Revealing transcriptome and proteome data for characterization of peptidomes and bioactive peptides in organisms from bycatch of shrimp trawling

• Grant number: 19/16023-6
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2020
• End date: September 30, 2022
• Field of knowledge: Biological Sciences - Pharmacology - Biochemical and Molecular Pharmacology
• Principal Investigator: Leandro Mantovani de Castro
• Grantee: Leandro Mantovani de Castro
• Host Institution: Instituto de Biociências (IB-CLP). Universidade Estadual Paulista (UNESP). Campus Experimental do Litoral Paulista. São Vicente , SP, Brazil
• Associated researchers: Emer Suavinho Ferro

Abstract

Marine organisms are considered an important source of bioactive compounds, among which we can highlight the class of peptides that play distinct biological functions in the prevention and treatment of various diseases, such as infectious, neurological, cardiovascular, metabolic and oncological. Even the face of the vast marine biodiversity, only a small part of organisms have been studied, and most remain with little molecular information necessary for the discovery of new molecules of therapeutic interest. In this project our objective is the bioprospecting of peptides through the generation of transcriptome and proteomic data of marine organisms of the Brazilian biodiversity, constituent of the bycatch of shrimp trawling fisheries. For this purpose, we will use New Generation Sequencing (NGS) and mass spectrometry for the characterization of transcriptomes, proteomes and peptidomes of these animals. Additionally, the peptides identified in fractions with bioactivity will be chemically synthesized and the cellular mechanisms involved and their therapeutic potential investigated. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FIAMETTI, LOUISE OLIVEIRA; CORREA, CLAUDIA NEVES; CASTRO, LEANDRO MANTOVANI DE. Peptide Profile of Zebrafish Brain in a 6-OHDA-Induced Parkinson Model. ZEBRAFISH, v. 18, n. 1, FEB 2021. (19/17433-3, 19/16023-6)

CORREA, CLAUDIA NEVES; FIAMETTI, LOUISE OLIVEIRA; DE BARROS, GABRIEL MARQUES; DE CASTRO, LEANDRO MANTOVANI. Revealing Natural Intracellular Peptides in Gills of Seahorse Hippocampus reidi. BIOMOLECULES, v. 13, n. 3, p. 15-pg., 2023-03-01. (19/16023-6)

MAZZI ESQUINCA, MARIA EDUARDA; CORREA, CLAUDIA NEVES; MARQUES DE BARROS, GABRIEL; MONTENEGRO, HORACIO; MANTOVANI DE CASTRO, LEANDRO. Multiomic Approach for Bioprospection: Investigation of Toxins and Peptides of Brazilian Sea Anemone Bunodosoma caissarum. MARINE DRUGS, v. 21, n. 3, p. 26-pg., 2023-03-01. (21/01286-1, 19/16023-6)

CORREA, CLAUDIA NEVES; FIAMETTI, LOUISE OLIVEIRA; MAZZI ESQUINCA, MARIA EDUARDA; DE CASTRO, LEANDRO MANTOVANI. Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, n. 177, NOV 2021. (21/01286-1, 19/17433-3, 19/16023-6)