3D pritting of bioactive and resorbable scaffolds by robocasting: synthesis, processing and antibacterial activity

• Grant number: 19/19594-4
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2020
• End date: October 31, 2022
• Field of knowledge: Engineering - Materials and Metallurgical Engineering - Nonmetallic Materials
• Principal Investigator: Eliandra de Sousa Trichês
• Grantee: Eliandra de Sousa Trichês
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Federal de São Paulo (UNIFESP). Campus São José dos Campos. São José dos Campos , SP, Brazil
• City of the host institution: São José dos Campos
• Associated researchers: Alexandre Luiz Souto Borges ; Antonio Pedro Novaes de Oliveira ; Dayane Batista Tada ; Elisângela Guzi de Moraes ; Marco Cicero Bottino ; Mariana Motisuke
• Associated scholarship(s): 21/02344-5 - Optimization of the rheological and injectability parameters of Beta-TCP/BG S53P4 ceramic pastes for processing via robocasting, BP.TT

Abstract

Osteomyelitis is a typical bone infection mainly caused by the bacteria Staphylococcus aureus, followed by other species. Strategies aiming the development of bioactive and resorbable scaffolds, carriers with growth factors, drugs and nanoparticles have become an alternative to the treatment of this pathology, and is the focus of bone tissue engineering. Among used biomaterials to scaffolds manufacturing aiming bone tissue recovery and regeneration, the calcium phosphate-based ceramics stand out due to their similarly, in terms of structure and chemical composition, to mineral phase of bone, and their excellent biocompatibility. ²-tricalcium phosphate (²-TCP) is considered resorbable and has excellent osteoconductivity. In an attempt to achieve good densification of the ²-TCP scaffold and improve its biological properties, it arises the possibility to combine this material with S53P4 bioactive glass, which composition is 53% SiO2, 23% Na2O, 20 % CaO and 4 % P2O5 (wt.%). This bioglass also presents antibacterial property and is already used in the osteomyelitis treatment, in the form of granules. Several techniques are employed to bioceramic scaffolds production and among them, the robocasting, a type of additive manufacture which allows the production of customized parts, highly complex and good reproducible. Moreover, the ²-TCP/S53P4 scaffold surface can be modified by coating with nanoparticles capable of releasing therapeutic ions (Ag+, Zn2+, Cu2+) aiming to potentialize the scaffold antibacterial effect and consequently decrease the patient's infection incidences. In this context, this project aims to produce and characterize ²-TCP / S53P4 scaffolds by robocasting, followed by coating its surface with nanoparticles capable of releasing therapeutic ions, aiming at the treatment of osteomyelitis. The scaffolds will be characterized by their morphological, physical, mechanical and biological properties, as well as their antibacterial activity in different cultures of microorganisms. (AU)

Scientific publications

(16)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NOGUEIRA ALVES, ANA PAULA; ARANGO-OSPINA, MARCELA; MORAES SALDANHA OLIVEIRA, RODRIGO LUIZ; FERREIRA, IGOR MAIA; DE MORAES, ELISANGELA GUZI; HARTMANN, MALTE; NOVAES DE OLIVEIRA, ANTONIO PEDRO; BOCCACCINI, ALDO ROBERTO; TRICHES, ELIANDRA DE SOUSA. 3D-printed beta-TCP/S53P4 bioactive glass scaffolds coated with tea tree oil: Coating optimization, in vitro bioactivity and antibacterial properties. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, v. 111, n. 4, p. 14-pg., 2022-11-28. (19/19187-0, 19/08141-9, 19/19594-4, 20/12944-7)

VERÔNICA RIBEIRO DOS SANTOS; SAMARA DOMINGUES VERA; GABRIELLE LUPETI DE CENA; ADRIELLE DE PAULA SILVA; ANA PAULA LEMES; KÁTIA DA CONCEIÇÃO; DAYANE BATISTA TADA; ALEXANDRE LUIZ SOUTO BORGES; ELIANDRA DE SOUSA TRICHÊS. Electrospun PHBV nanofiber containing Tea Tree Oil: physicochemical and antimicrobial activity. POLIMEROS-CIENCIA E TECNOLOGIA, v. 33, n. 1, 2023-04-14. (19/10877-3, 19/19594-4, 20/04455-6)

