Multiple cellular and tissue biomarkers in guppy (Poecilia reticulata) after long-term exposure to glyphosate, LR microscystin and cylinderspermopsin associated to metal nanoparticles (NANOMBI)

• Grant number: 19/24180-4
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2020
• End date: August 31, 2023
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Inland Water Fishery Resources
• Agreement: CONFAP - National Council of State Research Support Foundations
• Principal Investigator: Marisa Narciso Fernandes
• Grantee: Marisa Narciso Fernandes
• Host Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
• Associated researchers: Daniela de Melo e Silva ; Emilia Celma de Oliveira Lima ; Wanessa Fernandes Carvalho

Abstract

Conservation of aquatic environments is essential for the survival of all species, including humans. Therefore, it is necessary to monitor, maintain and preserve the environmental health conditions. Anthropic actions are already present in all environments and are often associated with economic, productive and the subsistence needs with which we need to live and there is a need to act through mitigating measures that repair or even minimize environmental impacts. Among these impacts the high nitrogen and phosphorus levels resulted from the excessive use of agrochemicals, promote of exacerbated microalgae proliferation which, in turn, synthesize and secrete natural substances considered toxins. All these substances make the waters unfit for the consumption and survival of aquatic fauna. Therefore, researchers are looking for ways to effectively maintain aquatic health and consider numerous possibilities of nanotechnology application, by using metallic nanoparticles (NPMs), especially the ferric ones, as workable alternative to carry out the environmental remediation processes. The present proposal will perform the synthesis, characterization and functionalization of ferric NPMs with the objective to use them as pickup agents of glyphosate molecules and aquatic toxins as the microcystin-LR (Mic-LR) and the cylrospermopsin (CYN) and verify the effectiveness of functionalized NPMs to associate with the mentioned molecules. To investigate the action of these nanoparticles associated to toxic substances, fish considered models for toxicological analysis, the guppies (Poecilia reticulata) will be exposed for a long time (21 days) to NPMs associated with GLY, Mic-LR and CYN and, thereafter, multiples biomarkers (genotoxic, mutagenic, biochemical/fisiological and morphological) will be analyzed in the gills and liver. Groups of fish after exposure to such conditions will be left for a recovere for a period of 21 days and then, multiples biomarkers will be analyzed in the gills and liver to assess if in the complete absence of the tested agents the cellular and tissue behavior of these animals returned to the control levels. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA SILVA, VICTORIA COSTA; DE LIMA-FARIA, JOAO MARCOS; GUIMARAES, LUCAS NUNES; COSTA, MATHEUS SANTOS; DE LIMA, PHAMELLA NERES; SIMOES, KARINA; OLIVEIRA DE JESUS, LAZARO WENDER; TEIXEIRA DE SABOIA-MORAIS, SIMONE MARIA. Ovaries of guppies (Poecilia reticulata) investigated in pre-embryonic, embryonic and post-embryonic stages after exposure to maghemite nanoparticles (y-Fe2O3) associated with Roundup (R) and glyphosate, followed by recovery period evaluation. ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, v. 100, p. 12-pg., 2023-05-17. (19/24180-4)

DE LIMA-FARIA, JOAO MARCOS; GUIMARAES, LUCAS NUNES; DA SILVA, VICTORIA COSTA; SOUZA, IARA DA COSTA; FERNANDES, MARISA NARCISO; TEODORO MARTINEZ, DIEGO STEFANI; TEIXEIRA DE SABOIA-MORAIS, SIMONE MARIA. Distribution and behavior of lipid droplets in hepatic cells analyzed by variations of citochemical technique and scanning electron microscopy. METHODSX, v. 9, p. 9-pg., 2022-07-04. (19/24180-4)

DE LIMA-FARIA, JOAO MARCOS; DA SILVA, VICTORIA COSTA; CHEN, LEE CHEN; MARTINEZ, DIEGO SEFANI TEODORO; DE SABOIA-MORAIS, SIMONE MARIA TEIXEIRA. Co-exposure of iron oxide nanoparticles with glyphosate herbicides in Poecilia reticulata: Fish liver damages is reversible during iron accumulation and elimination period. Chemosphere, v. 328, p. 9-pg., 2023-04-07. (19/24180-4)