Multi-user equipment approved in grant 2019/26575-6: respirometry system with flow kit pump

• Grant number: 20/14441-2
• Support Opportunities: Multi-user Equipment Program
• Start date: February 01, 2021
• End date: January 31, 2028
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Animal Nutrition and Feeding
• Principal Investigator: Nilva Kazue Sakomura
• Grantee: Nilva Kazue Sakomura
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Pesquisadores principais: Marcos Macari
• Associated researchers: Alessandro de Mello Varani ; Alex Sandro Campos Maia ; Etienne Labussière ; Jacob van Milgen ; Jean Noblet ; Kênia Cardoso Bícego ; Mingan Choct ; Robert Mervyn Gous ; Robert Swick ; Sandro Cerrate ; Shu-Biao Wu
• Associated research grant: 19/26575-6 - Net energy system for poultry, AP.TEM
• EMU web page: Página do Equipamento Multiusuário não informada
• Type of equipment: Processos Biológicos - Crescimento e manipulação - Fermentação; Caracterização de Materiais - Análises Químicas - Monitoramento de gases; Caracterização e Análises de Amostras - Biomédica - Celular
• Manufacturer: Fabricante não informado
• Model: Modelo não informado

Abstract

The indirect calorimetry method, using the respirometry system, is employed for metabolic measurement in poultry birds, with the aim to evaluate the energy metabolism of poultry categories (broiler chicken. Laying hens, and broiler breeders) and the feed energy utilization of different feedstuff used un poultry production. The respirometry system proposed on the thematic project n. 2019/26575-6 consists of an open-circuit of respirometry chambers (internal dimension: 90×85×95 cm), equipped with a digital environmental temperature controller. The system of respirometry consists of a mass flow kit pump (FK-100, Sable System, Las Vegas) that pulls the air by negative pressure at a ventilation flow set depending on the animal's mass allocated in the chamber from the external environment (atmospheric air) to inside of each. A sub-sampler (SS4, Sable System, Las Vegas) conduces the air sample of 160 ml/min to the RH-100 analyzer (Sable System, Las Vegas) to register the water vapor pressure. Sequentially, the sample is drier on a Drierite (CaSO4) lineal-tube and conducted to paramagnetic oxygen analyzer (PA-10, Sable System, Las Vegas) and carbon dioxide analyzer based on the infrared principle (CA-10, Sable System, Las Vegas). The respirometry system consists of a group of chambers, programmed to the intermittent sequence of data collection, where the measurement can be done on the long time period of reading (approximately by 24 hours), recording the gas exchange (O2 consumption and CO2 production) data each second and averaging by minute to heat production (HP) calculations. This system is adapted to biological trials in poultry (in groups and individually). (AU)