. Combining bioimpression technology with microextrusion of tubular structures validated during the PIPE Phase I, by using bioprinting and electrospinning techniques, this proposal aims to develop two vascular grafts, A) one acellular and B) one cell-ladden vascular graft, performing the stage of pre-clinical tests in animal model. In addition, validate the bioinks used to be available to the market. Regarding the cell culture stages, the proposal foresees the construction of our own Clean Room lab and for the pre clinical tests in an animal model, we have the collaboration of Prof. Dr. Nivaldo Alonso and PhD. Sonja Ellen Lobo of the Laboratory of Surgery of the Faculty of Medicine of USP. From a market perspective, it is worth mentioning that the few prototypes of bioprinters available in the market are manufactured abroad and that to date, 3DBS is the only national startup developing technology in this field and providing bioprinting products. Participation in PIPE Phase II project will allow us to continue the consolidation of competences aimed at biofabrication and also the consolidation of an own infrastructure adequate to the biosafety requirements to scale the production of biomaterials and biofabricates. This will allow us to enter the market for bio-manufacturing products, specifically therapeutic applications, offering vascular vessels capable of benefiting a number of patients with different vascular diseases, such as stenosis and occlusion of blood vessels. In addition, it will allow us to expand our portfolio of projects by offering specific and customized bioprinter services and courses and theoretical-practical training in the field of bioprinting. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

HENRIQUE ABDALLA; RAFAEL STORINO; AMANDA BANDEIRA; LUCAS TEIXEIRA; ANA MILLÁS; PAULO LISBOA-FILHO; KAMILA KANTOVITZ; FRANCISCO NOCITI JUNIOR. Glycogen synthase kinase 3 inhibition enhances mineral nodule formation by cementoblasts in vitro. Brazilian Oral Research, v. 37, 2023-11-10. (19/04276-7, 19/05274-8)

ABDALLA, HENRIQUE; STORINO, RAFAEL; BANDEIRA, AMANDA; TEIXEIRA, LUCAS; MILLAS, ANA; LISBOA-FILHO, PAULO; KANTOVITZ, KAMILA; NOCITI JUNIOR, FRANCISCO. Glycogen synthase kinase 3 inhibition enhances mineral nodule formation by cementoblasts in vitro. BRAZILIAN ORAL RESEARCH, v. 37, p. 10-pg., 2023-01-01. (19/04276-7, 19/05274-8)

ABDALLA, HENRIQUE B.; MARCHIORO, RAYSSA R.; GALVAO, KAREN ELIZABETH A.; TEIXEIRA, LUCAS N.; KANTOVITZ, KAMILA R.; MILLAS, ANA LUIZA G. M.; NOCITI JR, FRANCISCO H.. Polycaprolactone scaffolds as a biomaterial for cementoblast delivery: An in vitro study. JOURNAL OF PERIODONTAL RESEARCH, v. 57, n. 5, p. 10-pg., 2022-08-05. (19/04276-7, 19/05274-8)

CATORI, DANIELE M.; LOREVICE, MARCOS, V; DA SILVA, LAURA C. E.; CANDIDO, GEOVANY; MILLAS, ANA L. C.; DE OLIVEIRA, MARCELO C.. Fresh 3D Printing of Double Crosslinked Hyaluronic Acid/Pectin Hydrogels. MACROMOLECULAR SYMPOSIA, v. 406, n. 1, p. 8-pg., 2022-12-01. (19/05274-8, 18/14142-5, 21/00491-0, 16/02414-5)