Synthesis, studies and applications of fluorescent and circular dichroic probes for characterization of interactions with proteins, DNA and determination of enzymatic activity

• Grant number: 19/18445-5
• Support Opportunities: Regular Research Grants
• Duration: February 01, 2021 - January 31, 2023
• Field of knowledge: Biological Sciences - Biochemistry - Enzymology
• Principal Investigator: Valdecir Farias Ximenes
• Grantee: Valdecir Farias Ximenes
• Host Institution: Faculdade de Ciências (FC). Universidade Estadual Paulista (UNESP). Campus de Bauru. Bauru , SP, Brazil
• Associated researchers: Aguinaldo Robinson de Souza ; Nelson Henrique Morgon

Abstract

Detection, monitoring and quantification of molecular processes in simple systems, such as biochemical reactions in isolated systems, or complex ones, such as those occurring within cells or supramolecular systems, occurs by contrasting between what is wanted and what is not wanted to see. In other words, by increasing the signal to noise ratio. In this scenario, fluorescence-based techniques stand out because they are the most used methods in chemical and biomedical field. Indeed, it would not be possible to imagine the development in areas as biochemistry, immunology, microbiology, cell biology, molecular biology etc, without the use of fluorescent markers capable of elucidating the expression of proteins, cytokines, metabolites, reactive oxygen and nitrogen species, release or uptake. ions, changes in intracellular pH, membrane potential, signaling pathways, transcription factors etc? This project fits in this field, since part of it is focused on the development and application of fluorescent probes for the following purposes: synthesis and study of fluorescent aminoquinolines for selective determination of enzymatic activity of the enzymes myeloperoxidase and eosinophil peroxidase; synthesis and study of fluorescent probe for DNA study via exciplex formation; energy transfer study (FRET) for elucidating interaction of fluorescent probes with albumin; solvatochromic studies with the probes 4-(Dicyanomethylene)-2-methyl-6-(4-dimethylaminostyryl)-4H-pyran and 4-dimethylamino-²-nitrostyrene and application for determination of dielectric constant inside protein. The project also aims the synthesis and development of probes susceptible to induction of circular dichroism signal. In this case we aim to develop a method to monitor the displacement of the probes between two proteins as a function of time and to apply to the characterization study of amyloid aggregates. Also, in this line, the circular dichroism signal induced in rose Bengal dye will be used to study its movement in the protein. All the studies mentioned above will have their theoretical counterpart by ab initio methods which will seek to understand the phenomena at molecular level, simulations of absorption, emission and circular dichroism spectra of free and complexed probes to macromolecules. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ZAZERI, GABRIEL; RIBEIRO POVINELLI, ANA PAULA; PAVAN, NATHALIA M.; DE CARVALHO, DANIELLA ROMANO; CARDOSO, CARMEN LUCIA; XIMENES, VALDECIR FARIAS. Experimental studies and computational modeling on cytochrome c reduction by quercetin: The role of oxidability and binding affinity. Journal of Molecular Structure, v. 1244, NOV 15 2021. (14/50926-0, 19/18445-5)

ZERAIK, MARIA LUIZA; PAULI, IVANI; DUTRA, LUIZ A.; CRUZ, RAQUEL S.; VALLI, MARILIA; PARACATU, LUANA C.; DE FARIA, CAROLINA M. Q. G.; XIMENES, VALDECIR F.; REGASINI, LUIS O.; ANDRICOPULO, ADRIANO D.; et al. Identification of a Prenyl Chalcone as a Competitive Lipoxygenase Inhibitor: Screening, Biochemical Evaluation and Molecular Modeling Studies. Molecules, v. 26, n. 8, APR 2021. (10/01506-7, 13/07600-3, 11/03017-6, 19/05967-3, 19/18445-5, 09/15457-0, 14/50926-0)

XIMENES, VALDECIR F.; XIMENES, THOMAZ P.; MORGON, NELSON H.; DE SOUZA, AGUINALDO R.. Taurine Chloramine and Hydrogen Peroxide as a Potential Source of Singlet Oxygen for Topical Application. Photochemistry and Photobiology, MAR 2021. (16/20549-5, 19/18445-5)

ALVES DA SILVA, BRUNA TERRA; PELOI, KAREN ELAINE; XIMENES, VALDECIR FARIAS; NAKAMURA, CELSO VATARU; SILVA LAUTENSCHLAGER, SUELI DE OLIVEIRA. 2-acetylphenothiazine protects L929 fibroblasts against UVB-induced oxidative damage. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 216, MAR 2021. (14/50926-0, 19/18445-5)

BERTOZO, LUIZA DE CARVALHO; MASZOTA-ZIELENIAK, MARTYNA; BOLEAN, MAYTE; CIANCAGLINI, PIETRO; SAMSONOV, SERGEY A.; XIMENES, VALDECIR F.. inding of fluorescent dansyl amino acids in albumin: When access to the protein cavity is more important than the strength of bindin. DYES AND PIGMENTS, v. 188, APR 2021. (19/18445-5, 16/22014-1)

XIMENES, VALDECIR F.; XIMENES, THOMAZ P.; MORGON, NELSON H.; DE SOUZA, AGUINALDO R.. Taurine Chloramine and Hydrogen Peroxide as a Potential Source of Singlet Oxygen for Topical Application. Photochemistry and Photobiology, v. 97, n. 5, p. 963-970, SEP 2021. (19/18445-5, 16/20549-5)

DOS SANTOS, WILLIAN HENRIQUE; YOGUIM, MAURICIO IKEDA; DARE, REGINA GOMES; DA SILVA-FILHO, LUIZ CARLOS; LAUTENSCHLAGER, SUELI OLIVEIRA SILVA; XIMENES, VALDECIR FARIAS. Development of a caffeic acid-phthalimide hybrid compound for NADPH oxidase inhibition. RSC ADVANCES, v. 11, n. 29, p. 17880-17890, MAY 27 2021. (19/18445-5, 16/20549-5, 14/50926-0)

VALDECIR F. XIMENES; NATHALIA M. PAVAN; AGUINALDO R. DE SOUZA; GABRIEL A. BARROS; NELSON H. MORGON. Nicotine as a Catalyst for Chlorination Promoted by Hypochlorous Acid: Experimental and Theoretical Studies. Journal of the Brazilian Chemical Society, v. 34, n. 1, p. 63-68, 2023-01-06. (19/18445-5, 19/12294-5)