SPIRANDELI, B. R.; MARTINS, E. F.; DONA, L. R. M.; RIBAS, R. G.; CAMPOS, T. M. B.; ESPOSITO, E.; THIM, G. P.; TADA, D. B.; TRICHES, E. S.. Synergistic Effect of Incorporation of BG 45S5 and Silver Nanoparticles on beta-TCP Scaffolds: Structural Characterization and Evaluation of Antimicrobial Activity and Biocompatibility. MATERIALS RESEARCH-IBERO-AMERICAN JOURNAL OF MATERIALS, v. 26, p. 12-pg., 2023-01-01. (19/19594-4)

OLIVEIRA, RODRIGO LUIZ MORAES SALDANHA; ANTONELLI, EDUARDO; MONTUFAR, EDGAR BENJAMIN; TRICHES, ELIANDRA DE SOUSA. Cold sintering of β-tricalcium phosphate/bioactive glass composites. CERAMICS INTERNATIONAL, v. 50, n. 11, p. 8-pg., 2024-04-20. (19/19594-4, 21/05259-9)

DOS SANTOS, VERONICA RIBEIRO; CAMPOS, TIAGO MOREIRA BASTOS; ANSELMI, CAROLINE; THIM, GILMAR PATROCINIO; BOTTINO, MARCO C.; BORGES, ALEXANDRE LUIZ SOUTO; TRICHES, ELIANDRA DE SOUSA. Effect of Co, Cu, and Zn ions on the bioactivity and antibacterial properties of a borate bioactive glass. Journal of Non-Crystalline Solids, v. 622, p. 12-pg., 2023-09-25. (20/12874-9, 19/19594-4, 19/10877-3, 22/12217-3, 20/12507-6)

DE SOUZA, JOYCE R.; KUKULKA, ELISA C.; KITO, LETICIA T.; ALVES, MARIANA DE SA; DOS SANTOS, VERONICA R.; TRICHES, ELIANDRA S.; VASCONCELLOS, LUANA M. R.; THIM, GILMAR P.; CAMPOS, TIAGO M. B.; BORGES, ALEXANDRE L. S.. Electrospun Polylactic Acid/Polyethylene Glycol/ Silicate-Chlorinated Bioactive Glass Composite Scaffolds for Potential Bone Regeneration. POLYMERS FOR ADVANCED TECHNOLOGIES, v. 35, n. 11, p. 11-pg., 2024-11-01. (19/19594-4, 19/10877-3, 20/12507-6, 20/12874-9)

DOS SANTOS, VERONICA RIBEIRO; CAMPOS, TIAGO MOREIRA BASTOS; ANSELMI, CAROLINE; DE SOUZA, JOYCE RODRIGUES; LEMES, ANA PAULA; THIM, GILMAR PATROCINIO; BOTTINO, MARCO CICERO; BORGES, ALEXANDRE LUIZ SOUTO; TRICHES, ELIANDRA DE SOUSA. Development of PHBV electrospun fibers containing a borate bioactive glass doped with Co, Cu, and Zn for wound dressings. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, v. 112, n. 8, p. 13-pg., 2024-08-01. (20/12507-6, 20/12874-9, 19/19594-4, 19/10877-3, 22/12217-3)

MELO, MARCIA CRISTINA BEZERRA; SPIRANDELI, BRUNO ROBERTO; BARBOSAA, LUCAS; DOS SANTOS, VERONICA RIBEIRO; DE CAMPOS, TIAGO MOREIRA BASTOS; THIM, GILMAR PATROCINIO; TRICHES, ELIANDRA DE SOUSA. Enhanced mechanical strength and bioactivity of 3D-printed (3-TCP scaffolds coated with bioactive glasses. JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS, v. 163, p. 10-pg., 2024-12-06. (20/12874-9, 20/12507-6, 19/19594-4, 23/16554-7)

B. R. SPIRANDELI; E. F. MARTINS; L. R. M. DONA; R. G. RIBAS; T. M. B. CAMPOS; E. ESPOSITO; G. P. THIM; D. B. TADA; E. S. TRICHÊS. Synergistic Effect of Incorporation of BG 45S5 and Silver Nanoparticles on β-TCP Scaffolds: Structural Characterization and Evaluation of Antimicrobial Activity and Biocompatibility. MATERIALS RESEARCH-IBERO-AMERICAN JOURNAL OF MATERIALS, v. 26, 2023-06-19. (19/19594-4)

DE SIQUEIRA, LILIAN; DOS SANTOS, VERONICA RIBEIRO; DE ARAUJO, JULIANI CAROLINE RIBEIRO; DE OLIVEIRA FILHO, HUGO GUTEMBERG PATINO; DE VASCONCELLOS, LUANA MAROTTA REIS; TRICHES, ELIANDRA DE SOUSA; BORGES, ALEXANDRE LUIZ SOUTO. Ultrathin Polymer Fibers Coated with an Amorphous SiO₂-CaO-P₂O₅ Bioactive Powders for Biomedical Applications. FIBERS AND POLYMERS, v. N/A, p. 12-pg., 2023-07-31. (19/19594-4, 21/14277-0)

DOS SANTOS, VERONICA RIBEIRO; CAMPOS, TIAGO MOREIRA BASTOS; THIM, GILMAR PATROCINIO; BORGES, ALEXANDRE LUIZ SOUTO; TRICHES, ELIANDRA DE SOUSA. Glycol thermal synthesis of the 45B5 bioactive borate glass: Structural, physical, and apatite mineralization in vitro. CERAMICS INTERNATIONAL, v. 49, n. 7, p. 13-pg., 2023-02-21. (20/12507-6, 19/19594-4, 20/12874-9, 19/10877-3)

DE SOUZA, JOYCE RODRIGUES; KUKULKA, ELISA CAMARGO; DOS SANTOS, VERONICA RIBEIRO; KITO, LETICIA TERUMI; TRICHES, ELIANDRA DE SOUSA; THIM, GILMAR PATROCINIO; BORGES, ALEXANDRE LUIZ SOUTO; CAMPOS, TIAGO MOREIRA BASTOS. Enhancing bone regeneration: Exploring the potential of silicate chlorinated bioactive glasses and dehydration mechanisms. Journal of Non-Crystalline Solids, v. 631, p. 11-pg., 2024-03-10. (19/19594-4, 19/10877-3, 20/12507-6, 20/12874-9)

DOS REIS-PRADO, ALEXANDRE HENRIQUE; DE SOUZA, JOYCE RODRIGUES; TRICHES, ELIANDRA DE SOUSA; FURQUIM, ELISA MARA DE ABREU; DAL-FABBRO, RENAN; BENETTI, FRANCINE; BOTTINO, MARCO C.. Bioactive glasses for bone tissue engineering: a bibliometric study of the top 100-most cited papers. ODONTOLOGY, v. N/A, p. 43-pg., 2024-12-05. (19/19594-4)

AMARAL, SUELEN SIMOES; LIMA, BEATRIZ SAMARA DE SOUSA; AVELINO, SARAH OLIVEIRA MARCO; SPIRANDELI, BRUNO ROBERTO; CAMPOS, TIAGO MOREIRA BASTOS; THIM, GILMAR PATROCINIO; TRICHES, ELIANDRA DE SOUSA; DO PRADO, RENATA FALCHETE; DE VASCONCELLOS, LUANA MAROTTA REIS. beta-TCP/S53P4 Scaffolds Obtained by Gel Casting: Synthesis, Properties, and Biomedical Applications. BIOENGINEERING-BASEL, v. 10, n. 5, p. 19-pg., 2023-05-16. (20/07914-1, 19/19594-4)

OLIVEIRA, RODRIGO L. M. S.; FERRAZ, MARCOS C.; CARDOSO, LAIS MEDEIROS; LI, ZHONGRUI; ALBERS, ANA PAULA F.; BOTTINO, MARCO C.; TRICHES, ELIANDRA S.. 3D printing of strontium-enriched biphasic calcium phosphate scaffolds for bone regeneration. JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS, v. 160, p. 10-pg., 2024-09-06. (19/19594-4, 21/05259-9, 22/12217-3)

DOS SANTOS, VERONICA RIBEIRO; DE CAMPOS, TIAGO MOREIRA BASTOS; DE MACEDO, ERENILDA FERREIRA; DE CENA, GABRIELLE LUPETI; LEMES, ANA PAULA; THIM, GILMAR PATROCINIO; TADA, DAYANE BATISTA; CONCEICAO, KATIA; BORGES, ALEXANDRE LUIZ SOUTO; TRICHES, ELIANDRA DE SOUSA. PHBV Wound Dressing Containing 45B5 Borate Bioactive Glass: Effect of the Particle Incorporation Method on the Cytocompatibility and Antibacterial Activity. MATERIALS RESEARCH-IBERO-AMERICAN JOURNAL OF MATERIALS, v. 27, p. 13-pg., 2024-01-01. (20/12874-9, 20/12507-6, 19/10877-3, 19/19594-4)